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Method for preparing mannosan

A mannan, acid method technology, applied in the field of mannan preparation, can solve the problems of reduced protein content, low product yield, large waste, etc. Effect

Inactive Publication Date: 2008-02-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a treatment step can reduce the protein content in the resulting product, and the mannan content is also guaranteed; the disadvantage is that ultrasonic treatment is used in the initial step, due to the toughness of the yeast cell wall, the wall breaking efficiency is not high, and finally Product yield is also low
In the extraction process of mannan, a large amount of organic substances are used, which is difficult to remove completely and poses a challenge to industrial production
In the traditional extraction process of β-1,3 / 1,6-glucan in yeast cell wall, mannan is listed as waste, which is a huge waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0029] 1. Preparation of mannan

[0030] The preparation operation process of mannan is shown in Figure 1, and the specific steps are as follows:

[0031] 1. Enzymatic hydrolysis of yeast cell walls: Mix 100g of yeast cell walls (purchased from Hubei Yichang Angel Yeast Co., Ltd.), 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, at 37°C Stir with a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory, Puma brand adjustable speed stirrer, 200-300 rpm) for 30 minutes, and then at 3000 rpm (Shanghai Anting Scientific Instrument Factory) , DL6000B refrigerated centrifuge) centrifuged for 20 minutes to remove the supernatant to obtain 95.3g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0032] 2. Alkali treatment: 95.3 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast c...

Embodiment 2

[0054] Example 2, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0055] 1. Preparation of mannan

[0056] The preparation operation process of mannan is shown in Figure 1, and the specific steps are as follows:

[0057] 1. Enzymolysis of yeast cell wall: Mix 50g of yeast cell wall, 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 250ml of water, and use a constant speed stirrer at a medium speed at 35°C (Shanghai Specimen Model Factory) , Huma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 60 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 25 minutes. Cleared to obtain 47.80g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0058] 2. Alkali treatment: add 47.80 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall into 500 ml of 0.5 M NaOH solution (that is, acco...

Embodiment 3

[0075] Example 3, the preparation of mannan and the preparation of water-insoluble β-1,3 / 1,6-glucan

[0076] 1. Preparation of mannan

[0077] The preparation operation process of mannan is shown in Figure 1, and the specific steps are as follows:

[0078] 1. Enzymolysis of yeast cell wall: Mix 100g of yeast cell wall, 300mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, and use a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory) at 40°C , Puma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 30 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 20 minutes. Cleared to obtain 95.4g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0079] 2. Alkali treatment: 95.4 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall is added to 1000 ml of 1.0 M NaOH solution (i.e., acco...

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Abstract

The invention discloses a method for preparing mannan. The method has the steps as follow: 1) enzyme hydrolysis and cell wall breaking process: adding lysozyme into yeast cell wall suspending liquid for enzymolysis, centrifugation and collection at a temperature between 35 to 40 DEG C; 2) alkali process: putting the deposition achieved from step 1) into the NaOH solution, and stirring, and then centrifugally collecting the supernatant fluid at a temperature between 75 to 90 DEG C; 3) enzymatic treating or acid treating the supernatant fluid achieved from step 2), and uniformly mixing the treated supernatant fluid with CTAB solution of concentration expressed in percentage by quality and sodium borate-sodium acetate buffer solution of 0.3 to 0.8 percent, and laying aside for 24 to 48 hours, and centrifuging, and drying the supernatant fluid to achieve mannan. The method mentioned in the invention is simple in control, and can determine the preferred data range by considering every influencing factors to ensure the mannan product has high purity and production, and the product has the protein content and fat content less than 1%.

Description

technical field [0001] The invention relates to a preparation method of mannan. Background technique [0002] Yeast is a single-celled fungus and is the most widely used and economically valuable microorganism so far. The international FDA recognizes brewer's yeast as a safe microorganism and has been widely used in the production of beverages and foods. At present, my country consumes about 2,000 tons of yeast annually and produces about 3 tons of waste yeast sludge. Since the yeast cell wall is the main by-product of the beer manufacturing industry, and the main treatment method for it today is to sell it cheaply as roughage or discharge it directly, it causes a lot of waste of resources and pollution to the environment. [0003] Several research results have shown that pure yeast cell walls have obvious immune-boosting effects on animals, and also exhibit growth-promoting effects. Further evidence shows that the main mechanism is that the β-1,3 / 1,6-glucan and mannan co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12P19/04
Inventor 呙于明张博
Owner CHINA AGRI UNIV