Glucoside type flavone biological transformation and purification technique

Abstract

A process concerned with aglycon-anthoxanthin bio-conversion and purification is carried out by: dissolving anthoxanthin, regulating pH and temperature, adding enzyme A, B, C sequentially and acting a period respectively, concentrating, separating, adsorbing with macro-porous resin column and adsorbing, and concentrating and drying to obtain final product. The process has advantages such as moderate reaction conditions, less by-products and environment pollution, with higher value and without steric effect.

Description

(1) Technical field

[0001] The invention relates to a biological technology, in particular to a biotransformation and purification technology of aglycon type flavonoids. (2) Technical background

[0002] The metabolic pathways of flavonoids in animals are different. Only aglycon-type flavonoids can be directly absorbed into the blood of animals, while most glycoside-type flavonoids cannot enter the blood through the small intestine wall in the human body. Under the action of colonic microorganisms, some flavonoids can be hydrolyzed into aglycones before entering the blood. Therefore, the bioavailability of flavone glycosides in animals is much lower than that of aglycone flavones. Therefore, improving the configuration of flavonoids and increasing their absorption rate in blood is an important way to increase the bioavailability of flavonoid products. At present, there are mainly chemical and biological methods for the configuration transformation of flavone glycosides. Ma...