Protecting bioactive food ingredients using microorganisms having reduced metabolizing capacity
A technology of biological activity and food, applied in the direction of microbes, microbes, methods of using bacteria, etc., can solve the problems of losing the benefits of lactic acid starter, complicated process, complicated industrial production line, etc.
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Embodiment 1
[0128] Example 1: Without the application of the claimed invention, utilizing the bioactive ingredient of interest 1.1) contained in the hydrolyzate bioactive peptide αS 1 Examples of [91-100] are used when adding peptide or protein type ingredients during the step of preparing a dairy "mixture" (powdering the milk), before hygienic heat treatment (i.e. 95°C, 8 minutes) and thus before fermentation. Often these ingredients are available in powder form for simpler convenience. In this case, the risk of metabolically active peptides is high. For example, using a functional ingredient containing a bioactive peptide (fragment 91-100 of casein αS1) such as (Ingredia, France).
[0129] Procedure: Preparation of medium by hydrolysis of 120g / L skim milk powder supplemented with 1.5g / L The ingredients (corresponding to about 30 mg / L bioactive peptide αS1[91-100]) were then pasteurized at 95°C for 8 minutes.
[0130] Lactic acid starter was added to a ratio of 0.02%, and ferment...
Embodiment 2
[0165] Example 2: Utilization of a Bioactive Component of Interest by Administration of the Claimed Invention
[0166] Based on industrial starters that do not consume peptide αS 1 [91-100] ability to screen industrial starter cultures. Of the 30 starters tested, all but one starter (the starter containing the only strain I-2774 which was microbiologically atypical) and formate Essentially all peptide αS is consumed during fermentation 1 [91-100].
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