Reagent formula for fastly inspecting glutamic-pyruvic transaminase by optical method

An alanine aminotransferase and reagent technology, which is applied in biological testing, material analysis and material inspection by observing the impact on chemical indicators, etc., can solve the problems of reagent interference factors, large errors, and difficulty in promotion and popularization.

Active Publication Date: 2009-10-07
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

However, the ketone body powder method has no strict measurement conditions, and there are many interference factors in the reagents. The reaction to determine the result is a qualitative reaction, with a high false positive rate and large errors, and has been explicitly eliminated by the Ministry of Health; (ALT) can only be operated manually, which is labor-intensive and time-consuming (one measurement takes 30 minutes), and the detection reagent is easily affected by factors such as fat in serum, hemolysis, jaundice, etc., so it is difficult to popularize

Method used

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  • Reagent formula for fastly inspecting glutamic-pyruvic transaminase by optical method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A reagent was prepared from the concentrations indicated for the following compounds:

[0037] Compound Concentration

[0038] L-alanine 90.0mM / L

[0039] α-ketoglutarate 4.0mM / L

[0040] Sodium Phosphate 30mM / L

[0041] Pyruvate oxidase 300.0U / mL

[0042] Peroxidase 600.0U / mL

[0043] 4-Aminoantipyridine 40.0mM / L

[0044] Thiamine pyrophosphate 0.11mM / L

[0045]Flavin adenine dinucleotide 0.30mM / L

[0046] 5'-Pyridoxal phosphate 0.50mM / L

[0047] Sodium alkylamide sulfamate 0.25g / L

[0048] Magnesium Sulfate 10.0mM / L

[0049] Sodium dihydrogen phosphate-disodium hydrogen phosphate 5.0g / L

[0050] Mix the above reagents according to the indicated concentrations, and adjust the pH between 6.0 and 8.0 to prepare a test reagent for alanine aminotransferase. Using this reagent, through optical detection, a comparative experiment was carried out in the Laboratory Department of Beijing Red Cross Blood Center and Hitachi 7180 large-scale biochemical detector. The te...

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Abstract

An optical fast detection reagent preparation of alanine aminotransderase consists of an enzyme reaction reagent, a reductive color indicator, an agon coenzyme enzyme-accelerator, an organic surface active agent, an alkali metal stabilizer and a neutral buffer.

Description

technical field [0001] The invention relates to the technical field of detection reagents, belongs to medical clinical diagnosis, and is an optical rapid detection reagent for alanine aminotransferase. Background technique [0002] Alanine aminotransferase (ALT) is a non-plasma-specific enzyme that widely exists in various organs and tissues in the body, especially in the liver. When these tissue cells rich in alanine aminotransferase (ALT) are damaged, the release of alanine aminotransferase (ALT) from the cells increases, resulting in a rapid increase in the activity of alanine aminotransferase (ALT) in the blood, and the level of alanine aminotransferase (ALT) increases. Determination can reflect the damage of these tissues. [0003] Viral hepatitis has spread widely around the world and has seriously threatened human health. Elevated alanine aminotransferase has been generally considered to be one of the most sensitive indicators for clinical diagnosis of hepatitis act...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/48C12Q1/48
Inventor 何保山蔡新霞周爱玉岳伟伟李华清刘春秀
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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