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Lindera root alkaloid, its preparation method and application in medicine preparation

A kind of technology of alkaloid and black medicine, which is applied in the direction of drug combination, medical formula, antipyretic, etc., can solve the problems of damage to gastrointestinal mucosa, poor action selectivity, induction of infection, etc., to alleviate rheumatic diseases, reduce content, The effect of reducing swelling

Active Publication Date: 2009-01-21
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For rheumatoid arthritis, non-steroidal anti-inflammatory drugs (diclofenac, aspirin, etc.) and immunosuppressants (6-mercaptopurine, etc.) are currently mainly used for treatment, but these drugs all have obvious adverse reactions, such as the former damages the gastrointestinal tract tract mucosa leading to peptic ulcer, which has poor selectivity and is easy to induce infection, etc.

Method used

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  • Lindera root alkaloid, its preparation method and application in medicine preparation
  • Lindera root alkaloid, its preparation method and application in medicine preparation
  • Lindera root alkaloid, its preparation method and application in medicine preparation

Examples

Experimental program
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Effect test

example 1

[0031] The preparation of example 1, black medicine alkaloid

[0032] Method: Weigh 2 kg of black herb powder, heat and reflux with 90% ethanol to extract 3 times, the amount of 90% ethanol is 12000mL each time, extract for 2 hours each time, combine the extracts, filter, concentrate to dryness under reduced pressure, add 2 2000mL of % HCl solution, dissolved in alkaloids, filtered, and the filtrate was adjusted to pH 8.5 with ammonia water, stirred thoroughly, and left to stand for 5 hours. After the precipitate was fully analyzed, the precipitate was filtered out, and dried under reduced pressure at 55°C to obtain the black medicine Alkali is a light brown powder. The weighed weight is 32g. The yield is 1.60%. After determination, the content of total alkaloids is 53.13%, and the content of norisoboldine is 33.84%.

example 2

[0033] Example 2, the influence of alkaloids of the present invention on mouse arthritis caused by type II collagen

[0034] Chicken type II collagen and Freund's complete adjuvant were fully ground and emulsified in a cold water bath to prepare 1.5mg / ml. Male ICR mice were intradermally injected with 100 μl of the above-mentioned emulsion at the base of the tail. The same operation was taken on the 21st day, and the mice were grouped by body weight. The administration group was fed with 50, 100, and 200 mg / kg of alkaloids of the present invention, and the positive control group was fed with dexamethasone (dexamethasone acetate sheet, Zhejiang Xianju Pharmaceutical Co., Ltd. product, batch number 040902) 1mg / kg and tripterygium glycosides (tripterygium glycosides tablets, product of Shanghai Fudan Fuhua Pharmaceutical Co., Ltd., batch number 020601) 15mg / kg. The control group and the normal group were intragastrically administered equal volume of distilled water, once a day, ...

example 3

[0049] Example 3, the effect of in vitro administration of alkaloids of the present invention on the release of nitric oxide from LPS-induced mouse peritoneal macrophages

[0050] Male ICR mice were sacrificed by decapitation and bloodletting, and peritoneal macrophages were collected under sterile conditions, and the cell concentration was adjusted to 2×10 6 pieces / ml. Add the cells to a 96-well plate, the number of cells per well is 2×10 5 , adhere to the wall for 2 hours, wash twice with RPMI-1640. Add LPS (final concentration of 10 μg / ml) and different concentrations of drugs (dissolved in RPMI-1640 and DMSO, final concentration of DMSO is 0.5%) to each well, incubate for 24 hours, absorb 100 μl of supernatant, and react with the same amount of Griess reagent for 10 Minutes, the OD value was measured at 540nm.

[0051] It can be seen from Table 5 that 50 and 100 μg / ml of alkaloids of the present invention significantly inhibited the release of NO from mouse peritoneal m...

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Abstract

The invention discloses the use of spicebush root alkaloid in preparing medicament for treating chronic infectious arthritis and other immune diseases. Animal tests show that, the spicebush root alkaloid can substantially alleviate the degree of mouse joint pathological changes, and lower the mouse feet tumefaction degree caused by arthralgia due to wind-cold-dampness.

Description

Technical field: [0001] The invention relates to the technical field of traditional Chinese medicine. In particular, it relates to the alkaloid of black medicine, its preparation method and its application in pharmacy. Background technique [0002] Rheumatoid arthritis is a common clinical disease and frequently-occurring disease, which belongs to autoimmune disease. For rheumatoid arthritis, non-steroidal anti-inflammatory drugs (diclofenac, aspirin, etc.) and immunosuppressants (6-mercaptopurine, etc.) are currently mainly used for treatment, but these drugs all have obvious adverse reactions, such as the former damages the gastrointestinal tract Tract mucosa leads to peptic ulcer, which has poor selectivity and is easy to induce infection. Therefore, some effective parts of traditional Chinese medicine with clear active ingredients, controllable quality, safety and high efficiency have potential value in the development of rheumatoid arthritis therapeutic drugs. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/473A61K31/472A61P29/00A61P19/02A61P37/02
Inventor 侴桂新戴岳王峥涛周吉燕
Owner SHANGHAI UNIV OF T C M
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