Micro-zoology preparations for feeding
A micro-ecological preparation and feeding technology, which is applied in the field of feed industry and animal husbandry, can solve the problems of single function and poor adaptability of animal digestive tract environment, achieve strong adaptability, promote animal growth and improve immunity , the effect of strong inhibitory ability
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Embodiment 1
[0020] 1. Preservation status of the three bacteria:
[0021] Bacteria D, E, and F have been preserved in the China Center for Type Culture Collection, and the preservation date is March 16, 2007.
[0022] CCTCC deposit number
Strain number
strain name
CCTCC M 207096
CCTCC M 207097
CCTCC M 207098
Strain D
strain E
Strain F
Lactobacillus delbrueckii
Strange Yeast (Saccharomyces paradozus)
[0023] Lactobacillus delbrueckii belongs to Lactobacillus, Bacillus subtilis belongs to Bacillus; Saccharomyces mirabilis belongs to yeast.
[0024] 2. Morphological, physiological, biochemical and metabolic characteristics of bacteria D, E, and F:
[0025] Morphological, physiological, biochemical and metabolic characteristics of strain D
[0026] Test items
result
Test items
result
Test items
result
s
cell shape
endo...
Embodiment 2
[0037] Others are with embodiment 1. Take a ring of bacterial lawn from the inclined surface of the test tube to inoculate D, E, and F bacteria into 50ml MRS liquid medium, 50ml broth liquid medium, and 50ml potato dextrose liquid medium, and culture at a constant temperature of 37°C for 21 hours. The suspension was mixed in a volume ratio of 2:1:2 (D bacteria: E bacteria: F bacteria), and added to the sterilized culture medium with brewer's grains as the main raw material in a ratio of 12% (V / W). Mix evenly, then ferment at 32°C for 42 hours, and then freeze-dry.
[0038] The physical and chemical characteristics and biological properties of the final product: yellowish brown, powdery; water content ≤ 10%; total number of beneficial bacteria 9 × 108 CFU / g~1×10 10 CFU / g, of which D bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, E bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, F bacteria content is 1×10 8 CFU / g~1×10 10 CFU / g.
Embodiment 3
[0040] Others are with embodiment 1. Take a ring of bacterial lawn from the inclined surface of the test tube to inoculate D, E, and F bacteria into 50ml MRS liquid medium, 50ml broth liquid medium, and 50ml potato dextrose liquid medium, and culture at a constant temperature of 37°C for 24 hours. The suspension was mixed at a volume ratio of 1:1:2 (D bacteria: E bacteria: F bacteria), and added to the sterilized culture medium with brewer's grains as the main raw material in a ratio of 15% (V / W). Mix evenly, then ferment at 34°C for 48 hours, and then freeze-dry.
[0041] The physical and chemical characteristics and biological properties of the final product: yellowish brown, powdery; water content ≤ 10%; the total number of beneficial bacteria containing 9 × 10 8 CFU / g~1×10 10 CFU / g, of which D bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, E bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, F bacteria content is 1×10 8 CFU / g~1×10 10 CFU / g.
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