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A kind of growth-promoting composite microbial bacterial agent and its application

A technology of compound microbial inoculants and microbial strains, applied in the field of microorganisms, can solve the problems affecting the sustainable development of agriculture, poor water and fertilizer retention, poor permeability, etc. The effect of plant chlorophyll content

Active Publication Date: 2022-05-13
MINJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Problems such as poor permeability, poor water and fertilizer retention, and poor soil fertility in the current soil seriously affect the sustainable development of agriculture.

Method used

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  • A kind of growth-promoting composite microbial bacterial agent and its application
  • A kind of growth-promoting composite microbial bacterial agent and its application
  • A kind of growth-promoting composite microbial bacterial agent and its application

Examples

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preparation example Construction

[0035] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: after activating the strain, carry out continuous two-stage seed liquid culture, insert into the fermentation medium for fermentation, and concentrate to obtain concentrated bacterial liquid; The concentrated bacterial liquid of Kashmir small strange bacteria BQB-B04 is mixed with a protective agent (skimmed milk powder) and then freeze-dried to obtain the solid bacterial powder; the fermentation of the Bacillus Velez YZS-M03 and the Bacillus highlander ZLB-C01 The solid bacterial powder is obtained by mixing the solution with light calcium carbonate and spray-drying.

[0036] In the present invention, the strains are inoculated in NB medium and cultured for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is preferably 150-350 r min -1 .

[0037] In the present invention, after the pr...

Embodiment 1

[0041] Example 1 Preparation of growth-promoting compound microbial bacterial agent

[0042] (1) Preparation of BQB-B04 bacteria liquid and solid bacteria powder of Kashmir small strange bacteria:

[0043] Preparation of first-grade seed solution of BQB-B04 small strange bacteria in Kashmir

[0044] Activate the BQB-B04 strain of Kashmiri small strange bacteria, pick a single colony, and inoculate it in 150ml NB medium (sterilize for 30min at 121°C), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0045] Preparation of secondary seed solution of BQB-B04 small strange bacteria in Kashmir

[0046] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 25℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 10~...

Embodiment 2

[0066] Example 2 The effect of growth-promoting compound microbial bacterial agent liquid preparation 1 on rape leaves

[0067] With the growth-promoting composite microbial bacterial preparation liquid preparation 1 prepared in Example 1 and the single-species fermented mature bacterial liquid of Bacillus Bacillus YZS-M03, Kashmir small strange bacterium BQB-B04 and Bacillus highlander ZLB-C01, use respectively Sterile water is configured so that the live bacteria content is 1×10 8 The cfu / mL bacterial suspension was used to inoculate rapeseed seedlings by root irrigation (inoculation volume: 20mL / plant), 10 plants per treatment, repeated 3 times, and clear water was used as the control. Chlorophyll content was measured 15 days after the inoculation treatment, and 10 pieces of plant leaves were collected and mixed thoroughly as a mixed sample for catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) ) and other indicators.

[0068] Using a SPAD-502 chlorophyll rap...

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Abstract

The invention relates to a growth-promoting compound microbial bacterial agent and an application thereof. The bacterial agent includes Bacillus Velez YZS-M03, Bacillus Kashmir BQB-B04 and Bacillus alboland ZLB-C01. The constituent strains of the growth-promoting composite microbial bacterial agent have strong phosphorus-dissolving, potassium-dissolving and nitrogen-fixing effects, and can colonize in plants. The growth-promoting compound microbial bacterial agent can increase plant chlorophyll content, increase plant biological enzyme activity, and promote plant growth and development.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a growth-promoting composite microbial bacterial agent and an application thereof. Background technique [0002] Problems such as poor permeability, poor water and fertilizer retention, and poor soil fertility in the current soil seriously affect the sustainable development of agriculture. Therefore, improving soil, improving soil fertility, improving soil ecological environment, and improving crop yield and quality have become the focus of current agricultural work. Microorganisms play a very important role in the input and output of materials and energy in soil, and are an important link in the material cycle chain. It can activate soil organic and inorganic nutrients, decompose organic matter, release nutrients, and increase the availability of nutrients. In the process of reproduction and metabolism, microorganisms can degrade the residual chemical fertilizers, organic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00A01G7/06A01G22/05A01G22/15C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/20A01G7/06A01G22/05A01G22/15
Inventor 袁宗胜刘芳肖航曾志浩陈新强林鸿艳陈垚宇谢宝贵
Owner MINJIANG UNIV
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