Method for extracting albumen powder from corn germ meal

A technology of corn germ meal and protein powder, which is applied in the field of protein engineering, can solve the problems of narrow application range and achieve the effects of wide application range, increased product added value, and simple process

Active Publication Date: 2015-03-04
SHANDONG XIWANG SUGAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is suitable for the production of corn germ protein from corn germ meal after steaming and frying to extract corn oil, but it is not suitable for corn germ meal after extracting corn oil, and its application range is narrow

Method used

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  • Method for extracting albumen powder from corn germ meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for extracting protein powder from corn germ meal, comprising the steps of:

[0036] (1) After 5kg of corn germ meal extracted from corn oil by steaming, frying, crushing and passing through a 200-mesh sieve, add 95kg of water, the weight ratio of the amount of water added to corn germ meal is 95:5, stir evenly to make a suspension, Pasteurization at 85°C to obtain the sterilized corn germ meal suspension;

[0037](2) Cool down the sterilized corn germ meal suspension obtained in step (1) naturally, and when the suspension is stable to 40°C, adjust the pH of the solution to 8.0-8.5 with 0.1mol / l NaOH solution;

[0038] (3) Add 0.04kg of compound protease, the added compound protease is a mixture of alkaline protease Alacalase-2.40.01kg and Protamex protease 0.03kg; keep stirring slowly at 55°C at a stirring speed of 12 rpm, maintain for 4h, carry out Enzymolysis to obtain enzymatic solution;

[0039] (4) Add alkaline protease Alacalase-2.4 solution to the enz...

Embodiment 2

[0045] A method for extracting protein powder from corn germ meal, comprising the steps of:

[0046] (1) After extracting corn oil, 5kg of corn germ meal was crushed and passed through a 200-mesh sieve, and then 95kg of water was added. The weight ratio of water to corn germ meal was 95:5. Sterilized to obtain the sterilized corn germ meal suspension liquid;

[0047] (2) Cool down the sterilized corn germ meal suspension obtained in step (1) naturally, and when the suspension is stable to 40°C, adjust the pH of the solution to 8.0-8.5 with 0.1mol / l NaOH solution;

[0048] (3) Add 0.04kg of compound protease, the added compound protease is a mixture of alkaline protease Alacalase-2.40.016kg and Protamex protease 0.024kg; keep stirring slowly at 40°C, the stirring speed is 10 rpm, maintain for 4h, carry out Enzymolysis to obtain enzymatic solution;

[0049] (4) Add alkaline protease Alacalase-2.4 solution to the enzymolysis solution obtained in step (3), and add 1.8 μL per 10 ...

Embodiment 3

[0055] A method for extracting protein powder from corn germ meal, comprising the steps of:

[0056] (1) Mix 2.5kg of corn germ meal after steaming, frying and pressing to extract corn oil and 2.5kg of corn germ meal after extracting corn oil, after crushing and passing through a 200-mesh sieve, add 95kg of water, the amount of water added is the same as that of corn germ meal The weight ratio is 95:5, stirred evenly to become a suspension, pasteurized at 85°C to obtain a sterilized corn germ meal suspension;

[0057] (2) Cool down the sterilized corn germ meal suspension obtained in step (1) naturally, and when the suspension is stable to 40°C, adjust the pH of the solution to 8.0-8.5 with 0.1mol / l NaOH solution;

[0058] (3) Add 0.04kg of compound protease, the added compound protease is a mixture of alkaline protease Alacalase-2.40.01kg and Protamex protease 0.03kg; keep stirring slowly at 60°C, stirring speed is 12 rpm, maintain for 4h, carry out Enzymolysis to obtain enz...

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Abstract

The invention belongs to the technical field of protein engineering, and particularly relates to a method for extracting albumen powder from corn germ meal. The method comprises the following steps: firstly, crushing the corn germ meal, then adding water into the crushed corn germ meal, uniformly stirring the water and the corn germ meal till the turbid liquid is formed, and performing pasteurization at 85 DEG C; secondly, naturally cooling the turbid liquid, adjusting the pH value of the turbid liquid to 8.0 to 8.5 through a 0.1mol / l NaOH solution when the temperature of the turbid liquid is stabilized at 40 DEG C; thirdly, adding compound protease to perform enzymolysis to obtain the enzymatic hydrolysate; fourthly, adding Alacalase-2.4 liquid and papain into the enzymatic hydrolysate obtained in the third step to perform enzymolysis continuously, performing enzyme deactivation and centrifugal separation, taking the liquid supernatant, heating the liquid supernatant to evaporate moisture, and obtaining the albumen powder product after drying. Through the adoption of the method for extracting albumen powder from corn germ meal, both corn germ protein which is denatured at high temperature and corn germ protein which is not denatured can be used for extracting albumen powder. The method is wide in application scope, and various corn germ materials can be adopted.

Description

technical field [0001] The invention belongs to the technical field of protein engineering, and in particular relates to a method for extracting protein powder from corn germ meal. Background technique [0002] Corn germ is a by-product of corn starch processing. It is rich in domestic sources and contains high fat and protein. Currently it is generally used to produce corn germ oil. In order to increase the oil yield of the germ, the germ is traditionally subjected to heat treatment above 100°C (also known as steaming and frying), the purpose is to destroy the cell wall of the germ, make the protein coagulate and denature, reduce the viscosity of the oil, and make it coagulate and flow out. But only considered the recovery of 35-40% oil in the germ, did not consider that the germ protein that still contains about 20% in the germ cannot be recycled. Corn germ protein is a high-quality protein, mainly composed of globulin and albumin, which account for more than 60% of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/12
Inventor 王棣吉晓兵陶兴发崔强李昕李秋红李曰强毕景辉葛金华王非薛梅
Owner SHANDONG XIWANG SUGAR
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