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Multi-arm magnetic graphene oxide composite microsphere, preparation method and application thereof

A technology of composite microspheres and oxidized stones, applied in the field of nanomaterials, can solve the problems of low immobilized amount of cellulase and poor activity of immobilized enzymes

Inactive Publication Date: 2018-08-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the current free cellulase immobilization amount is low, the activity of the immobilized enzyme synthesized is relatively poor, thereby synthesizing multi-arm magnetic graphene oxide composite microsphere immobilized cellulase

Method used

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  • Multi-arm magnetic graphene oxide composite microsphere, preparation method and application thereof
  • Multi-arm magnetic graphene oxide composite microsphere, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Preparation of multi-arm magnetic graphene oxide composite microspheres and immobilization of enzymes

[0065] (1) Preparation of graphene oxide (GO):

[0066] Accurately weigh 0.1g graphite powder and 0.1g sodium nitrate into a 100 mL beaker, slowly add 17 ml of sulfuric acid solution (12mol / L) to it, and place the beaker in an ice bath and stir for 15 min. Slowly add 2.0 g of potassium permanganate to the above solution, then transfer the suspension to a water bath with a temperature of 20°C, stir it magnetically for 90 minutes, and dilute the resulting brown paste with deionized water and stir for 10 minutes. Finally 4 mL of hydrogen peroxide was added, the reaction solution turned golden yellow, and the reaction was completed. After standing for 24 hours, the supernatant was removed, and the remaining precipitate was separated by suction filtration. The obtained product was washed with 5% hydrochloric acid several times until no white precipitate was detected...

Embodiment 2

[0077] Example 2: Preparation of multi-arm magnetic graphene oxide composite microspheres and immobilization of enzymes

[0078] (1) Preparation of graphene oxide (GO):

[0079] Accurately weigh 0.9 g of graphite powder and 0.9 g of sodium nitrate into a 100 mL beaker, slowly add 29 mL of sulfuric acid solution (12 mol / L) to it, and place the beaker in an ice bath and stir for 15 min. Slowly add 6.0 g of potassium permanganate to the above solution, then transfer the suspension to a water bath with a temperature of 60 ℃, and stir magnetically for 90 min. The resulting brown paste is diluted with deionized water and then stirred for 10 min. Add 8 mL of hydrogen peroxide, the reaction solution turns golden yellow, and the reaction is over. After standing for 24 hours, the supernatant was removed, and the remaining precipitate was separated by suction filtration. The obtained product was washed with 5% hydrochloric acid several times until no white precipitate was detected with bariu...

Embodiment 3

[0090] Example 3: Preparation of multi-arm magnetic graphene oxide composite microspheres and immobilization of enzymes

[0091] (1) Preparation of graphene oxide (GO):

[0092] Accurately weigh 0.5 g of graphite powder and 0.5 g of sodium nitrate into a 100 mL beaker, slowly add 23 mL of sulfuric acid solution (12 mol / L) to it, and place the beaker in an ice bath and stir for 15 min. Accurately weigh 4.0 g of potassium permanganate and slowly add it to the above solution, then transfer the suspension to a 40 ℃ water bath, and magnetically stir for 90 min. The resulting brown paste is diluted with 50 mL of deionized water and then stirred for 10 min. , And finally add 6 mL of hydrogen peroxide, the reaction solution turns golden yellow, the reaction is over. After standing for 24 hours, the supernatant was removed, and the remaining precipitate was separated by suction filtration. The obtained product was washed with 5% hydrochloric acid several times until no white precipitate wa...

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Abstract

Belonging to the technical field of nano materials, the invention relates to a multi-arm magnetic graphene oxide composite microsphere, a preparation method and application thereof. The method includes: subjecting GO@Fe3O4 and 4arm-PEG-NH2 to covalent binding to prepare GO@Fe3O4@4arm-PEG-NH2, then dissolving a certain amount of GO@Fe3O4@4arm-PEG-NH2 in a citrate buffer solution, then adding glutaraldehyde into the solution, and carrying out activating reaction for certain period of time; and washing the obtained product with the citrate buffer solution, and then conducting vacuum drying to obtain the material needed by the invention. The material obtained by the invention has superparamagnetism, the carry capacity of the composite microsphere applied to immobilized cellulase is increased to 575mg / g, and the obtained immobilized enzyme has the advantages of high temperature resistance, strong storage capacity and high reusability.

Description

Technical field [0001] The invention belongs to the technical field of nano materials, and relates to a multi-arm magnetic graphene oxide composite microsphere and a preparation method and application thereof; specifically, it relates to a nano material GO@Fe 3 O 4 @4arm-PEG-NH 2 Preparation method and application of immobilized cellulase. Background technique [0002] Cellulose is the most abundant renewable and biodegradable macromolecular polymer, which has been widely used in various industries. The annual output of fiber in nature is estimated to be 10 11 –10 12 Tons, although there are so many sources of cellulose, people’s utilization of this biomass resource is not very high. In many developing countries, it is used for cooking or heating in the form of combustion, which is a huge waste Resources destroy the environment. As a composite enzyme, cellulase can synergistically degrade cellulose, so it is widely used in the food industry, paper industry and bioenergy. Among t...

Claims

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Application Information

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IPC IPC(8): C12N11/08C12N11/14C12N9/42B01J13/02C01B32/198C12P19/14C12P19/02
Inventor 罗鹏韩娟王赟王丽李春梅倪良张文莉董剑
Owner JIANGSU UNIV
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