Method for improving enzymatic activity and utilization rate of immobilized enzymes in constant magnetic filed three-phase fluidized bed

A technology of immobilized enzyme activity and three-phase fluidized bed, applied in the direction of immobilized enzymes, biochemical equipment and methods, enzymes, etc., can solve the difficulties of enzyme recovery, low enzyme activity of immobilized enzymes, and low enzyme reuse rate and other problems, to achieve the effect of improving enzyme activity and utilization rate, good stability, and improving enzyme activity and utilization rate
CN101696440AInactive Publication Date: 2010-04-21SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2010-04-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for improving enzymatic activity and utilization rate of immobilized enzymes in a constant magnetic filed three-phase fluidized bed. The method can effectively improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes by applying a constant magnetic field to a magnetic field three-phase fluidized bed and regulating and controlling various parameters of the constant magnetic field, such as magnetic field strength, magnetic field action time, gas velocity, fluid velocity and the like. The magnetic immobilized cellulolytic enzymes used by the method of the invention have excellent stability and can be repeatedly used for 9 times after being recycled, and the recovery ratio of the enzymatic activity is over 42 percent. The method can improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes so as to effectively reduce cost and simultaneously can improve the degradation rate of macromolecular substances and provides the basis for realizing continuous production.
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Description

technical field

[0001] The invention relates to a method for improving the enzyme activity and utilization rate of magnetically immobilized enzymes, in particular to a method for improving the enzyme activity and utilization rate of immobilized enzymes in a constant magnetic field three-phase fluidized bed. Background technique

[0002] At present, the degradation methods of macromolecular high-viscosity substances (such as natural chitosan macromolecular systems) mainly include: chemical methods, physical methods, and enzymatic hydrolysis methods. Because enzymatic degradation has the advantages of specificity, selectivity, easy control, and basically no pollution to the environment, enzymatic hydrolysis is an ideal method.

[0003] In immobilized enzyme engineering, small particles are often used as immobilized enzyme carriers because of their high immobilized specific surface area. However, for the common immobilized enzyme reactor—fixed bed reactor, the small particle c...

Claims

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