Large pore gel carrier of polyacrylamide for immobilization cells and preparation method

Abstract

This invention discloses a method for preparing polyacrylamide macroporous gel carrier for immobilized cells. The method comprises: preparing a mixed solution of acrylamide monomer and N,N'-methylene bis-acrylamide monomer, adding NaCl into the mixed solution to saturation, transferring the mixed solution into a container for preparing gel carrier, uniformly adding NaCl powder to 2-5 cm above the solution surface, exhausting oxygen in the mixed solution and in the container, sealing the container, radiating with radioactive rays so that acrylamide monomer and N,N'-methylene bis-acrylamide monomer polymerize to obtain a gel, and heating the gel in a water bath to obtain polyacrylamide macroporous gel carrier for immobilized cells. The polyacrylamide macroporous gel carrier can be used in immobilization of microbes, such as a mixture of bacteria and fungi, and mixed bacteria for cellulose degradation.

Description

Technical field [0001] The invention relates to an immobilized cell carrier and a preparation method thereof, in particular to a polyacrylamide macroporous gel immobilization material for fixing microbial cells and a preparation method thereof. Background technique [0002] Immobilized enzyme technology is a technology that connects enzymes to a certain solid-phase carrier to become immobilized enzymes through physical or chemical methods, while immobilized cell technology is a biological immobilization developed on the basis of immobilized enzyme technology The technology is to connect intact cells to a solid carrier, which not only eliminates the procedure of extracting enzymes from broken cells, but also maintains the integrity and activity of the enzymes. Compared with the immobilized enzyme technology, the immobilized cell technology can not only maintain the original state of the enzyme and enhance the stability, but it is also a multi-enzyme system without the need for coe...

AI Technical Summary

Problems solved by technology

However, the polymerization and cross-linking process in the preparation technology of frozen polyacrylamide carrier is spatially inhomogeneous, which leads to the inhomogeneity of the gel pore size and porosity, and the technology has strict requirements on temperature conditions. The lower the reaction temperature, the smaller the pore size and the lower the mass transfer efficiency; on the contrary, the higher the polymerization temperature, the larger the pore size and the lower the strength of the carrier

Therefore, the operation of preparing immobilized cell carriers by freezing technology is relatively difficult, and it is difficult to carry out large-scale preparation and application

Based on the current research, the gel obtained by chemical polymerization or radiation polymerization of acrylamide monomers generally has a pore size below 0.5 μm, while bacteria with cellulose degrading ability have a diameter of more than 1 μm, and fungal mycelium is longer. And most of them secrete intracellular enzymes, and bacterial cellulase is located on the cell membrane, and the bacteria need to attach to the cellulose to degrade it, so the immobilized bacteria prepared according to the traditional method cannot directly degrade cellulose

Therefore, the existing preparation method of immobilized cell carrier has inhibitory effect on the cell activity, the operation process is difficult to control precisely, and because the carrier pore size is too small, it will also hinder the technical problems such as contact between the cell and the substrate, especially for cellulose The cell immobilization technology of degrading bacteria is not yet mature