49results about How to "Achieve commercial production" patented technology

This invention relates to a method and system of preparing ethanol based on the solid state fermentation of sweet sorghum stem, belonging to the ethanol production technology of solid state fermentation. It includes the following key processes and device: raw materials smash is to choose the suitable mill to smash sweet sorghum stalk, strain addition is to add ethanol bacteria at the entrance of solid-state fermentation tank, solid-state fermentation is that the raw materials added bacteria is fermented in the slant rotor solid-state fermentation tank, solid distillation is to distill the solid-state fermented materials to obtain ethanol.

The invention discloses a morchella esculenta cultivating strain and an inducting method and a cultivating method therefor. An inducting culture medium of a morchella esculenta cultivating strain mother strain is used to induce morchella esculenta; a cultivating culture medium of a morchella esculenta cultivating strain is used to cultivate the morchella esculenta; the inducing method comprises the steps of taking out a preserved test tube strain; inoculating the test tube strain to an inducting culture medium flat plate; culturing in a culture box at a temperature of 18-22 DEG C; after hyphae grow on the whole plate, taking the hyphae as inoculating hyphae to be placed in the cultivating culture medium for use; and the cultivating method comprises the steps of sterilizing, inoculating and performing management on two stages of a mycelium growth period and a sporocarp growth period. The invention, through long-term test, searching and discussion, provides a morchella esculenta artificial cultivating technological method which has breakthrough at home and abroad; the morchella esculenta cultivating strain is an optimized and induced morchella esculenta strain; an integrated technology capable of maintaining stable production and continuous production is achieved; and commercial production is realized.

The invention belongs to the technical field of natural polymer materials and particularly discloses bi-network high-intensity fibroin hydrogel and a preparation method thereof. Fibroin hydrogel subjected to chemical crosslinking (a first network) of horse radish peroxidase is soaked in a solution which can induce fibroin beta to fold and transform, physical crosslinking (a second network) is formed, and then the bi-network high-intensity fibroin hydrogel with the solid content being 2-20 wt% can be prepared. The hydrogel is good in elasticity, high in intensity and easy to regulate and has biocompatibility, the performance is stable under the condition of the simulated in-vivo environment, and the bi-network high-intensity fibroin hydrogel can be widely used for fields of drug sustained release carriers, cartilage tissue, ligament tissue and other biomedicine and regeneration medicine engineering.

The invention provides milk goat fermented total mixed ration; after fresh green forage with high water content is mixed with other milk goat feed components, the anaerobic fermentation is implemented to prepare the milk goat fermented total mixed ration, wherein the mass ratio of the fresh green forage with high water content to the other milk goat feed components ensures that the total water content is 40-50% after mixing. The milk goat fermented total mixed ration uses the fresh green forage as the main raw materials to be reasonably matched with such components as energy feed, protein feed and feed additive premix; the proper water content is controlled through regulating the matching ratio of feeds; then the anaerobic fermentation is implemented to prepare the total mixed ration product. The FTMR (Fermented Total Mixed Ration) restrains the breeding of microorganisms mainly through causing the physiologic dry of the microorganisms and the anaerobic fermentation, so that the storage resistance of the FTMR can be improved, the storage time is prolonged, the source of the green forage is widened, the fermentation degree of the high-quality green forage is prominently reduced, and the nutritional value of the green forage is effectively preserved.

The invention discloses artificial marble polishing waste residue-based ceramic glazed tiles which are prepared from the following main components in percentage by weight: 1 to 25 percent of artificial marble polishing waste residues, 1 to 25 percent of wollastonite, 50 to 70 percent of feldspar and 15 to 25 percent of black mud. The ceramic glazed tiles are prepared from the artificial marble polishing waste residues serving as the raw materials; long-term pollution to the environment due to the artificial marble polishing waste residues treated in a landfill treatment manner can be completely avoided; meanwhile, by the use of the characteristic that the CaCO3 content in the artificial marble polishing waste residues is high, the artificial marble polishing waste residues can completely replace conventional limestone in the formula of ceramic glazed tile green bodies, so that the cost of the formula can be reduced, and wastes can be turned into treasures. The invention further discloses a preparation method of the artificial marble polishing waste residue-based ceramic glazed tiles. The preparation method is simple in flow and convenient to operate, can realize commercial production, and has an extremely application prospect.

The invention relates to a method for producing pediococcusacidilactici by accumulation, comprising the following steps of: inoculating the pediococcusacidilactici in a modified MRS liquid nutrient medium, culturing at 30DEG C for 8 hours, then inoculating the pediococcusacidilactici with the inoculum concentration of 4wt% into a fermentation tank, performing batch fermentation at the first stage of fermentation, performing the second stage of fermentation when the concentration of residual sugar (residual glucose) in the fermentation liquid is below 5-10g/L, and fermenting for 16-30 hours to obtain pediococcusacidilactici with the valence of 18000-24000AU/ml. Compared with the prior art, the method provided by the invention has a short fermentation period and simplicity of operation, and is favorable to quick production of pediococcusacidilactici, and the highest valence of the obtained product is 24000AU/ml.

The invention relates to a method for producing mycophenolic acid from penicillium brevicompactum by high-efficiency accumulation. The method comprises the following steps of: inoculating 2wt% of penicillium brevicompactum in content onto a potato dextrose agar (PDA) inclined plane, controlling the temperature at 28DEG C, activating and culturing for 72hours; and then, inoculating the activated penicillium brevicompactum into a fermentation tank, fermenting and culturing by an optimized glucose-glycine liquid nutrient medium, controlling the temperature at 28DEG C, and fermenting to obtain mycophenolic acid. Compared with the prior art, the fermentation period of the method provided by the invention is within 240 hours, and the maximum yield can achieve 5.0g/L which is improved by about 30% compared with batch fermentation. The fermentation strategy of optimizing the carbon-nitrogen ratio, supplementing feed, adjusting the pH by sections and feeding back feed supplement is simple to control, has higher yield of mycophenolic acid, and has wider industrialized application prospect.

The invention discloses a preparation method of deuterated pimozide. The pimozide-d4 is synthesized from 4-bromopentafluorobenzene-d4 through eight steps. The provided preparation method has the advantages of reasonable technological design, simple operation, easy product separation and purification, easily-available raw materials, high yield, and high purity. The isotope abundance of the obtained target product is high. The prepared stable isotope-labeled pimozide-d4 can be well applied to clinical pharmacokinetics researches, so people can acknowledge the metabolism process and action mechanism of pimozide in human body more precisely and conveniently, and thus the deuterated pimozide has an important application value.

The invention provides a GAT vector for mediating and regulating male fertility of a plant recessive genic male sterile line and application of the GAT vector. The GAT vector comprises five functional element expression cassettes: a plant male fertility restoration gene element expression cassette used for restoring male fertility of recessive genic male sterility mutants, a plant pollen abortion gene element expression cassette used for removing pollen containing GAT and keeping a heterozygous state of a GAT maintainer line, a chemical herbicide positive selection expression cassette used for gene transformation and GAT maintainer line impurity removal and purification, a chemical herbicide negative selection expression cassette used for removing herbicide-sensitive GAT maintainer line pollen and seed escape and removing impurities and purifying a GAT sterile line; and a seed screening element expression cassette used for mechanically sorting seeds. The GAT vector disclosed by the invention can be used for crossbreeding and hybrid seed production of plant recessive genic male sterility materials, so that new plant varieties with high quality, high yield, wide applicability and high resistance and seeds of the new plant varieties are obtained, and the GAT vector has huge economic value.

The invention discloses a teriparatide freeze-dried pharmaceutical composition and a preparation method thereof, and the teriparatide freeze-dried pharmaceutical composition comprises the following raw materials in parts by weight: 0.2 part of teriparatide, 50-150 parts of mannitol, 5-50 parts of lactose, 0.1-1 part of menthol, 0.1-0.5 part of sodium acetate, 0.1-0.5 part of glacial acetic acid, and a proper amount of a pH regulator for regulating the pH value to 3.0-6.0. The administration mode of the teriparatide freeze-dried pharmaceutical composition is sublingual administration, the formula of the teriparatide freeze-dried pharmaceutical composition provided by the invention does not contain a bacteriostatic agent, so that the toxicity to a human body is less, in addition, the administration mode of the teriparatide freeze-dried pharmaceutical composition is sublingual administration, injection administration is not needed, and the application range is wide. The pricking feeling caused by administration is reduced, the sterile risk in clinical use is reduced, and the compliance of a patient is improved.