Method for extracting wheat germ oil by immobilized enzyme method

A technology of wheat germ oil and immobilized enzymes, which is applied in the direction of fat oil/fat production and fat production, and can solve problems such as low oil yield, waste of resources, and toxic solvents

Inactive Publication Date: 2016-11-23
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional pressing method has the disadvantages of low oil yield, great destruction of nutrients, and serious waste of resources. The crude oil obtained by the leaching method has more non-oily substances, poor quality, and the solvent used is toxic.
At present, there are few related studies on the extraction of wheat germ oil by aqueous enzymatic method, and it can be predicted that the extraction of wheat germ oil by aqueous enzymatic method has considerable development prospects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: The 6 k carbon fibers were chopped into 0.5 cm lengths, and then pretreated: soaked in acetone for 6 h, filtered to recover the supernatant, washed with 4 times the volume of carbon fiber distilled water, and then kept the volume of distilled water greater than 2 times the volume of carbon fibers, and in Treat in a boiling water bath for 3 hours, take out the carbon fiber, rinse it with distilled water three times the volume of the carbon fiber, and put it in a bellows for drying. Then add 10 g / L into a 250 mL shake flask containing 50 mL deionized water, shake at 180 rpm for 6 h, and finally take out the pelleted carbon fibers and dry them for later use.

[0024] Put 50 g of raw wheat germ into the microwave turntable according to the material thickness of 1 cm, and then heat it in the microwave oven for 90 s, then take out the wheat germ, and grind the treated wheat germ into fine particles. After the wheat germ is ground, it is sieved with a 40-mesh stan...

Embodiment

[0028] Example 2: Compared with Example 1, the difference of this example is that the 3 k carbon fibers are chopped into lengths of 1.5 cm, and then pretreated: after soaking in acetone for 6 h, put 3 times the volume of deionized water into a boiling water bath for treatment After 4 h, take out the carbon fiber, rinse it with distilled water twice the volume of the carbon fiber, and put it in a bellows to dry for later use.

[0029] Put 50 g of raw wheat germ into the turntable of the microwave oven according to the material thickness of 1 cm, and then heat it in the microwave oven for 100 s, then take out the wheat germ, and grind the treated wheat germ into fine particles. After the wheat germ is ground, it is sieved with a 60-mesh standard sieve to obtain smaller granular wheat germ. Then use the vegetable oil extraction solvent as the extraction reagent, add pH=4.8, 0.05 mol / L citrate buffer according to the ratio of solid to liquid of 2:5 and stir to obtain the slurry....

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PUM

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Abstract

The invention belongs to the technical field of oil extraction, and in particular relates to a method for extracting wheat germ oil by immobilized enzyme method. The method comprises the following steps: pretreatment of enzyme immobilization carrier carbon fiber and raw material wheat germ, crushing of wheat germs and then sieving with a 40 to 100 mesh screen, adjustment of material-liquid ratio and solution ionic strength, use of carbon fiber carrier immobilized cellulase and amylase for enzymolysis treatment of the raw material, centrifugal separation of enzymolysis liquid of wheat enzymolysis to obtain the wheat germ oil, and detection of oil extraction rate. Through use of carbon fiber immobilized bienzyme and water enzyme method for extraction of the wheat germ oil, nutrients in the raw material are not destroyed, the obtained wheat germ oil is clear and transparent, and has good quality, and the oil extraction rate is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of oil extraction, and in particular relates to a method for extracting wheat germ oil by an immobilized enzyme method. Background technique [0002] Wheat is the main crop in my country, and a large amount of wheat germ will remain in flour production. Wheat germ is an important part of wheat grain, rich in a large amount of high-quality protein, fat and vitamins. Wheat germ oil is a kind of vegetable oil extracted from wheat germ as raw material, and the unsaturated fat, linoleic acid and vitamin E contained in it are one of the important sources of nutrition. It has obvious biological effects on regulating human blood pressure, reducing the concentration of low-density lipoprotein and total cholesterol in the blood, and thus has the function of preventing cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease and hypertension. Therefore, the development and utilizati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 王乐秦昂武大鹏王金水
Owner HENAN UNIVERSITY OF TECHNOLOGY
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