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Method for regulating and controlling nerve stem cell differentiation, and restraining differentiation

A neural stem cell, silencing technology, applied in the field of inhibiting the differentiation of neural stem cells, can solve problems such as lack of cure methods

Inactive Publication Date: 2009-07-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, the treatment of these central nervous system diseases, whether it is drug treatment or surgical treatment, is only a symptomatic treatment, and there is no effective cure.

Method used

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  • Method for regulating and controlling nerve stem cell differentiation, and restraining differentiation
  • Method for regulating and controlling nerve stem cell differentiation, and restraining differentiation
  • Method for regulating and controlling nerve stem cell differentiation, and restraining differentiation

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Experimental program
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Embodiment Construction

[0038] This embodiment includes the following steps:

[0039] 1. Construction of recombinant fluorescent plasmid vector pEGFP-C1-DCF1

[0040] pEGFP-C1 is a mammalian expression vector, the green fluorescent protein GFP encoded by the reporter gene can emit green fluorescence at 510nm when excited by near-ultraviolet light at 395nm or blue light at 470-490nm. The upstream and downstream primers were designed according to the full-length gene sequence of DCF1, and the full-length gene DCF1 was amplified by PCR using the neural stem cell mRNA library as a template. The DCF1 gene was inserted into the multiple cloning site of the fluorescent plasmid vector pEGFP-C1 to construct the recombinant vector pEGFP-C1-DCF1.

[0041] The sequences of the upstream and downstream primers are as follows. In order to facilitate the connection with the vector, the upstream primer of the Kpn1 adapter site is put in: 5'-CTAT GGTACC ATGGCGGCGCCAAAGGGGAA-3'

[0042] Kpn1

[00...

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Abstract

The invention provides a technique for regulating gene expression related to the differentiation and development of neural stem cells. The present invention also provides the corresponding expression vector and nucleic acid sequence of the technology. The present invention provides detailed steps for implementing this technique.

Description

technical field [0001] The present invention relates to a regulation method for neural stem cell differentiation and differentiation suppression, in particular, dendritic cellfactor (DCF1) gene is used to inhibit neural stem cell differentiation; and the neural stem cell differentiation is induced by silencing DCF1. Background technique [0002] The aging of the world's population has led to an increase in the incidence of neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, which has become a growing social problem. At the same time, various nervous system injuries, such as spinal cord injury and ischemic stroke, also seriously threaten human health. For a long time, the treatment of these central nervous system diseases, whether it is drug treatment or surgical treatment, is only a symptomatic treatment and lacks an effective cure. In recent years, the progress of neural stem cell research has opened up a new path for the treatment of nervous sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C12N15/12C12N15/85C12N5/10
Inventor 文铁桥王蕾庞淑亚
Owner SHANGHAI UNIV