Reagent formula for fastly inspecting glutamic-pyruvic transaminase by optical method
An alanine aminotransferase and reagent technology, which is applied in biological testing, material analysis and material inspection by observing the impact on chemical indicators, etc., can solve the problems of reagent interference factors, large errors, and difficulty in promotion and popularization.
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[0036] A reagent was prepared from the concentrations indicated for the following compounds:
[0037] Compound Concentration
[0038] L-alanine 90.0mM / L
[0039] α-ketoglutarate 4.0mM / L
[0040] Sodium Phosphate 30mM / L
[0041] Pyruvate oxidase 300.0U / mL
[0042] Peroxidase 600.0U / mL
[0043] 4-Aminoantipyridine 40.0mM / L
[0044] Thiamine pyrophosphate 0.11mM / L
[0045]Flavin adenine dinucleotide 0.30mM / L
[0046] 5'-Pyridoxal phosphate 0.50mM / L
[0047] Sodium alkylamide sulfamate 0.25g / L
[0048] Magnesium Sulfate 10.0mM / L
[0049] Sodium dihydrogen phosphate-disodium hydrogen phosphate 5.0g / L
[0050] Mix the above reagents according to the indicated concentrations, and adjust the pH between 6.0 and 8.0 to prepare a test reagent for alanine aminotransferase. Using this reagent, through optical detection, a comparative experiment was carried out in the Laboratory Department of Beijing Red Cross Blood Center and Hitachi 7180 large-scale biochemical detector. The te...
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