17 results about "Immobilized cellulase" patented technology

The invention belongs to the technical field of lignocellulose biomass enzymolysis saccharification, and particularly relates to a method and device for lignocellulose biomass enzymolysis through immobilized cellulase, and the method for lignocellulose biomass enzymolysis through immobilized cellulase comprises the following steps that lignocellulose biomass is pretreated; the method comprises the following steps: immobilizing cellulase through polydopamine modified foamed nickel to obtain polydopamine modified foamed nickel immobilized cellulase, and mixing the polydopamine modified foamed nickel immobilized cellulase with pretreated lignocellulose biomass to form an enzymolysis reaction system; adjusting the reaction temperature of the enzymolysis reaction system, carrying out enzymolysis saccharification reaction, separating saccharification liquid from enzymolysis residues, carrying out magnetic adsorption treatment on the enzymolysis residues, and recycling the polydopamine modified foam nickel immobilized cellulase. According to the method, the economic feasibility of lignocellulose biomass enzymolysis is improved, and efficient enzymolysis of lignocellulose biomass is realized.

The invention discloses a method for immobilizing cellulase by using a Ti3O5-based composite photothermal conversion material and application. The material is composed of a Ti3O5 core coated with polydopamine and a glycidyl methacrylate modified azobenzene polymer brush grafted on the surface of the Ti3O5 core. The preparation method comprises the following steps: forming a PDA layer on the surface of Ti3O5 through dopamine auto-polymerization, synthesizing a gAZO polymer brush, and finally modifying the gAZO onto the PDA-coated Ti3O5 through photo-initiation grafting reaction. The material disclosed by the invention can realize local temperature regulation and control at 35-50 DEG C under the irradiation of visible light of 100-150 mW / cm; when being used for immobilizing cellulase, the immobilized cellulase can be locally heated through illumination in the synchronous saccharification and fermentation process, so that the problem that the optimal saccharification and fermentation temperatures are not matched is solved, and the SSF efficiency is improved; the process is simple, and a novel controllable temperature control strategy is provided for the bio-refinery process.

The invention provides an enzyme catalytic synthesis process of bio-based water-based resin for textile slurry, and relates to the technical field of water treatment. The enzyme-catalyzed synthesis process of the bio-based water-based resin for the textile size comprises the following steps: S1, based on a plant cellulose raw material, adopting a microwave-assisted pretreatment process, destroying a cellulose crystal structure through 2.45 GHz microwave radiation, taking mesoporous silica as a carrier, and adding a 1% dilute sulfuric acid solution to promote hemicellulose hydrolysis, the microwave activated cellulose slurry is generated. By combining microwave-assisted pretreatment with dilute sulphuric acid hydrolysis, the activation efficiency of cellulose is remarkably improved, by destroying a crystal structure and selectively hydrolyzing hemicellulose, active sites of a subsequent enzyme catalytic reaction are fully exposed, the reaction energy consumption and time are reduced, an immobilized cellulase and laccase concerted catalysis system realizes directional grafting of acrylate monomers, and the specific surface area is increased. The mesoporous silica of the enzyme carrier provides a stable microenvironment, and the catalytic efficiency and the reuse rate are improved.

The invention discloses a rhizoma polygonati polysaccharide extraction method, and belongs to the technical field of rhizoma polygonati polysaccharide extraction, the method comprises the following steps: S1, preparing Fe3O4-chitosan magnetic nanoparticles, and jointly immobilizing cellulase and pectinase on the Fe3O4-chitosan magnetic nanoparticles to obtain Fe3O4-chitosan magnetic nanoparticles co-immobilized by the cellulase and the pectinase; the preparation method comprises the following steps: dispersing Fe3O4-chitosan magnetic nanoparticles co-immobilized by cellulase and pectinase in a protective agent solution, uniformly stirring, adding a proper amount of cross-linking agent glutaraldehyde, reacting at room temperature for 2-4 hours to form a first layer of protective film, and repeating the steps to form a plurality of layers of protective films (generally 2-3 layers); and the Fe3O4-chitosan magnetic nanoparticles which are co-immobilized by the cellulase and the pectinase and coated by the protective agent are obtained. The extraction rate and the purity of the polygonatum polysaccharide are improved, and meanwhile the content of impurities is reduced.

The application belongs to the technical field of enzyme engineering and new material cross, and particularly relates to a kind of immobilized cellulase of magnetic mesoporous core-shell material and preparation method and application. The immobilized cellulase is obtained by physical adsorption of cellulase with magnetic mesoporous core-shell material F5HPU-46 as carrier. F5HPU-46 is a unique core-shell structure with paramagnetic Fe3O4 nanoparticles as core layer and UIO-66 mesoporous shell layer with stable structure, which has the dual advantages of magnetic response and high specific surface area mesoporous structure. The relative enzyme activity of the immobilized cellulase in the application is increased to 119% under acidic conditions at pH 4, while the relative enzyme activity of free enzyme is decreased to 75%.

The present invention refers to an ultrasonic baffled hydrothermal (UBH) reactor for the pretreatment of lignocellulosic biomass, using rotating baffles (180) and blades (182) driven by a motor (73), surrounding heating coils (181) and ultrasonic probes (177, 123). The invention also refers to immobilized cellulase using metal-organic frameworks (MOF). This invention also refers to the counter-current saccharification process. The invention further refers to a thermosiphon which can be used couples with a falling film in a distillation. Additionally, it also refers to a process for producing bioethanol using said UBH reactor, the immobilized cellulase in a counter-current process and a falling film e coupled with the thermosiphon. A system for producing bioethanol comprising the UBH reactor, a saccharification counter-current process, falling films and a thermosiphon is also presented. The uses of the UBH reactor, the enzyme and the thermosiphon are also proposed.

The invention relates to the technical field of biological enzymes, and discloses a preparation process of immobilized cellulase for degrading petroleum drilling fluid, which comprises the following steps: step 1, preparing a cellulase component; step 2, preparing a molecular chaperone component; step 3, preparing protein organic components; and step 4, preparing an immobilized cellulase finished product. The protein organic component is prepared by the following steps: uniformly mixing a cellulase component and a molecular chaperone component according to a mass ratio of (1.5: 1)-(3: 1), and dissolving the mixture in a phosphate buffer solution (pH 7.4) to obtain the protein organic component; the immobilized cellulase finished product is prepared by the following steps: uniformly mixing protein organic components with a manganese sulfate solution (0.1 M) according to a volume ratio of 2: 1-4: 1, standing at 4 DEG C for 2-3 days, centrifuging, collecting precipitates, and performing freeze-drying treatment to obtain the immobilized cellulase finished product. The invention provides an efficient and environment-friendly biodegradation strategy capable of being used for degrading the petroleum drilling fluid, and the improvement of the oil and gas exploitation efficiency is facilitated.

The present application relates to papermaking auxiliary technical field, specifically to a kind of papermaking with fibrous property improved biological enzyme composition and preparation method thereof, comprising: immobilized xylanase 10 parts by weight-40 parts by weight, enzyme activity 1000U / g-1500U / g;Immobilized cellulase 5 parts by weight-25 parts by weight, enzyme activity 700U / g-1000U / g;Immobilized pectinase 6 parts by weight-35 parts by weight, enzyme activity 500U / g-800U / g;Cassava starch and diatomite compound carrier 5 parts by weight-20 parts by weight, wherein the mass ratio of cassava starch and diatomite is (2-5):1;Composite stabilizer 0.5 parts by weight-8 parts by weight, which is composed of trehalose and propylene glycol according to mass ratio (0.5-2):1;Chitosan auxiliary enzyme agent 1 parts by weight-5 parts by weight;And deionized water.The present application solves the problems of insufficient stability, single modification effect and limited application range of existing papermaking biological enzyme preparation by multi-enzyme synergistic compounding, differential immobilization, optimized combination of compound carrier and synergistic adjuvant, and has outstanding application advantages.

The present invention discloses a preparation method of immobilized cellulase magnetic biochar and its application in tobacco stem composting fermentation, belonging to the field of fertilizer fermentation technology, and the steps are: carboxylation of biochar; photocatalytic activation of biochar; preparation of modified Fe3O4@SiO2‑NC nanoparticles to prepare new magnetic biochar; immobilization of cellulase. The composting fermentation method is to mix tobacco stems, cow dung, urea, corn flour, and fermentation aids as a tobacco stem compost matrix, add immobilized cellulase and mix evenly, add water, and ferment for 30 days. The thermal stability of the cellulase prepared by the present invention after being immobilized on the magnetic biochar material is improved. At 50°C, the cellulase activity is increased by 12%, and after 60°C, the cellulase activity is increased by 50%; the application of the cellulase immobilized magnetic biochar material in the tobacco stem and cow dung composting fermentation process can shorten the cycle and significantly improve the cellulose degradation rate, organic matter content, humic acid content, and tobacco seedling germination rate.

The present invention relates to the technical field of essential oil extraction, and in particular to an enzyme-assisted rose essential oil preparation method and rose essential oil. The rose essential oil preparation method comprises the following steps: mixing rose petals with water, homogenizing to prepare rose homogenate; adding immobilized cellulase to the rose homogenate, adjusting the pH to 4.0-5.0, performing enzymolysis treatment, adjusting the pH to 7.4-8.0, taking out the immobilized cellulase, and obtaining an enzymolysis homogenate; the immobilized cellulase is a super-hydrophilic membrane with cellulase connected to the surface through polyhistidine; steam distilling the enzymolysis homogenate, collecting the distillate, performing oil-water separation, and obtaining rose essential oil. In the preparation method of the present invention, a super-hydrophilic membrane with cellulase connected to the surface through polyhistidine is used as an immobilized cellulase, which can improve the enzymolysis efficiency and reduce the loss of enzymolysis homogenate components when the immobilized cellulase is separated, thereby achieving a higher yield of rose essential oil.

The present invention refers to an ultrasonic baffled hydrothermal (UBH) reactor for the pretreatment of lignocellulosic biomass, using rotating baffles (180) and blades (182) driven by a motor (73), surrounding heating coils (181) and ultrasonic probes (177, 123). The invention also refers to immobilized cellulase using metal-organic frameworks (MOF). This invention also refers to the counter-current saccharification process. The invention further refers to a thermosiphon which can be used couples with a falling film in a distillation. Additionally, it also refers to a process for producing bioethanol using said UBH reactor, the immobilized cellulase in a counter-current process and a falling film e coupled with the thermosiphon. A system for producing bioethanol comprising the UBH reactor, a saccharification counter-current process, falling films and a thermosiphon is also presented. The uses of the UBH reactor, the enzyme and the thermosiphon are also proposed.

The invention discloses a preparation method for synthesizing gastrodin and gastrodin through double-enzyme catalysis and application of the gastrodin and the gastrodin, and belongs to the technical field of compound extraction. The technical problems to be solved are that the chemical synthesis process of gastrodin and gastrodigenin is relatively high in safety risk, more in toxic and harmful reagent solvent residue, easy to generate by-products, limited in yield and low in purity, time-consuming and labor-consuming in extraction and separation from traditional Chinese medicinal materials, long in extraction time, low in extraction yield and purity, and low in effective content in medicines. According to the technical scheme, the method is characterized in that immobilized cellulase and lipase are adopted as biocatalysts to catalyze parishin compounds in the gastrodia elata extract to be converted into gastrodin and gastrodin, and the content of gastrodin and gastrodin in the gastrodia elata extract is increased, so that the drug effect is improved. The preparation process is green and environment-friendly, high selectivity is achieved, large-scale production can be achieved, the extraction efficiency and purity are improved, and the cost is reduced.

The invention discloses a preparation method and application of a bletilla striata component extract for improving diabetic foot ulcer pain, and the method comprises the following steps: S1, washing bletilla striata tubers with deionized water, air-drying, and crushing to obtain bletilla striata powder; s2, adding the porous magnetic microsphere immobilized cellulase into deionized water, performing ultrasonic dispersion, adding bletilla striata powder, and performing stirring reaction under ultrasonic assistance and heating; s3, filtering and collecting filtrate 1, adding filter residues into deionized water, stirring and reacting under ultrasonic assistance and heating, filtering and collecting filtrate 2, combining and collecting the filtrate 1 and the filtrate 2, and concentrating to obtain a crude bletilla striata extracting solution; and S4, adding ethanol into the crude bletilla striata extracting solution, stirring, standing overnight, centrifuging, washing precipitate with ethanol, re-dissolving in deionized water, stirring, filtering, ultra-filtering and concentrating the obtained solution, then adding ethanol, stirring, standing overnight, centrifuging, washing precipitate with ethanol, and drying to obtain the bletilla striata component extract.

The invention belongs to the technical field of biomass resource utilization, and discloses cardamine violifolia processing by-product-based charcoal immobilized cellulase as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing ferric chloride with the cardamine violifolia processing byproduct, and performing pyrolysis carbonization treatment to obtain a pyrolysis product; the pyrolysis product is subjected to acid pickling, water washing and drying treatment in sequence, and charcoal is obtained; and carrying out immobilization treatment on cellulase by taking the biochar as a carrier to obtain the cardamine violifolia processing byproduct-based biochar immobilized cellulase. The method provided by the invention not only realizes resource utilization of cardamine violifolia processing byproducts, but also provides a new path for preparation of immobilized cellulase with high loading capacity and high stability in the field of enzymatic preparation of glucose.

The utility model relates to the technical field of bioengineering, in particular to an efficient cellulase immobilization device which comprises a reaction tank body, a seal head arranged at the top of the reaction tank body and a seal bottom arranged at the bottom of the reaction tank body, a drip tube, a feed port and an exhaust port are arranged on the seal head, a discharge port is arranged on the seal bottom, and a jacket is arranged on the outer wall of the reaction tank body. A heat preservation layer is arranged on the outer wall of the jacket, a guide rail is arranged in the reaction tank body, a pulley is slidably mounted on the guide rail, a driving motor is mounted on the pulley, the instillation pipe penetrates through the end socket and extends downwards, the liquid outlet end of the instillation pipe is connected to the pulley, and the pulley is used for driving the liquid outlet end of the instillation pipe to reciprocate along the guide rail. According to the utility model, efficient immobilization treatment can be carried out on free cellulase, the liquid outlet end of the instillation tube can reciprocate on the guide rail at a constant speed along with the pulley, and the instillation speed of the instillation tube is uniform and stable, so that the prepared gel beads for immobilizing cellulase are uniform in texture, size and shape.

The invention provides a cervus elaphus linnaeus health-care wine for dispelling wind and clearing damp and a preparation method thereof, and belongs to the technical field of health-care products. Sequentially cleaning, airing, mixing, crushing and sieving hairy antler, radix angelicae pubescentis, radix saposhnikoviae, notopterygium root, herba taxilli, eucommia ulmoides, radix achyranthis bidentatae, astragalus membranaceus, mulberry, cassia twig and liquorice to obtain mixed powder, dispersing the mixed powder and immobilized cellulase into white spirit with the alcoholic strength of 5-10% vol, and soaking for 4-6 hours to obtain an enzymolysis system; transferring the enzymolysis system into Baijiu with the alcoholic strength of 50% vol, soaking for 15-20 days, and sequentially filtering, centrifuging and concentrating to obtain a concentrated solution; mixing rock sugar, honey and purified water to obtain a sugar solution, mixing the concentrated solution, the sugar solution, 60% vol fen-flavor base wine and purified water, and blending the alcoholic strength to 35% vol to obtain a mixed solution; and sequentially aging, filtering and sterilizing the mixed solution to obtain the wind-dispelling and dampness-eliminating cervus elaphus linnaeus health-care wine. And the immobilized cellulase is adopted to carry out enzymolysis treatment on the mixed powder, so that the preparation time is shortened, and the preparation efficiency is improved.