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Self-illuminating biological chip and producing and detecting method thereof

A biochip and manufacturing method technology, which is applied in biological testing, material analysis by optical means, material inspection products, etc., to achieve the effects of easy industrialization and promotion, easy product quality, and easy operation.

Inactive Publication Date: 2008-10-22
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the application of its fluorescence principle and the principle of light intensity change when the measured object interacts with the active substance of the specific recognition function on the chip to make a biochip, no bibliographical report identical to the present invention has been found after searching.

Method used

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  • Self-illuminating biological chip and producing and detecting method thereof
  • Self-illuminating biological chip and producing and detecting method thereof

Examples

Experimental program
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Embodiment 1

[0025] A self-luminous biological chip designed by the present invention uses a silicon wafer (1) as a carrier, and prepares a porous silicon chip capable of emitting fluorescence and having uniform nanopores by means of electrochemical corrosion. The preparation conditions and methods are as follows: the corrosion solution used : 20% hydrofluoric acid is poured into a polytetrafluoroethylene container; electrodes used: platinum electrodes are cathodes, silicon wafer electrodes are anodes, and an adjustable DC stabilized current power supply is used, with a current density of 50mA / cm 2 , energized for 15 minutes, the porous silicon wafer (1) that can be made evenly has nanopore (2) and its diameter is 10 nanometers, as figure 2 As shown in (a), emitted light (3) can be seen. Immobilizing active substances on porous silicon chips: Prepare a lectin solution with a concentration of 10 μg / ml with 50 mM pH6.8 carbonate buffer or other flushing solutions, and put 0.5 ml of the prep...

Embodiment 2

[0027] Corrosive solution used: 15% hydrofluoric acid; current density 150mA / cm 2 , energized for 10 minutes, the porous silicon wafer (1) can be uniformly opened with nanopores (2) whose diameter is 50 nanometers. Immobilize the active substance on the porous silicon chip: prepare a β-lactamase solution with a concentration of 40 μg / ml with 80 mM pH7.0 phosphate buffer or other washing solution; take 1 ml of the prepared β-lactamase solution and put it into a test tube; The porous silicon chip is immersed in the solution in the test tube, and placed in a refrigerator for fixation reaction. The reaction time is 11 hours; then the porous silicon chip (1) is taken out, and rinsed 5 times with 50mM pH7.0 phosphate buffer saline, that is A biochip attached with β-lactamase can be made. All the other are identical with embodiment 1.

Embodiment 3

[0029] Corrosive solution used: 25% hydrofluoric acid; current density 40mA / cm 2 After energizing for 20 minutes, the porous silicon wafer (1) can be uniformly opened with nanopores (2) whose diameter is about 30 nanometers. Immobilize the active substance on the porous silicon chip: prepare a CK-MB antibody solution with a concentration of 50 μg / ml with 100mM pH7.8 phosphate buffer or other flushing solutions, take 2ml of the prepared CK-MB antibody solution into a test tube, and place the porous The silicon chip is immersed in the test tube; the fixed reaction time is 16 hours in the refrigerator. Then, the porous silicon chip (1) is taken out, rinsed 6 times with 50mM pH7.0 phosphate buffer or other buffer, and then the biochip attached with CK-MB antibody can be made. All the other are identical with embodiment 1

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Abstract

The present invention provides a self-luminescent biological chip and its production and detection method. It is characterized by that on the carrier silicon wafer a nano hole with 10-50 nanometers is cut, it can naturally form emission light, on the nano hole an active substance with specific identification function is fixed. Said nano hole also has emission light, when it is contacted with tested material, its light intensity can be changed, so that the concentration of the tested material can be detected. The described active substance is CK-MB antibody, beta-lactamase or lectin.

Description

technical field [0001] The invention relates to a biochip and its manufacturing method, specifically a biochip for detecting sample concentration and its manufacturing method. The international patent classification number is intended to be Int.Cl 7 G01N33 / 50. Background technique [0002] Existing biochips or biosensors and their production and detection methods generally require enzymes, colors, light or other labeling substances as media. For example, the existing blood glucose biochips or sensors are made by immobilizing glucose enzymes on a carrier (such as glass, film, PVC, etc.), and adding more than one substrate and related substances as media. After the blood glucose in the detection sample contacts and combines with the glucose enzyme on the chip, a series of reactions occur with the substrate, etc., and a specific light or electrical signal is generated, thereby being detected. For another example, immunological biochips or sensors are usually: first, antibodie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17G01N33/50G01N33/531
Inventor 张娟琨贾士儒许增朴于德敏林永贤苏建宇宋诗莹
Owner TIANJIN UNIV OF SCI & TECH