Preparation method of immobilized cellulase

Abstract

The invention discloses a preparation method of immobilized cellulase, relating to an enzyme immobilization method. The method comprises the following steps of: firstly, mixing chitosan with the degree of deacetylation of 90% with an acetic acid solution, then injecting the mixed solution into a 2% sodium tripolyphosphate solution, filtering and washing to obtain neutral spherical chitosan-polyethylene glycol carriers, then activating the carriers by a glutaraldehyde solution, carrying out immobilization reaction of the carriers and cellulase, and filtering and washing to obtain immobilized cellulase. The immobilized cellulase prepared by the method has the advantages of high mechanical strength, good enzyme stability, high enzyme activity, strong binding force between the enzyme and the carriers and high rate of immobilization.

Description

technical field

[0001] The invention relates to a biocatalyst immobilization method, more precisely a cellulase immobilization method, which belongs to the technical field of biological reagent preparation. Background technique

[0002] Natural cellulose is the most abundant renewable organic matter on the earth. The plant matter produced through photosynthesis in the world is about 1 trillion tons per year, 89% of which are not currently used by humans. From the perspective of human utilization of natural resources, this Undoubtedly a huge waste. With the rapid growth of the world's population and the unsolved food and energy crisis, the utilization of cellulose resources has aroused great concern and great attention from all countries in the world.

[0003] Ordinary organisms cannot directly use cellulose materials as food, and can only be used by hydrolyzing cellulose into glucose. The general methods for hydrolyzing cellulose into glucose include acid hydrolysis and en...