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Method for preparing mannosan

A mannan, acid method technology, applied in the field of mannan preparation, can solve the problems of reducing protein content, low product yield, large waste, etc. Effect

Inactive Publication Date: 2009-12-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a treatment step can reduce the protein content in the resulting product, and the mannan content is also guaranteed; the disadvantage is that ultrasonic treatment is used in the initial step, due to the toughness of the yeast cell wall, the wall breaking efficiency is not high, and finally Product yield is also low
In the extraction process of mannan, a large amount of organic substances are used, which is difficult to remove completely and poses a challenge to industrial production
In the traditional extraction process of β-1,3 / 1,6-glucan in yeast cell wall, mannan is listed as waste, which is a huge waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0029] 1. Preparation of mannan

[0030] The preparation procedure of mannan is as follows: figure 1 As shown, the specific steps are as follows:

[0031] 1. Enzymatic hydrolysis of yeast cell walls: Mix 100g of yeast cell walls (purchased from Hubei Yichang Angel Yeast Co., Ltd.), 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, at 37°C Stir with a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory, Puma brand adjustable speed stirrer, 200-300 rpm) for 30 minutes, and then at 3000 rpm (Shanghai Anting Scientific Instrument Factory) , DL6000B refrigerated centrifuge) centrifuged for 20 minutes to remove the supernatant to obtain 95.3g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0032] 2. Alkali treatment: 95.3 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast ...

Embodiment 2

[0050] Example 2, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0051] 1. Preparation of mannan

[0052] The preparation procedure of mannan is as follows: figure 1 As shown, the specific steps are as follows:

[0053] 1. Enzymolysis of yeast cell wall: Mix 50g of yeast cell wall, 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 250ml of water, and use a constant speed stirrer at a medium speed at 35°C (Shanghai Specimen Model Factory) , Huma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 60 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 25 minutes. Cleared to obtain 47.80g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0054] 2. Alkali treatment: add 47.80 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall into 500 ml of 0.5 M NaOH solution (that is, acc...

Embodiment 3

[0067] Example 3, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0068] 1. Preparation of mannan

[0069] The preparation procedure of mannan is as follows: figure 1 As shown, the specific steps are as follows:

[0070] 1. Enzymolysis of yeast cell wall: Mix 100g of yeast cell wall, 300mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, and use a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory) at 40°C , Puma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 30 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 20 minutes. Cleared to obtain 95.4g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0071] 2. Alkali treatment: 95.4 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall is added to 1000 ml of 1.0 M NaOH solution (i.e., acc...

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Abstract

The invention discloses a preparation method of mannan. The method comprises the following steps: 1) enzymatic hydrolysis and wall breaking treatment: adding lysozyme to the suspension of yeast cell wall, enzymatic hydrolysis at 35-40° C., and centrifuging to collect the precipitate; 2) alkali treatment: adding step 1) The obtained precipitate after enzymatic hydrolysis is stirred in NaOH solution at 75-90° C., and then centrifuged to collect the supernatant; 3) The supernatant obtained in step 2) is subjected to enzymatic treatment or acid treatment Finally, mix the obtained supernatant with CTAB solution with mass percentage concentration and sodium borate-sodium acetate buffer solution with mass percentage concentration of 0.3-0.8%, let stand for 24-48h, centrifuge, and carry out supernatant Mannan is obtained by drying; the method of the present invention is simple to operate, and each influencing factor is considered respectively to determine the preferred data range of parameters, so that the obtained mannan product has higher purity and yield, and the protein content and fat content in the product are less than 1%.

Description

technical field [0001] The invention relates to a preparation method of mannan. Background technique [0002] Yeast is a single-celled fungus and is the most widely used and economically valuable microorganism so far. The international FDA recognizes brewer's yeast as a safe microorganism and has been widely used in the production of beverages and foods. At present, my country consumes about 2,000 tons of yeast annually and produces about 3 tons of waste yeast sludge. Since the yeast cell wall is the main by-product of the beer manufacturing industry, and the main treatment method for it today is to sell it cheaply as roughage or discharge it directly, it causes a lot of waste of resources and pollution to the environment. [0003] Several research results have shown that pure yeast cell walls have obvious immune-boosting effects on animals, and also exhibit growth-promoting effects. Further evidence shows that the main mechanism is that the β-1,3 / 1,6-glucan and mannan co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C12P19/04
Inventor 呙于明张博
Owner CHINA AGRI UNIV