Formulation, which can be administered gastrointestinally, and uses thereof
A gastrointestinal and preparation technology, applied in the direction of drug combinations, surgical drugs, medical preparations containing active ingredients, etc., can solve the life-threatening problems of surgical patients
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[0083] The following examples are intended to illustrate the invention without limiting it.
[0084] ischemia-reperfusion model
[0085] Every surgical procedure involves reversible ischemia of organs and tissues caused by the temporary clamping of blood vessels. This ischemic condition can be mimicked in a "low-flow, high-flow" hepatic perfusion model in rats.
[0086] In this rat model, the liver is perfused at a low flow rate to produce a localized oxygen deficiency, ie, hypoxia, in the pericentral hepatic zone through reduced oxygen supply. Subsequent reperfusion of the liver at normal flow rates brings oxygen back to previously hypoxic hepatic regions, which then leads to the formation of oxygen-dependent free radicals and consequent reperfusion injury. Much of the resulting cell death in the pericentral hepatic zone releases cell-bound enzymes, such as lactate hydrogenase and transaminases, which can be detected and quantified in the perfusate.
[0087] Sprague-Dawley...
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