Preparation method of nano immobilized cellulase

A cellulase and cellulose technology, which is applied in the direction of being fixed on or in an inorganic carrier, can solve the problems of low enzymatic activity retention rate, incapable of industrialized production, complicated preparation process, etc., and achieves a simple and environmentally friendly method for repeated use. , the effect of compensating for production costs
CN103397014AInactive Publication Date: 2013-11-20
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Patent Information

Authority / Receiving Office
CN · China
Publication Date
2013-11-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of biochemical engineering, and discloses a preparation method of nano immobilized cellulase, which comprises the following steps: adding a HCl solution into ethyl orthosilicate while stirring, and hydrolyzing to obtain a transparent solution; cooling the transparent solution, recovering ethanol, and controlling the ethanol volume percent of the transparent solution to be less than 1%; adding a glucose solution into the transparent solution while stirring, then adding a cellulase citric acid-sodium citrate buffer solution, finally regulating the pH value to approximate neutral, transferring to 4-10 DEG C, and gelatinizing; and grinding the wet gel obtained after gelatinizing, washing with water to remove the glucose, performing vacuum filtration on the mixed solution after water washing to obtain a white gel, drying the gel, grinding, and screening for later use. Experiment shows that the enzyme activity is almost kept unchanged after the nano immobilized cellulase prepared by the method is repeatedly used multiple times; and the method can be used for large-scale production of cellulosic ethanol.
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Description

technical field

[0001] The invention relates to the field of biochemical industry, in particular to a preparation method of nano-immobilized cellulase. Background technique

[0002] With rising international crude oil prices and worsening global pollution problems, countries all over the world are sparing no effort to develop new energy sources, and bioethanol is a new type of fuel that is generally favored. In the 21st century, the use of cellulase to convert cellulose to produce glucose and then ferment to obtain bioethanol can avoid a large loss of food crops, which has attracted the attention of governments and research institutions in various countries. The key to this is the cost of cellulase .

[0003] Due to the low fermentation activity of cellulase, its application cost is also high. Compared with other glycoside hydrolases, the specific activity of cellulase is at least 1 to 2 orders of magnitude lower, resulting in lower enzyme efficiency. In addition, because...

Claims

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