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Method of raising taxol compound content in cultured taxad

A technology of taxanes and yews, which is applied in the field of plants, can solve the problems of low content of taxanes, achieve the effects of low content, high economic value, and broad application prospects

Inactive Publication Date: 2007-10-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to solve the problem of low taxane compound content in the above-mentioned yew, and provide a method that can increase the taxane compound content in the cultivated yew

Method used

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  • Method of raising taxol compound content in cultured taxad
  • Method of raising taxol compound content in cultured taxad
  • Method of raising taxol compound content in cultured taxad

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Soil cultivation

[0020] 1. Plant Material and Handling

[0021] The materials for field cultivation experiments were biennial Taxus x media purchased from Chengdu Plant Micropropagation Co., Ltd. in May 2003 and planted in the Taxus Experimental Garden, Shangcao Town, Lianshan County, Qingyuan City, Guangdong Province Medium (647m above sea level). Every plant was weighed for the first time when it was cultivated on May 26, 2003, and was dug out with roots on May 26, 2004. After washing the soil substrate with water, it was weighed again. The soil for planting is loose and fertile, suitable for the growth of yew.

[0022] The experiment set up 14 groups of Taxus×media small trees, 20 trees in each group, 12 groups were used for the experiment, and 2 groups were used as controls. Each experimental group was applied with different doses of blue vitriol solution (see Table 1). Branches and leaves of each plant in each group were collected on March 26, 2004,...

Embodiment 2

[0030] Example 2 Hydroponics

[0031] 1. Plant Material and Handling

[0032] Annual Taxus wallichiana var. mairei saplings used for hydroponic experiments were purchased on June 24, 2003 at the Dadongshan Nature Reserve in northern Guangdong Province, China. All seedlings used in experiments were screened for similar height and approximate biomass. The soil matrix in the root system was rinsed with double distilled water and handled with as little root damage as possible. Then the plants are transferred to 32 liters (17 (high) * 65 (long) * 29 (wide) centimeters) in the plastic container that is filled with corresponding treatment nutrient solution, in the medium, use air pump to pass through continuously, evenly with gas, Ten holes with a diameter of 6 cm were perforated on the plastic plate of the medium plastic container. Each plant was fixed in a long hole with a rubber stopper with a small hole split in the middle, and every two hydroponic Taxus wallichiana var. maire...

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Abstract

The present invention discloses one method of raising taxane compound content in cultured taxad. Under proper taxad cultivating conditions, copper containing compound is applied as the fertilizer additive, and the applied amount of the copper containing compound is 0.00002-0.0002 mole each plant of soil cultivated taxad or Cu ion concentration of 0.0001-0.001 mol / L in water solution for cultivating taxad. The method can raise the taxane compound content in cultured taxad, and the taxane compound has very high economic value.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for increasing the content and yield of taxane compounds in the cultivated yew. Background technique [0002] Taxus spp., commonly known as "water pine", is a national first-class protected plant, containing taxane compounds, and its secondary metabolite paclitaxel is a specific drug for the treatment of breast cancer and ovarian cancer, and it is effective for lung cancer, Kaposi's sarcoma It is also a broad-spectrum and highly active anticancer drug recognized in the world today. It can specifically promote the polymerization of tubulin and block cell division. It is a powerful tool for cytological research. The content of paclitaxel in the slow-growing yew is very low, the cost of extraction and separation is very high, and the price is very expensive. The increasing demand for clinical and scientific research has led to the disappearance of yew in many places, resulting in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H3/04
Inventor 王昌伟彭少麟李鸣光李晓龙
Owner SUN YAT SEN UNIV