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Bacillus of high proteinase yield and its induction mutation breeding method and uses

A bacillus and protease technology, applied in the direction of bacteria, application, and microbial-based methods, can solve the problems of high cost of active peptide feed additives and difficult promotion and use, and achieve the effects of low price, reduced fermentation cost, and convenient source

Inactive Publication Date: 2008-09-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the high cost of preparing active peptide feed additives in the prior art and is not easy to popularize and use in the animal breeding industry, and to breed a Bacillus strain capable of high protease production through physical and chemical compound mutagenesis to make it It can be produced by fermentation with low addition amount in feed, clear function, and can improve the absorption and utilization of nutrients (such as protein, minerals, vitamins, etc.) by animals, increase the output and production capacity of milk, meat, eggs, and improve milk , the quality and flavor of meat and eggs, stimulate the development of animal immune organs, improve disease resistance and reduce morbidity, and can significantly improve the water quality of aquatic animal breeding, increase the growth rate and survival rate of aquatic animals, and can be widely used in poultry, cattle, Active peptide feed additives that can be used as a substitute for antibiotics for sheep, pigs, aquatic products and economic animals

Method used

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  • Bacillus of high proteinase yield and its induction mutation breeding method and uses
  • Bacillus of high proteinase yield and its induction mutation breeding method and uses
  • Bacillus of high proteinase yield and its induction mutation breeding method and uses

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Embodiment 1

[0038] Embodiment 1, mutagenesis breeding the bacillus that can produce high protease of the present invention

[0039] According to the mutagenesis breeding method provided by the present invention, mutagenesis and breeding can produce protease-rich bacillus, and its steps are as follows:

[0040] 1. The Bacillus subtilis CAU208 purchased from the "General Microbiology Center of China Microbiological Culture Collection Management Committee" with the preservation number CGMCCNo1.769 is used as the starting strain;

[0041] 2. Mutation breeding:

[0042] (1) Preparation of single cell suspension of starting strain CAU208

[0043] The starting strain Bacillus CAU208 was inoculated in the sterilized liquid medium A, cultured in a shake flask at 30°C for 36 hours, centrifuged with 1ml of the seed solution, washed 3 times with sterile saline, and placed in a sterile glass bead container. In the triangular flask, vibrate for 20 minutes to disperse into a single-cell suspension, fi...

Embodiment 2

[0058] Embodiment 2, mutagenesis breeding the bacillus that can produce high protease of the present invention

[0059] According to the mutagenesis breeding method provided by the present invention, mutagenesis and breeding can produce protease-rich bacillus, and its steps are as follows:

[0060] 1. The Bacillus subtilis CAU208 purchased from the "General Microbiology Center of China Microbiological Culture Collection Management Committee" with the preservation number CGMCCNo1.769 is used as the starting strain;

[0061] 2. Mutation breeding:

[0062] (1) Preparation of single cell suspension of starting strain CAU208

[0063] The starting strain Bacillus CAU208 was inoculated in the sterilized liquid medium A, cultured in a shaker flask at 28°C for 48 hours, centrifuged with 1ml seed solution, washed 3 times with sterile saline, and placed in a sterile glass bead container. In the triangular flask, vibrate for 20 minutes to disperse into a single-cell suspension, filter i...

Embodiment 3

[0078] Embodiment 3, mutagenesis breeding the bacillus that can produce high protease of the present invention

[0079] According to the mutagenesis breeding method provided by the present invention, mutagenesis and breeding can produce protease-rich bacillus, and its steps are as follows:

[0080] 1. The Bacillus subtilis CAU208 purchased from the "General Microbiology Center of China Microbiological Culture Collection Management Committee" with the preservation number CGMCCNo1.769 is used as the starting strain;

[0081] 2. Mutation breeding:

[0082](1) Preparation of single cell suspension of starting strain CAU208

[0083] The starting strain Bacillus CAU208 was inoculated in the sterilized liquid medium A, cultured in a shake flask at 30°C for 42 hours, centrifuged with 1ml seed solution, washed 3 times with sterile saline, and placed in a sterile glass bead container. In the triangular flask, vibrate for 20 minutes to disperse into a single-cell suspension, filter it ...

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Abstract

The invention relates to bacillus for high-yield protease with systematic name as Bacillus sp, which is preserved in general microbe center of CGMCC with number as CGMCC No.1180 in 6,28,2004. Wherein, by CAU208 with number CGMCC No1.769 as initial strain, recycling the process of variable ultraviolet mutagenesis->NTG mutagenesis->composite mutagenesis to CAU208 monocell; finally, obtaining the product. This invention can be used in preparing soy biological active peptide feed additive and other products. Wherein, the said peptide additive has a plurality of applications: increasing egg quality, satisfying nutrition require of animal with immature digestive system, etc.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus capable of high protease production and its mutagenesis selection method and application. Background technique [0002] For many years, antibiotics, chemical synthetic drugs, hormones and other growth-promoting health care agents have been widely used in animal feeding and feed, resulting in serious drug residues in livestock and aquatic products, decline in the quality of livestock and poultry products, increased bacterial resistance, and drug-resistant strains The increase poses a great threat to human security and the ecological environment. Due to the many side effects of antibiotics, especially consumers' attention to food safety in recent years, countries around the world are banning or restricting the use of antibiotics, and accelerating the development and promotion of new safe and efficient green feed additives to reduce or replace antibiotics, etc....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07A23K1/165A23K1/16A23K10/12A23K20/147A23K20/189
Inventor 张日俊于长青
Owner CHINA AGRI UNIV
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