Recombinant plasmid with human thymosin Beta-4gene

Abstract

A recombinant plasmid with human extrasin beta4-gene carrier is prepared by inserting human extrasin beta4-gene into eukaryote expression starter to construct recombinant plasmid, entering it into striped muscle cell, synthesizing T beta-4 by cell protein synthetic system and secreting to extracellular region. It can improve wound healed, prevent cell from death and be used to repair skin and heart damages.

Description

1. Technical field [0001] The invention belongs to the field of gene therapy, specifically, it is a recombinant plasmid constructed by inserting human thymosin β4 gene into a eukaryotic expression promoter. The plasmid can enter striated muscle cells, and use the protein synthesis system of the cell itself to synthesize Tβ4 in the cell, and secrete it out of the cell, so as to accelerate wound healing and prevent cell death. 2. Background technology [0002] The immune system is the body's defense system. The main cells involved in the immune response are T lymphocytes, B lymphocytes and macrophages. The thymus is the central immune organ for the development and differentiation of T lymphocytes. Thymus factors (or hormones) secreted by the thymus are a series of essential substances required for the development and differentiation of T lymphocytes, among which thymosin β4 (Tβ4) is a major thymus factor whose structure and function have been clarified. Thymosin-beta 4 enha...

AI Technical Summary

Problems solved by technology

However, at present, the vectors used to carry Tβ4 as gene therapy mainly include adenovirus vector (AV) and adeno-associated virus vector (AAV). The infection rate is very high, and it is easy to carry genes into human cells. However, it is precisely because of their viral characteristics that the safety of gene therapy has become a concern.

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