Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase

Abstract

This invention relates to fixed type cellulase polyvinyl alcohol nanometer super fine fibrous membrane prepn. method. It is composed of (by wt.%): 80-99 polyvinyl alcohol and 1-20 cellulase to form the membrane which thickness is 10-50 num with average 200 nm fiber. The procedures are as follows: polyvinyl alcohol with molecular wt. 60 thousands-100 thousands is dissolved in acetic acid buffer solution to form 5-8% solution, then proceeding static textile method to form a membrane, then proceeding valeric dialdehyde vapor crosslinking to produce the product. Said fixed type cellulase membrane material is used for degradation of chitin, with high biological activity of enzyme and it can be used for several times. Its production process is simple.

Description

technical field

[0001] The invention relates to a polyvinyl alcohol nano-ultrafine fiber film for immobilizing cellulase and a preparation method thereof, which belongs to the enzyme immobilization technology. Background technique

[0002] Electrospinning is a spinning process in which polymer solution or melt forms ultrafine fibers by means of high-voltage electrostatic action. Ultrafine fibers with a diameter of 3nm-1 μm can be prepared by electrospinning, and can be directly collected into ultrafine fiber membranes (Zheng M, Zhang Y Z, Kotaki M, Ramakrishna S.A review on polymernanofibers by electrospinning and their applications in nanocomposites. CompositesScience and Technology, 2003, 63, 2223-2253). Due to the characteristics of large specific surface area and high porosity, electrospun ultrafine fibers are used as carriers for immobilized enzymes, which is conducive to full contact between enzymes and substrates and reduces steric hindrance, so it has broad developm...