5 results about "Immobilized cellulase" patented technology

The invention discloses a method for immobilizing cellulase by using a Ti3O5-based composite photothermal conversion material and application. The material is composed of a Ti3O5 core coated with polydopamine and a glycidyl methacrylate modified azobenzene polymer brush grafted on the surface of the Ti3O5 core. The preparation method comprises the following steps: forming a PDA layer on the surface of Ti3O5 through dopamine auto-polymerization, synthesizing a gAZO polymer brush, and finally modifying the gAZO onto the PDA-coated Ti3O5 through photo-initiation grafting reaction. The material disclosed by the invention can realize local temperature regulation and control at 35-50 DEG C under the irradiation of visible light of 100-150 mW / cm; when being used for immobilizing cellulase, the immobilized cellulase can be locally heated through illumination in the synchronous saccharification and fermentation process, so that the problem that the optimal saccharification and fermentation temperatures are not matched is solved, and the SSF efficiency is improved; the process is simple, and a novel controllable temperature control strategy is provided for the bio-refinery process.

The invention discloses a rhizoma polygonati polysaccharide extraction method, and belongs to the technical field of rhizoma polygonati polysaccharide extraction, the method comprises the following steps: S1, preparing Fe3O4-chitosan magnetic nanoparticles, and jointly immobilizing cellulase and pectinase on the Fe3O4-chitosan magnetic nanoparticles to obtain Fe3O4-chitosan magnetic nanoparticles co-immobilized by the cellulase and the pectinase; the preparation method comprises the following steps: dispersing Fe3O4-chitosan magnetic nanoparticles co-immobilized by cellulase and pectinase in a protective agent solution, uniformly stirring, adding a proper amount of cross-linking agent glutaraldehyde, reacting at room temperature for 2-4 hours to form a first layer of protective film, and repeating the steps to form a plurality of layers of protective films (generally 2-3 layers); and the Fe3O4-chitosan magnetic nanoparticles which are co-immobilized by the cellulase and the pectinase and coated by the protective agent are obtained. The extraction rate and the purity of the polygonatum polysaccharide are improved, and meanwhile the content of impurities is reduced.

The application belongs to the technical field of enzyme engineering and new material cross, and particularly relates to a kind of immobilized cellulase of magnetic mesoporous core-shell material and preparation method and application. The immobilized cellulase is obtained by physical adsorption of cellulase with magnetic mesoporous core-shell material F5HPU-46 as carrier. F5HPU-46 is a unique core-shell structure with paramagnetic Fe3O4 nanoparticles as core layer and UIO-66 mesoporous shell layer with stable structure, which has the dual advantages of magnetic response and high specific surface area mesoporous structure. The relative enzyme activity of the immobilized cellulase in the application is increased to 119% under acidic conditions at pH 4, while the relative enzyme activity of free enzyme is decreased to 75%.

The present application relates to papermaking auxiliary technical field, specifically to a kind of papermaking with fibrous property improved biological enzyme composition and preparation method thereof, comprising: immobilized xylanase 10 parts by weight-40 parts by weight, enzyme activity 1000U / g-1500U / g;Immobilized cellulase 5 parts by weight-25 parts by weight, enzyme activity 700U / g-1000U / g;Immobilized pectinase 6 parts by weight-35 parts by weight, enzyme activity 500U / g-800U / g;Cassava starch and diatomite compound carrier 5 parts by weight-20 parts by weight, wherein the mass ratio of cassava starch and diatomite is (2-5):1;Composite stabilizer 0.5 parts by weight-8 parts by weight, which is composed of trehalose and propylene glycol according to mass ratio (0.5-2):1;Chitosan auxiliary enzyme agent 1 parts by weight-5 parts by weight;And deionized water.The present application solves the problems of insufficient stability, single modification effect and limited application range of existing papermaking biological enzyme preparation by multi-enzyme synergistic compounding, differential immobilization, optimized combination of compound carrier and synergistic adjuvant, and has outstanding application advantages.

The invention discloses a preparation method for synthesizing gastrodin and gastrodin through double-enzyme catalysis and application of the gastrodin and the gastrodin, and belongs to the technical field of compound extraction. The technical problems to be solved are that the chemical synthesis process of gastrodin and gastrodigenin is relatively high in safety risk, more in toxic and harmful reagent solvent residue, easy to generate by-products, limited in yield and low in purity, time-consuming and labor-consuming in extraction and separation from traditional Chinese medicinal materials, long in extraction time, low in extraction yield and purity, and low in effective content in medicines. According to the technical scheme, the method is characterized in that immobilized cellulase and lipase are adopted as biocatalysts to catalyze parishin compounds in the gastrodia elata extract to be converted into gastrodin and gastrodin, and the content of gastrodin and gastrodin in the gastrodia elata extract is increased, so that the drug effect is improved. The preparation process is green and environment-friendly, high selectivity is achieved, large-scale production can be achieved, the extraction efficiency and purity are improved, and the cost is reduced.