Preparation method of sustained-release immobilized cellulase for feeding

A cellulase and sustained-release technology, which is applied in the direction of being fixed on/in an organic carrier, can solve the problems of easy contamination and miscellaneous bacteria, high cost, poor controllability, etc. Effect

Inactive Publication Date: 2016-01-06
梅庆波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: for the production of cellulase by solid fermentation method, it occupies a large area, is easy to be contaminated with bacteria, and has poor production stability, and the technology is not easy to master, resulting in low enzyme activity, low yield, and high cost. In view of the disadvantages of poor controllability, a preparation method of sustained-release immobilized cellulase for feed is provided. The present invention mixes gelatin with deionized water, glucose aqueous solution and polyethylene glycol aqueous solution, and then mixes it with glycerin, glacial acetic acid solution, The cross-linking agent is mixed into a slow-release coating material for subsequent use; then mannitol, tryptone, yeast and agar are mixed to adjust the pH value to prepare a culture medium for Clostridium perfringens, which is inoculated with Clostridium perfringens for cultivation. Clostridium perfringens is inoculated in the vegetable bran cake glucose solution prepared from vegetable bran cake. After sealed culture, it is opened to let in air, evaporated to dryness, mixed with the spare slow-release coating material in a mold, pressed, and dried. have to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First, according to the mass ratio of 1:30, gelatin is added into deionized water for dissolving, and after it is heated to 50°C, it is stirred at 1200r / min to form a gelatin solution, and then the above-mentioned 30 parts of gelatin solutions are respectively selected according to parts by weight , 30 parts of 0.6mol / L glucose aqueous solution and 40 parts of 0.9mol / L polyethylene glycol aqueous solution were mixed, the temperature was raised to 50°C, and the gelatin mixture was prepared with stirring for 5 minutes; then after the stirring was completed, press In terms of parts by weight, select 30 parts of gelatin mixture, 30 parts of glycerin and 40 parts of glacial acetic acid solution, slowly stir and mix, and the mixing speed is 300r / min. After the above solution is mixed, mix the crosslinking agent with a mass ratio of 1:25 Add it to the above mixed solution to make it cross-linked to form a sustained-release coating material, ready for use, the cross-linking agen...

example 2

[0019]First, according to the mass ratio of 1:30, gelatin is added into deionized water for dissolving, after it is heated to 55°C, at 1350r / min, stirred to form a gelatin solution, and then the above-mentioned 35 parts of gelatin solutions are respectively selected according to parts by weight , 20 parts of 0.6mol / L glucose aqueous solution and 45 parts of 0.9mol / L polyethylene glycol aqueous solution were mixed, the temperature was raised to 55°C, and the mixture was kept and stirred for 8 minutes to prepare the gelatin mixture; then after the stirring was completed, press In terms of parts by weight, select 50 parts of gelatin mixture, 25 parts of glycerin and 25 parts of glacial acetic acid solution, slowly stir and mix, the mixing speed is 400r / min, after the above solution is mixed, press the mass ratio of 1:25, mix the crosslinking agent Add it to the above mixed solution to make it cross-linked to form a sustained-release coating material, ready for use, the cross-linki...

example 3

[0022] First, according to the mass ratio of 1:30, gelatin is added into deionized water for dissolving, and after it is heated to 60°C, under 1500r / min, stirring forms a gelatin solution, and then the above-mentioned 40 parts of gelatin solutions are respectively selected according to parts by weight , 20 parts of 0.6mol / L glucose aqueous solution and 40 parts of 0.9mol / L polyethylene glycol aqueous solution were mixed, the temperature was raised to 60°C, and the mixture was kept and stirred for 10 minutes to prepare the gelatin mixture; then after the stirring was completed, press In terms of parts by weight, select 60 parts of gelatin mixture, 20 parts of glycerin and 20 parts of glacial acetic acid solution, slowly stir and mix, and the mixing speed is 500r / min. After the above solution is mixed, mix the crosslinking agent with a mass ratio of 1:25 Add it to the above mixed solution to make it cross-linked to form a sustained-release coating material, ready for use, the cro...

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PUM

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Abstract

The invention discloses a preparation method of sustained-release immobilized cellulase for feeding, which belongs to the field of cellulase preparation. The method comprises the following steps: taking gelatin, mixing the gelatin with deionized water, a glucose aqueous solution and a polyethylene glycol aqueous solution, and mixing the obtained mixture with glycerin, a glacial acetic acid solution and a crosslinking agent so as to obtain a sustained-release coating material for standby application; and taking and mixing mannitol, tryptone, yeast and agar, adjusting the pH value of the obtained mixture so as to obtain a clostridium perfringens culture medium, inoculating the clostridium perfringens culture medium, inoculating clostridium perfringens in a rapeseed bran cake so as to obtain a rapeseed bran cake-glucose solution, after the clostridium perfringens are subjected to sealed culture, opening the culture medium for feeding air, evaporating clostridium perfringens containing rapeseed bran cake particles until the particles are dry, mixing the dry particles with the standby sustained-release coating material, pressing the obtained mixture in a die, and drying the obtained product, so that the sustained-release immobilized cellulase is obtained. The method disclosed by the invention has the beneficial effects that the preparation cost is reduced by 18% in comparison with traditional methods, the production efficiency is high, the enzyme activity of the cellulase is increased by 42%, and the enzymatic activity is greater than 9250 IU / g.

Description

technical field [0001] The invention relates to a preparation method of sustained-release immobilized cellulase for feed, and belongs to the field of cellulase preparation. Background technique [0002] Cellulase (β-1,4-glucan-4-glucan hydrolase) is a general term for a group of enzymes that degrade cellulose to generate glucose. It is not a monomeric enzyme, but a synergistic multi-component The enzyme system is a complex enzyme, mainly composed of exo-β-glucanase, endo-β-glucanase, and β-glucosidase, as well as a highly active xylanase, which acts on Cellulose and products derived from cellulose. Microbial cellulase is of great significance in converting insoluble cellulose into glucose and destroying cell walls in fruit and vegetable juices to improve juice yield. Cellulase is widely found in organisms in nature. Bacteria, fungi, and animals can produce cellulase. The production of bacterial cellulase is less, mainly endoglucanase, most of which have no degradative act...

Claims

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Application Information

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IPC IPC(8): C12N11/08
Inventor 梅庆波高力群盛艳花
Owner 梅庆波
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