Process for preparing acrylamide aqueous solution through direct method cell enzyme reaction
A technology for acrylamide and cellular enzymes, which is applied in the field of direct-method cellular enzyme reaction to produce acrylamide aqueous solution, can solve the problems of long production cycle, complicated process, environmental pollution and the like, and achieves the effects of low cost, simple process and high product purity
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Embodiment 1
[0032] Take 75ml of bio-fermentation liquid, separate it by centrifuge, the parameter is 7000rpm, control the chroma below 25, remove the supernatant, put the sediment into the reactor, then add deionized water to 725ml, adjust the pH value of the reaction system to 6 -8, the temperature was controlled at 15°C, and 300ml of chemically pure acrylonitrile was added to react for 240 minutes, and the reaction solution was quickly membrane-filtered, and the filtrate was taken to measure: AM% was 32.1% (mass ratio), AN% was 1.1 % (mass ratio), the conductivity is 1220 μs / cm, the chromaticity is 15, and the pH value is 6.8. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.
Embodiment 2
[0034] Take 75ml of bio-fermentation liquid and separate it by membrane. The cut-off pore size parameter of membrane separation is 60,000 molecular weight, and the chromaticity is controlled to be below 15. Put the heavy phase into the reactor, add deionized water to 725ml, adjust the pH value to 6-8, and react The temperature of the system was controlled at 25°C, and 300ml of chemically pure acrylonitrile was added for reaction. After 240 minutes of reaction, the reaction solution was rapidly passed through membrane filtration, and the filtrate was taken to measure: AM% was 33% (mass ratio), AN% was 0.12 % (mass ratio); the conductivity is 500 μs / cm, the chromaticity is 10, and the pH value is 7.1. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.
Embodiment 3
[0036] Take 75ml of bio-fermentation liquid and centrifuge in sequence at 8000rpm. The sediment is filtered through a membrane. The cut-off aperture parameter of the membrane separation is 50,000 molecular weight, and the chromaticity is controlled to be 5. The heavy phase is put into the reactor, and deionized water is added. to 725ml, adjust the pH value to 6-8, and control the ambient temperature at 18°C. Add 300ml of chemically pure acrylonitrile for hydration reaction. After 240 minutes of reaction, let the reaction solution pass through membrane filtration quickly, and take the filtrate to measure: AM % is 33% (mass ratio), AN% is not detected, the conductivity is 283 μs / cm, the chromaticity is 5, and the pH value is 7.5. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.
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