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Storage method for melanin cell suspension

A technology of melanocytes and preservation methods, applied in the preservation of human or animal bodies, animal cells, vertebrate cells, etc., can solve the problems of limited cell influence, non-adherent cell research, etc., to reduce cell aggregation and achieve significant benefits Effect

Inactive Publication Date: 2010-05-12
IND TECH RES INST
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Problems solved by technology

[0008] However, current knowledge about the effects of non-cryopreservation methods on cells is limited. Among them, studies on the effects of low temperature environments on adherent cells are limited to adherent cells adhered to culture dishes, and have not been studied in suspensions. adherent cells

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  • Storage method for melanin cell suspension
  • Storage method for melanin cell suspension
  • Storage method for melanin cell suspension

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Experimental program
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Embodiment Construction

[0028] experiment process

[0029] figure 1 It is the general process of the melanocyte preservation experiment according to the preferred embodiment of the present invention. In order to test the effect of storage temperature and time on the viability and survival rate of melanocytes, the skin melanocytes (dermal melanocytes) are obtained and cultured from neonatal foreskin or adult epidermal tissue, and then prepared into a suspension (step 102 ). Next, after storing at different temperatures for a period of time (step 104), test the cell activity and survival rate (step 106), and observe the cell aggregation.

[0030]In this embodiment, the Thiazolyl blue colorimetric method and the cell counting method are respectively used for testing. Tetrazolium salt colorimetric method is a method commonly used in biology to determine cell survival or proliferation, which mainly relies on the action of succinate dehydrogenase in the mitochondria of living cells, and the tetrazolium s...

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Abstract

The invention relates to a preservation method for a melanocyte. The melanocyte can be preserved at a temperature ranging from 4 to 22.5 DEG C after being made into suspension, wherein the best preservation temperature of the suspension liquid of the melanocyte of newly born baby ranges from 4 to 15 DEG C, a cell existence rate and activity can be maintained above 71 percent after the melanocyte is preserved for 48 hours; the best preservation temperature of the suspension liquid of the melanocyte of adult ranges from 10 to 22.5 DEG C, cell existence rate and activity can be maintained above 72 percent after the melanocyte is preserved for 24 hours. Furthermore, the aggregate of melanocyte can be avoided at the above temperature range. The suspension liquid of the melanocyte can be used for the treatment of leukoderma, and other symptom caused by lack of melanocyte.

Description

technical field [0001] The present invention relates to a preservation method of an adhesive cell suspension, more specifically, a preservation method of a melanocyte suspension. Background technique [0002] Vitiligo in albinism (Leukoderma) is a macular pigmentation loss disease, which occurs in about 1% to 2% of the population, and its clinical symptoms are irregular white patches on the skin or mucous membranes, Vitiligo Almost all melanocytes on the affected area are absent. In terms of the treatment of leukoplakia, in addition to dyeing and covering with dyes or cosmetics, local application of steroid preparations or skin grafting can also be used for treatment. However, these methods are not suitable for large areas and may cause Side effects such as skin atrophy. [0003] At present, the most commonly used method for treating leukoplakia is the combined treatment of oral or external photosensitizers (such as Psoralen) and long-wavelength ultraviolet light (UVA), or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61K35/12A61P17/00A01N1/02
Inventor 施冰如廖智菁申屠子萍杨莹蓉吴怡萍
Owner IND TECH RES INST