10 results about "Immobilized cellulase" patented technology

The invention discloses a method for immobilizing cellulase by using a Ti3O5-based composite photothermal conversion material and application. The material is composed of a Ti3O5 core coated with polydopamine and a glycidyl methacrylate modified azobenzene polymer brush grafted on the surface of the Ti3O5 core. The preparation method comprises the following steps: forming a PDA layer on the surface of Ti3O5 through dopamine auto-polymerization, synthesizing a gAZO polymer brush, and finally modifying the gAZO onto the PDA-coated Ti3O5 through photo-initiation grafting reaction. The material disclosed by the invention can realize local temperature regulation and control at 35-50 DEG C under the irradiation of visible light of 100-150 mW / cm; when being used for immobilizing cellulase, the immobilized cellulase can be locally heated through illumination in the synchronous saccharification and fermentation process, so that the problem that the optimal saccharification and fermentation temperatures are not matched is solved, and the SSF efficiency is improved; the process is simple, and a novel controllable temperature control strategy is provided for the bio-refinery process.

The invention provides an enzyme catalytic synthesis process of bio-based water-based resin for textile slurry, and relates to the technical field of water treatment. The enzyme-catalyzed synthesis process of the bio-based water-based resin for the textile size comprises the following steps: S1, based on a plant cellulose raw material, adopting a microwave-assisted pretreatment process, destroying a cellulose crystal structure through 2.45 GHz microwave radiation, taking mesoporous silica as a carrier, and adding a 1% dilute sulfuric acid solution to promote hemicellulose hydrolysis, the microwave activated cellulose slurry is generated. By combining microwave-assisted pretreatment with dilute sulphuric acid hydrolysis, the activation efficiency of cellulose is remarkably improved, by destroying a crystal structure and selectively hydrolyzing hemicellulose, active sites of a subsequent enzyme catalytic reaction are fully exposed, the reaction energy consumption and time are reduced, an immobilized cellulase and laccase concerted catalysis system realizes directional grafting of acrylate monomers, and the specific surface area is increased. The mesoporous silica of the enzyme carrier provides a stable microenvironment, and the catalytic efficiency and the reuse rate are improved.

The invention discloses a rhizoma polygonati polysaccharide extraction method, and belongs to the technical field of rhizoma polygonati polysaccharide extraction, the method comprises the following steps: S1, preparing Fe3O4-chitosan magnetic nanoparticles, and jointly immobilizing cellulase and pectinase on the Fe3O4-chitosan magnetic nanoparticles to obtain Fe3O4-chitosan magnetic nanoparticles co-immobilized by the cellulase and the pectinase; the preparation method comprises the following steps: dispersing Fe3O4-chitosan magnetic nanoparticles co-immobilized by cellulase and pectinase in a protective agent solution, uniformly stirring, adding a proper amount of cross-linking agent glutaraldehyde, reacting at room temperature for 2-4 hours to form a first layer of protective film, and repeating the steps to form a plurality of layers of protective films (generally 2-3 layers); and the Fe3O4-chitosan magnetic nanoparticles which are co-immobilized by the cellulase and the pectinase and coated by the protective agent are obtained. The extraction rate and the purity of the polygonatum polysaccharide are improved, and meanwhile the content of impurities is reduced.

The application belongs to the technical field of enzyme engineering and new material cross, and particularly relates to a kind of immobilized cellulase of magnetic mesoporous core-shell material and preparation method and application. The immobilized cellulase is obtained by physical adsorption of cellulase with magnetic mesoporous core-shell material F5HPU-46 as carrier. F5HPU-46 is a unique core-shell structure with paramagnetic Fe3O4 nanoparticles as core layer and UIO-66 mesoporous shell layer with stable structure, which has the dual advantages of magnetic response and high specific surface area mesoporous structure. The relative enzyme activity of the immobilized cellulase in the application is increased to 119% under acidic conditions at pH 4, while the relative enzyme activity of free enzyme is decreased to 75%.

The present application relates to papermaking auxiliary technical field, specifically to a kind of papermaking with fibrous property improved biological enzyme composition and preparation method thereof, comprising: immobilized xylanase 10 parts by weight-40 parts by weight, enzyme activity 1000U / g-1500U / g;Immobilized cellulase 5 parts by weight-25 parts by weight, enzyme activity 700U / g-1000U / g;Immobilized pectinase 6 parts by weight-35 parts by weight, enzyme activity 500U / g-800U / g;Cassava starch and diatomite compound carrier 5 parts by weight-20 parts by weight, wherein the mass ratio of cassava starch and diatomite is (2-5):1;Composite stabilizer 0.5 parts by weight-8 parts by weight, which is composed of trehalose and propylene glycol according to mass ratio (0.5-2):1;Chitosan auxiliary enzyme agent 1 parts by weight-5 parts by weight;And deionized water.The present application solves the problems of insufficient stability, single modification effect and limited application range of existing papermaking biological enzyme preparation by multi-enzyme synergistic compounding, differential immobilization, optimized combination of compound carrier and synergistic adjuvant, and has outstanding application advantages.

The present invention discloses a preparation method of immobilized cellulase magnetic biochar and its application in tobacco stem composting fermentation, belonging to the field of fertilizer fermentation technology, and the steps are: carboxylation of biochar; photocatalytic activation of biochar; preparation of modified Fe3O4@SiO2‑NC nanoparticles to prepare new magnetic biochar; immobilization of cellulase. The composting fermentation method is to mix tobacco stems, cow dung, urea, corn flour, and fermentation aids as a tobacco stem compost matrix, add immobilized cellulase and mix evenly, add water, and ferment for 30 days. The thermal stability of the cellulase prepared by the present invention after being immobilized on the magnetic biochar material is improved. At 50°C, the cellulase activity is increased by 12%, and after 60°C, the cellulase activity is increased by 50%; the application of the cellulase immobilized magnetic biochar material in the tobacco stem and cow dung composting fermentation process can shorten the cycle and significantly improve the cellulose degradation rate, organic matter content, humic acid content, and tobacco seedling germination rate.

The invention discloses a preparation method for synthesizing gastrodin and gastrodin through double-enzyme catalysis and application of the gastrodin and the gastrodin, and belongs to the technical field of compound extraction. The technical problems to be solved are that the chemical synthesis process of gastrodin and gastrodigenin is relatively high in safety risk, more in toxic and harmful reagent solvent residue, easy to generate by-products, limited in yield and low in purity, time-consuming and labor-consuming in extraction and separation from traditional Chinese medicinal materials, long in extraction time, low in extraction yield and purity, and low in effective content in medicines. According to the technical scheme, the method is characterized in that immobilized cellulase and lipase are adopted as biocatalysts to catalyze parishin compounds in the gastrodia elata extract to be converted into gastrodin and gastrodin, and the content of gastrodin and gastrodin in the gastrodia elata extract is increased, so that the drug effect is improved. The preparation process is green and environment-friendly, high selectivity is achieved, large-scale production can be achieved, the extraction efficiency and purity are improved, and the cost is reduced.

The invention discloses a preparation method and application of a bletilla striata component extract for improving diabetic foot ulcer pain, and the method comprises the following steps: S1, washing bletilla striata tubers with deionized water, air-drying, and crushing to obtain bletilla striata powder; s2, adding the porous magnetic microsphere immobilized cellulase into deionized water, performing ultrasonic dispersion, adding bletilla striata powder, and performing stirring reaction under ultrasonic assistance and heating; s3, filtering and collecting filtrate 1, adding filter residues into deionized water, stirring and reacting under ultrasonic assistance and heating, filtering and collecting filtrate 2, combining and collecting the filtrate 1 and the filtrate 2, and concentrating to obtain a crude bletilla striata extracting solution; and S4, adding ethanol into the crude bletilla striata extracting solution, stirring, standing overnight, centrifuging, washing precipitate with ethanol, re-dissolving in deionized water, stirring, filtering, ultra-filtering and concentrating the obtained solution, then adding ethanol, stirring, standing overnight, centrifuging, washing precipitate with ethanol, and drying to obtain the bletilla striata component extract.

The invention belongs to the technical field of biomass resource utilization, and discloses cardamine violifolia processing by-product-based charcoal immobilized cellulase as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing ferric chloride with the cardamine violifolia processing byproduct, and performing pyrolysis carbonization treatment to obtain a pyrolysis product; the pyrolysis product is subjected to acid pickling, water washing and drying treatment in sequence, and charcoal is obtained; and carrying out immobilization treatment on cellulase by taking the biochar as a carrier to obtain the cardamine violifolia processing byproduct-based biochar immobilized cellulase. The method provided by the invention not only realizes resource utilization of cardamine violifolia processing byproducts, but also provides a new path for preparation of immobilized cellulase with high loading capacity and high stability in the field of enzymatic preparation of glucose.

The invention provides a cervus elaphus linnaeus health-care wine for dispelling wind and clearing damp and a preparation method thereof, and belongs to the technical field of health-care products. Sequentially cleaning, airing, mixing, crushing and sieving hairy antler, radix angelicae pubescentis, radix saposhnikoviae, notopterygium root, herba taxilli, eucommia ulmoides, radix achyranthis bidentatae, astragalus membranaceus, mulberry, cassia twig and liquorice to obtain mixed powder, dispersing the mixed powder and immobilized cellulase into white spirit with the alcoholic strength of 5-10% vol, and soaking for 4-6 hours to obtain an enzymolysis system; transferring the enzymolysis system into Baijiu with the alcoholic strength of 50% vol, soaking for 15-20 days, and sequentially filtering, centrifuging and concentrating to obtain a concentrated solution; mixing rock sugar, honey and purified water to obtain a sugar solution, mixing the concentrated solution, the sugar solution, 60% vol fen-flavor base wine and purified water, and blending the alcoholic strength to 35% vol to obtain a mixed solution; and sequentially aging, filtering and sterilizing the mixed solution to obtain the wind-dispelling and dampness-eliminating cervus elaphus linnaeus health-care wine. And the immobilized cellulase is adopted to carry out enzymolysis treatment on the mixed powder, so that the preparation time is shortened, and the preparation efficiency is improved.