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Method for extracting albumen powder from corn germ meal

A technology of corn germ meal and protein powder, which is applied in the field of protein engineering, can solve the problems of narrow application range and achieve the effects of wide application range, low energy consumption and simple process

Active Publication Date: 2014-01-15
SHANDONG XIWANG SUGAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is suitable for the production of corn germ protein from corn germ meal after steaming and frying to extract corn oil, but it is not suitable for corn germ meal after extracting corn oil, and its application range is narrow

Method used

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  • Method for extracting albumen powder from corn germ meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for extracting protein powder from corn germ meal includes the following steps:

[0036] (1) After steaming, frying, squeezing and extracting corn oil, 5 kg of corn germ meal is crushed and passed through a 200-mesh sieve. 95kg of water is added. The weight ratio of water to corn germ meal is 95:5. Stir evenly to make it a suspension. Pasteurized at 85°C to obtain a sterilized corn germ meal suspension;

[0037] (2) The sterilized corn germ meal suspension obtained in step (1) is naturally cooled, and when the suspension is stable to 40°C, the pH of the solution is adjusted to 8.0-8.5 with a 0.1 mol / l NaOH solution;

[0038] (3) Add 0.04kg composite protease, the added composite protease is a mixture of alkaline protease Alacalase-2.40.01kg and Protamex protease 0.03kg; stir slowly at a temperature of 55°C at a stirring speed of 12 revolutions / min and maintain for 4 hours. Enzymolysis to obtain enzymolysis solution;

[0039] (4) Add the alkaline protease Alacalase-2.4...

Embodiment 2

[0045] A method for extracting protein powder from corn germ meal includes the following steps:

[0046] (1) After extracting corn oil, 5 kg of corn germ meal is crushed and passed through a 200-mesh sieve. Add 95 kg of water. The weight ratio of the added water to the corn germ meal is 95:5. Stir evenly to make it into a suspension at 85°C. Sterilize to obtain the corn germ meal suspension after disinfection;

[0047] (2) The sterilized corn germ meal suspension obtained in step (1) is naturally cooled, and when the suspension is stable to 40°C, the pH of the solution is adjusted to 8.0-8.5 with a 0.1 mol / l NaOH solution;

[0048] (3) Add 0.04kg of composite protease, the added composite protease is a mixture of alkaline protease Alacalase-2.40.016kg and Protamex protease 0.024kg; stir slowly at a temperature of 40°C, stirring at a speed of 10 revolutions / min, and keep it for 4 hours. Enzymolysis to obtain enzymolysis solution;

[0049] (4) Add the alkaline protease Alacalase-2.4 so...

Embodiment 3

[0055] A method for extracting protein powder from corn germ meal includes the following steps:

[0056] (1) Mix 2.5 kg of corn germ meal after steaming, frying, squeezing and extracting corn oil and 2.5 kg of corn germ meal after extracting corn oil. After crushing and passing through a 200-mesh sieve, add 95kg of water. The weight ratio is 95:5, stir evenly to make a suspension, and pasteurize at 85°C to obtain a sterilized corn germ meal suspension;

[0057] (2) The sterilized corn germ meal suspension obtained in step (1) is naturally cooled, and when the suspension is stable to 40°C, the pH of the solution is adjusted to 8.0-8.5 with a 0.1 mol / l NaOH solution;

[0058] (3) Add 0.04kg of complex protease, the added complex protease is a mixture of alkaline protease Alacalase-2.40.01kg and Protamex protease 0.03kg; stir slowly at 60°C, the stirring speed is 12 revolutions / min, keep for 4h, proceed Enzymolysis to obtain enzymolysis solution;

[0059] (4) Add the alkaline protease A...

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Abstract

The invention belongs to the technical field of protein engineering, and particularly relates to a method for extracting albumen powder from corn germ meal. The method comprises the following steps: firstly, crushing the corn germ meal, then adding water into the crushed corn germ meal, uniformly stirring the water and the corn germ meal till the turbid liquid is formed, and performing pasteurization at 85 DEG C; secondly, naturally cooling the turbid liquid, adjusting the pH value of the turbid liquid to 8.0 to 8.5 through a 0.1mol / l NaOH solution when the temperature of the turbid liquid is stabilized at 40 DEG C; thirdly, adding compound protease to perform enzymolysis to obtain the enzymatic hydrolysate; fourthly, adding Alacalase-2.4 liquid and papain into the enzymatic hydrolysate obtained in the third step to perform enzymolysis continuously, performing enzyme deactivation and centrifugal separation, taking the liquid supernatant, heating the liquid supernatant to evaporate moisture, and obtaining the albumen powder product after drying. Through the adoption of the method for extracting albumen powder from corn germ meal, both corn germ protein which is denatured at high temperature and corn germ protein which is not denatured can be used for extracting albumen powder. The method is wide in application scope, and various corn germ materials can be adopted.

Description

Technical field [0001] The invention belongs to the technical field of protein engineering, and specifically relates to a method for extracting protein powder from corn germ meal. Background technique [0002] Corn germ is a by-product of corn starch processing. It is rich in domestic sources and contains high fat and protein. At present, it is generally used to produce corn germ oil. In order to increase the oil production rate of the germ, heat treatment (also known as steaming) above 100°C is traditionally applied to the germ to destroy the cell wall of the germ, coagulate and denature the protein, reduce the viscosity of the oil, and make it agglomerate and flow out. However, only the recovery of 35-40% of the oil in the germ is considered, and it is not considered that the germ protein still contains about 20% of the germ protein, which cannot be recycled. Corn germ protein is a high-quality protein, mainly composed of globulin and albumin, which account for more than 60% ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
Inventor 王棣吉晓兵陶兴发崔强李曰强毕景辉葛金华王非徐传娟
Owner SHANDONG XIWANG SUGAR
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