Method for making micro-fluidic chip with Z-shape spectrophotometric detection cell

Abstract

The related preparation method for micro flow control chip with Z-shape luminosity detector comprises: 1) drawing figure for top and lower layers by a graphic software for output; 2) light etching to prepare two male moulds for alkylation; 3) adding a U-shape washer between former moulds to form a U-shape liquid slot; 4) pouring dimethyl silicone polymer and initiator processed in vacuum, heating for curing; 5) unfolding moulds to obtain the PDMS film; 6) preparing top and lower covers; 7) sticking cover to the PDMS film, irradiating with light source, and obtaining the final product. This invention is simple and low cost, and fit to wide application.

Description

technical field [0001] The invention relates to a method for manufacturing a chip, in particular to a method for manufacturing a microfluidic chip with a Z-type photometric detection flow cell. Background technique [0002] Microfluidic system is an analytical technology developed in the 1990s. It utilizes microfabrication technology to fabricate functional units such as microchannels, microreactors, and microdetectors on chips to form a microsystem, which is used to complete traditional chemistry and The operation process of biological analysis and detection has the characteristics of portability, high throughput, flexible functions, low sample and low reagent consumption, etc. [0003] Absorbance detection is an important detection method, which is widely used in liquid phase systems and capillary electrophoresis systems. However, there are few studies on absorbance detection directly on microfluidic chips. The main reasons are: (1) microfluidic The channel diameter of th...

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Problems solved by technology

However, it is difficult to integrate the Z-type photometric detection flow cell into the microfluidic chip: (1) It is not easy to make a zigzag channel on the microfluidic chip and detect it in a direction parallel to the chip , because the side walls of the microchannels made by the current low-cost method are mostly arc-shaped, and the optical path needs to introduce components such as optical fibers, which are not easy to process and the measurement repeatability is not good; The absorption cell of the above-mentioned absorption cell, its cost is too high; And the method that the channel is etched on the upper and lower coverslips is not suitable for making Z-type flow cell, because the light window on both sides made by common etching method is not easy to make plane; (3) The method of using an external detection cell for microfluidic chip photometric detection is not an ideal solution because the increase in the dead volume will cause signal broadening, and at the same time, the cost will be too high due to the complexity of the system.

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