Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel phenolic acid compounds of tanshin polyphenolic acid N and application thereof

A kind of technology of salvianolic acid and compound, which is applied to phenolic acid compound salvianolic acid N and its application field, and can solve problems such as unreported

Inactive Publication Date: 2009-02-04
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salvianolic acid N of the present invention is just a new compound in Salvia miltiorrhiza discovered in a large number of screening research processes, and the compounds related to this structure have not been reported so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel phenolic acid compounds of tanshin polyphenolic acid N and application thereof
  • Novel phenolic acid compounds of tanshin polyphenolic acid N and application thereof
  • Novel phenolic acid compounds of tanshin polyphenolic acid N and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 .method of extraction

[0023] Take 4 kg of the root of Danshen medicinal material, cut into decoction pieces, add 8 times the amount of water to soak for 0.5 hour, decoct and extract for 1 hour, filter, and the filtrate is set aside. The dregs were decocted with 6 times the amount of water to extract twice, each time for 0.5 hours, filtered, the filtrates were combined three times, concentrated under reduced pressure, and 0.8 kg of extract was obtained.

Embodiment 2

[0024] Example 2 .Purification method

[0025] Add 5 times the amount of water to the extract extract and heat to dissolve, slowly inject into the processed HP20 type, SP825 type macroporous adsorption resin column, first elute with water, discard; then elute with 50% ethanol, collect medicinal materials 3 Double the amount of 50% ethanol eluate, concentrate, and dry to obtain 170 g of total phenolic acids.

[0026] Heat and dissolve the total phenolic acids with water, slowly add to the Sephadex LH-20 column, first elute with water, then elute with 2 times column volume of 40% ethanol, discard; then use 60% of 2 times column volume Eluted with ethanol, the eluate was collected, concentrated, and purified by repeated column chromatography on an ODS column to obtain the new compound salvianolic acid N.

Embodiment 3

[0027] Example 3 .Structure Identification:

[0028] Salvianolic acid N: yellow amorphous powder, C 26 h 20 o 10 , with 1% FeCl 3 Reagent reaction is brown, ESI-MS ( figure 1 ): 515 (M+Na) + 、493(M+H) + 、295(M-197-Na) + . 1 HNMR (CD 3 OD)δ( image 3 ): 7.88 (1H, d, J=16.0Hz, H-7) and 6.22 (1H, d, J=16.0Hz, H-8) are a group of trans double bond proton signals, the gHMBC ( Figure 6 ) spectrum hints that this group of trans double bond protons is conjugated with carbonyl, 7.27 (1H, d, J=8.5Hz, H-7″) and 6.81 (1H, d, J=8.5Hz, H-8″) are A set of cis double bond proton signals, 3.05(1H, dd, J=15.0 / 4.0Hz, H α -7'), 2.96 (1H, dd, J=15.0 / 8.0Hz, H β -7') and 5.14 (1H, dd, J=8.0 / 4.0Hz, H-8'), suggesting that there is -CH(O)-CH in the compound 2 -Structural fragments, a set of ABX coupling systems that bind aromatic regions, 6.70 (1H, d, J = 1.5Hz, H-2'), 6.66 (1H, d, J = 8.0Hz, H-5') and 6.57 ( 1H, dd, J=1.5 / 8.0Hz, H-6'), indicating that there is a β-(3,4-dihydroxypheny...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to one kind of phenolic acid compound and its preparation process and use in treating AIDS. Red sage material is treated through solvent extraction, macroporous adsorbing resin purification, conventional drying and other steps to obtain total phenolic acid. The total phenolic acid is dissolved in water via heating, chromatographic column separation and repeated purification to obtain new compound salvianolic acid N. Enzyme and cell activity experiments show that the new compound has HIV resisting activity and is hopeful in being developed into one new kind of AIDS treating medicine.

Description

Technical field: [0001] The invention relates to a new phenolic acid compound in salvia miltiorrhiza and its application in anti-AIDS. Background technique: [0002] Salvia miltiorrhiza is the root of Salvia genus Lamiaceae. It is bitter in nature and slightly cold. Modern pharmacological studies have proved that Danshen can expand coronary arteries, increase coronary flow, improve microcirculation, and protect the heart. It can inhibit and relieve platelet aggregation, improve the body's ability to withstand hypoxia, and have anti-hepatitis, anti-tumor, and anti-virus activities. In 1988, the Institute of Medical Biotechnology of the Chinese Academy of Medical Sciences found that the water extract of Salvia Miltiorrhiza had significant anti-HIV and anti-HBV effects through the research of antiviral traditional Chinese medicines, and discussed the "Use of Salvia Miltiorrhiza for Anti-AIDS Virus and Anti-Hepatitis B Virus" Applied for a Chinese invention patent (patent numbe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D313/14A61K31/335A61P31/18
Inventor 陈鸿珊张正付彭宗根蒋建东
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com