Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

R. grahami rnmunoregulating polypeptide, gene and variant and its application in medicine production

An immunomodulatory and variant technology, applied in the field of biomedicine, to achieve the effect of obvious tumor inhibition, convenient artificial synthesis and simple structure

Inactive Publication Date: 2007-09-19
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The application of amphibian drugs has a long history in my country, but the research on their active ingredients and pharmacological properties mainly focuses on small organic molecules such as alkaloids, and there are few researches on their skin active protein peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0030] Cloning of Immunomodulatory Peptide Gene of Frog Frog:

[0031] I. Extraction of total RNA from the skin of the fingerless frog:

[0032] A. The live fingerless frog is cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, get the skin tissue, weigh, get 300mg skin tissue, add 10ml total RNA extraction buffer (Trizol solution, U.S. GIBCOBRL company product), Homogenize for 30 minutes in a 20ml glass homogenizer.

[0033] B. Add an equal volume of phenol / chloroform solution, mix vigorously, leave at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0034] C. Add an equal volume of isopropanol to the supernatant, leave it at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, wash the precipitate onc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to an Odorrana grahami immunomodulation peptide, gene and variant and the application in the pharmacy belonging to the biomedicine field. The Odorrana grahami immunomodulation peptide is a circular peptide with a molecular weight of 1791.12 D and a isoelectric point of 9.84. The whole sequence of the Odorrana grahami immunomodulation peptide is Thr Ser Arg Cys Tyr Ile Gly Tyr Arg Arg Lys Val Val Cys Ser (TSRCYIGYRRKVVCS), wherein the fourth cysteine and 14th cysteine form intramolecular disulfide bond. The code gene is composed of 307 nucleotides, wherein the code mature parts are from 141th to 186th nucleotide. The produced variant is produced by substituting the fourth amino acid in the original sequence of the Odorrana grahami immunomodulation peptide. The artificial synthetic Odorrana grahami immunomodulation peptide and variant thereof has a strong immunomodulation activity and tumor resistance activity and can be used for immunomodulation, tumor treatment and chemotherapeutics. The invention has the advantages such as simple sequence and convenient synthesis.

Description

Technical field: [0001] The invention provides Rana grahami immunoregulatory peptide, its gene and variant and its application in pharmacy, belonging to the technical field of biomedicine. Background technique: [0002] In traditional Chinese medicine and ethnic medicine in China, many amphibians are widely used as medicinal materials, such as Bufo gargarizans, Bombina maxima, Pelophylax nigromaculata, Marsh frog ( Hylaranaguentheri) and the frog (Euphlyctislimnocharis) and so on. The skin and internal organs of these amphibians have a wide range of pharmacological activities and clinical curative effects. The reported pharmacological activities include: broad-spectrum antibacterial effect, anti-tumor, local anesthesia, analgesia, immune regulation, and effects on the cardiovascular system. On the one hand, the complexity of traditional Chinese medicine ingredients and the limitations of their processing methods are also important reasons for the inability of active ingredi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/64C12N15/12A61K38/12A61P37/02A61P35/00
Inventor 赖仞李建许李东升宋玉竹
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com