Method for constructing tissue-engineering bone by pouring stem cell of inducing bone marrow ground substance into vivo

A technology of tissue engineering bone and stem cells, applied in prosthetics, bone implants, medical science, etc., can solve the limitations of clinical application, the difficulty of clinical application in the effectiveness and safety of gene therapy, and the adverse effects of cells on the coordinated release mechanism of multiple factors, etc. problems, to achieve the effect of promoting rapid vascularization, promoting the speed and quality of osteogenesis

Inactive Publication Date: 2007-12-26
戴江华
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Problems solved by technology

[0003] Growth factors play a vital role in the proliferation, migration, secretion, and differentiation of seed cells, and the current commonly used cell growth factor carrier slow-release system has the effects of sudden release, sustained and stable release of the carrier, and decomposition products produced during the degradation process. Unresolved issues such as adverse effects on cells and mechanisms for coordinated release of multiple factors
Although tissue engineering research has reached the gene level, it is difficult to go to clinical application due to the common effectiveness and / or safety issues of gene therapy
[0004] In conclusion, key technical problems such as vascularization of large tissue-engineered bone and sustained and stable release of growth factors have not been found so far, which severely limits their clinical application.

Method used

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Embodiment Construction

[0021] Taking this method to repair the large segmental bone defect of goat tibial shaft as an example

[0022] 1. Cultivation and expansion of goat BMSCs: extract goat autologous bone marrow respectively, use 1% pentobarbital sodium 0.3ml / kg intravenous anesthesia, sumaamp, atropine intramuscular injection compound anesthesia. After skin preparation at the bilateral iliac crests, 10ml of red bone marrow was extracted by puncturing with a 16-gauge bone needle under strict aseptic conditions. 2:1 serum-free medium, centrifuge at 1000r / min for 10min, discard the supernatant, wash 2-3 times with PBS, remove the upper layer of fat and tissue fluid, replace the lower cell layer (mixture containing BMSCs) with complete medium Resuspend, inhale into a petri dish, add complete medium, and culture in a 37°C, 5% CO2, saturated humidity incubator. Cultivate for about 2-3w, confluent into a monolayer, digest with a mixture of 0.25% trypsin and 0.02% EDTA, terminate the digestion, then pa...

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Abstract

The present invention relates to a method capable of utilizing internal perfusion to induce bone marrow matrix stem cell to construct tissue engineering bone. Said method includes the following steps: (1), extraction, separation and culture of bone marrow matrix stem cell; (2), preparation of coral composite artificial bone; and (3), creation of perfusion system.

Description

technical field [0001] The invention belongs to a method for reconstructing organs by tissue engineering in biomedical engineering, and specifically relates to a treatment method for constructing tissue-engineered bone by perfusing induced bone marrow stromal stem cells in vivo to repair large segmental bone defects, femoral head necrosis and other large bone defects. Background technique [0002] Currently, tissue engineering products that can be used clinically are mainly limited to several types of tissue engineering products for skin and cartilage, with very limited varieties and quantities, and relatively simple functions and structures. The most common clinical long bone defect is a difficult and hot issue that has plagued clinical medicine for a long time, and its repair and reconstruction have naturally become one of the most important directions in the field of bone tissue engineering research. However, the in vivo osteogenesis process of large tissue-engineered bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/02A61F2/28
Inventor 戴江华罗军戴闽
Owner 戴江华
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