Protecting bioactive food ingredients using microorganisms having reduced metabolizing capacity
A bioactive, living microorganism technology, applied in the field of using microorganisms with reduced metabolic capacity to protect bioactive food ingredients, can solve problems such as loss of benefits of lactic acid starter, large special investment, and complicated process.
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Embodiment 1
[0127] Example 1: Utilizing the Bioactive Component of Interest Without Administration of the Claimed Invention
[0128] 1.1) Contained in the hydrolyzate Lactium bioactive peptide αS 1 Examples of [91-100]
[0129] When ingredients of peptide or protein type are added during the step of preparing the dairy "mixture" (powdering the milk), before hygienic heat treatment (i.e. 95°C, 8 minutes) and thus before fermentation, these ingredients are used, usually provided in powder form Easier and more convenient. In this case, the risk of metabolically active peptides is high. For example, using functional ingredients such as Lactium (Ingredia, France).
[0130] Procedure: Preparation of medium by hydrolysis of 120g / L skim milk powder supplemented with 1.5g / L Lactium The ingredients (corresponding to about 30 mg / L bioactive peptide αS1[91-100]) were then pasteurized at 95°C for 8 minutes.
[0131] Lactic acid starter was added to a ratio of 0.02%, and fermentation was c...
Embodiment 2
[0164] Example 2: Utilization of a Bioactive Component of Interest by Administration of the Claimed Invention
[0165] Based on industrial starters that do not consume peptide αS 1 [91-100] ability to screen industrial starter cultures. Of the 30 starters tested, all but one starter (the starter containing the only strain I-2774 which was microbiologically atypical) and formate Essentially all peptide αS is consumed during fermentation 1 [91-100].
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