Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Growth-promoting compound microbial agent and application thereof

A technology of compound microbial agent and microbial strain, which is applied in the field of microorganisms, can solve the problems that affect the sustainable development of agriculture, poor water and fertilizer retention, and poor soil fertility.

Active Publication Date: 2021-04-30
MINJIANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Problems such as poor permeability, poor water and fertilizer retention, and poor soil fertility in the current soil seriously affect the sustainable development of agriculture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Growth-promoting compound microbial agent and application thereof
  • Growth-promoting compound microbial agent and application thereof
  • Growth-promoting compound microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: after activating the strain, carry out continuous two-stage seed liquid culture, insert into the fermentation medium for fermentation, and concentrate to obtain concentrated bacterial liquid; The concentrated bacterial liquid of Kashmir small strange bacteria BQB-B04 is mixed with a protective agent (skimmed milk powder) and then freeze-dried to obtain the solid bacterial powder; the fermentation of the Bacillus Velez YZS-M03 and the Bacillus highlander ZLB-C01 The solid bacterial powder is obtained by mixing the solution with light calcium carbonate and spray-drying.

[0036] In the present invention, the strains are inoculated in NB medium and cultured for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is preferably 150-350 r min -1 .

[0037] In the present invention, after the pr...

Embodiment 1

[0041] Example 1 Preparation of growth-promoting compound microbial bacterial agent

[0042] (1) Preparation of BQB-B04 bacteria liquid and solid bacteria powder of Kashmir small strange bacteria:

[0043] Preparation of first-grade seed solution of BQB-B04 small strange bacteria in Kashmir

[0044] Activate the BQB-B04 strain of Kashmiri small strange bacteria, pick a single colony, and inoculate it in 150ml NB medium (sterilize for 30min at 121°C), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0045] Preparation of secondary seed solution of BQB-B04 small strange bacteria in Kashmir

[0046] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 25℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 10~...

Embodiment 2

[0066] Example 2 The effect of growth-promoting compound microbial bacterial agent liquid preparation 1 on rape leaves

[0067] With the growth-promoting composite microbial bacterial preparation liquid preparation 1 prepared in Example 1 and the single-species fermented mature bacterial liquid of Bacillus Bacillus YZS-M03, Kashmir small strange bacterium BQB-B04 and Bacillus highlander ZLB-C01, use respectively Sterile water is configured so that the live bacteria content is 1×10 8 The cfu / mL bacterial suspension was used to inoculate rapeseed seedlings by root irrigation (inoculation volume: 20mL / plant), 10 plants per treatment, repeated 3 times, and clear water was used as the control. Chlorophyll content was measured 15 days after the inoculation treatment, and 10 pieces of plant leaves were collected and mixed thoroughly as a mixed sample for catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) ) and other indicators.

[0068] Using a SPAD-502 chlorophyll rap...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a growth-promoting compound microbial agent and application thereof. The microbial agent is prepared from bacillus velezensis YZS-M03, Advenella kashmirensis BQB-04 and bacillus altitudinis ZLB-C01. According to the invention, the composition strain of the growth-promoting compound microbial agent has relatively strong phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing effects and can be colonized in a plant body; and the growth-promoting compound microbial agent can improve the chlorophyll content of plants, improve the biological enzyme activity of the plants and promote the growth and development of the plants.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a growth-promoting composite microbial bacterial agent and an application thereof. Background technique [0002] Problems such as poor permeability, poor water and fertilizer retention, and poor soil fertility in the current soil seriously affect the sustainable development of agriculture. Therefore, improving soil, improving soil fertility, improving soil ecological environment, and improving crop yield and quality have become the focus of current agricultural work. Microorganisms play a very important role in the input and output of materials and energy in soil, and are an important link in the material cycle chain. It can activate soil organic and inorganic nutrients, decompose organic matter, release nutrients, and increase the availability of nutrients. In the process of reproduction and metabolism, microorganisms can degrade the residual chemical fertilizers, organic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00A01G7/06A01G22/05A01G22/15C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/20A01G7/06A01G22/05A01G22/15
Inventor 袁宗胜刘芳肖航曾志浩陈新强林鸿艳陈垚宇谢宝贵
Owner MINJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com