A kind of soluble-insoluble ucst type pmaac carrier, its immobilized enzyme and application

A carrier and solubility technology, applied in the direction of immobilized enzymes, immobilized on/in organic carriers, glycosylases, etc., can solve the problems of difficult recovery and low efficiency of cellulose hydrolysis, and achieve improved stability and effective It is beneficial to full contact and solves the effect of LCST type low-efficiency hydrolysis problem
CN108642040BActive Publication Date: 2020-09-25JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2020-09-25

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Abstract

The invention belongs to the technical field of immobilized enzymes, relates to a soluble-insoluble UCST type PMAAc vector as well as an immobilized enzyme and an application thereof, and in particular to a preparation method of the soluble-insoluble high critical solution temperature (UCST) type sodium polymethylacrylate (PMAAc) vector, an application of the immobilized enzyme and an applicationof an immobilized enzyme degraded cellulose material thereof. The preparation method comprises the following steps: dissolving acrylic acid and ammonium persulfate through de-ionized water; slowly dropping methacrylamide which is dissolved by de-ionized water; uniformly shaking an obtained solution, filling nitrogen until bubbling and transferring an obtained solution into a single-mouth flask; implementing vacuumizing in nitrogen protection; conducting a reaction under magnetic stirring; implementing centrifuging after conducting cooling; discarding supernatant liquid; and heating up a resuspended material via de-ionized water, so that the finished product (the PMAAc vector) is obtained. The vector provided by the invention is applicable to immobilized cellulase, and the vector is used for degrading cellulose materials; the immobilized enzyme prepared by the invention is excellent in storage stability and reuse effect; and efficiency of hydrolyzing and catalyzing the cellulose materials is also obviously improved.
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Description

technical field

[0001] The invention belongs to the technical field of immobilized enzymes, and relates to a UCST type PMAAc carrier, its immobilized enzyme and its application, in particular to a soluble-insoluble high critical solution temperature (UCST) type methacrylamide-acrylic acid copolymer (PMAAc ) carrier preparation method and application of immobilized cellulase and co-immobilized cellulase and β-glucosidase. Background technique

[0002] Cellulose, one of the most abundant resources on earth, can be converted into functionally diverse glucose through chemical and biological means. At present, the fossil energy crisis is becoming more and more serious, and the green way of converting biomass glucose into fuel ethanol is becoming a major research hotspot in the energy industry. Because the free enzyme is difficult to recycle, and after a long time of enzymatic hydrolysis, the enzyme activity is easy to lose, these are the main obstacles inhibiting its industrial ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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