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Method for preparing centipede polysaccharide protein composition with anti-tumor active and use

The technology of centipede polysaccharide and compound is applied in the fields of biochemical pharmacy and natural polymer, and achieves the effect of simple and easy production process, high quality and simple and easy purification process.

Inactive Publication Date: 2008-04-30
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 2. There are many methods and related drugs for treating tumors, but they all have certain limitations
[0008] 4. Research progress and research gaps of centipede in Chinese medicine
However, so far, no preparation method and application of centipede polysaccharide protein complexes with anti-tumor activity have been disclosed or used.

Method used

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  • Method for preparing centipede polysaccharide protein composition with anti-tumor active and use
  • Method for preparing centipede polysaccharide protein composition with anti-tumor active and use
  • Method for preparing centipede polysaccharide protein composition with anti-tumor active and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of centipede polysaccharide protein complex (SPPC)

[0040] ①Take 100g of fresh adult centipede, wash 3 times, and dry at 60°C for 2 hours;

[0041] ② grind the dried raw material matrix in the first step and pass through a 50-mesh sieve to obtain centipede fine powder;

[0042] ③Extract the centipede fine powder in the second step with 500ml distilled water at 80°C for 1 hour, filter it through gauze (to obtain an extract), take the residue and repeat the extraction three times with 400ml distilled water (to obtain three extracts), and combine the above Four extracts were centrifuged at 2500r / min for 20 minutes to obtain the supernatant;

[0043] ④ Concentrate the supernatant in step 3 under reduced pressure at 50°C, add 5 times the volume of 95% ethanol, let it stand overnight at 4°C, pour off the supernatant, collect the precipitate, and centrifuge at 2500r / min for 10 minutes to collect the precipitate;

[0044] ⑤ Dissolve the precipit...

Embodiment 2

[0047] Embodiment 2: the preparation of centipede polysaccharide protein complex (SPPC)

[0048] ①Take 100g of fresh adult centipede, wash it 4 times, and dry it at 70°C for 3 hours;

[0049] ② Grinding the dried raw material matrix in the first step and passing through a 100-mesh sieve to obtain centipede fine powder;

[0050] ③Extract the centipede fine powder in the second step with 500ml distilled water at 85°C for 2 hours, filter it through gauze (to obtain an extract), take the residue and repeat the extraction three times with 400ml distilled water (to obtain three extracts), and combine the above Four extracts were centrifuged at 2500r / min for 25 minutes to obtain the supernatant;

[0051] ④ Concentrate the supernatant in step 3 under reduced pressure at 60°C, add 5 times the volume of 95% ethanol, let it stand overnight at 4°C, pour off the supernatant, take the precipitate, and centrifuge at 2500r / min for 10 minutes to collect the precipitate;

[0052] ⑤ Dissolve t...

Embodiment 3

[0055] Embodiment 3: the preparation of centipede polysaccharide protein complex (SPPC)

[0056] ①Take 100g of fresh adult centipede, wash 5 times, and dry at 70°C for 4 hours;

[0057] ② Grinding the dried raw material matrix in the first step and passing through a 200-mesh sieve to obtain centipede fine powder;

[0058] ③Extract the centipede fine powder in the second step with 500ml distilled water at 90°C for 3 hours, filter it through gauze (to obtain an extract), take the residue and repeat the extraction three times with 400ml distilled water (to obtain three extracts), and combine the above Four extracts were centrifuged at 2500r / min for 30 minutes to obtain the supernatant;

[0059] ④Concentrate the supernatant in step 3 under reduced pressure at 70°C, add 5 times the volume of 95% ethanol, let it stand overnight at 4°C, pour off the supernatant, collect the precipitate, and centrifuge at 2500r / min for 10 minutes to collect the precipitate;

[0060] ⑤ Dissolve the p...

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Abstract

The invention discloses a process for preparation and a use of an antineoplastic and active scolopendra polysaccharide-protein composition. The steps are: <1>cleaning and drying a scolopendra with few thorns as parent material; <2> grinding dried parent material thereby getting scolopendra fines; <3> the scolopendra fines are aqueous extracted and filtered thereby getting residue which is then aqueous extracted repeatedly, and extract supernate after centrifuging; <4>decompress and condensed supernate, sediment is obtained after raising mellowly; <5> after the sediment is salted out segment through ammonium sulfate, 60% of saturated sediment is collected; <6> the sediment is concentrated after analytical column, elution and desalinization of DEAE-50 cellulose ion-exchange layer; <7>the scolopendra polysaccharide-protein composition is obtained after concentrated solution is purified by Sephadex G-200 column. The process for preparation is feasible, convenient in operation and wide in raw material resources. The invention is applied in antitumor agent, thereby improving immunity with notable curative effect. The polysaccharide-protein composition is applied in preparing treatment or the antitumor agent.

Description

technical field [0001] The invention belongs to the field of natural macromolecules, and also belongs to the field of biochemical pharmacy, and relates to a new natural anti-tumor drug, more specifically to a preparation method of a centipede polysaccharide protein complex with anti-tumor activity, and also to a kind of anti-tumor activity. Application of the centipede polysaccharide-protein complex in the preparation of medicines for treating or preventing tumors. Background technique [0002] 1. Tumor has become the number one killer threatening human health [0003] Tumor is one of the common and frequently-occurring diseases that seriously threaten human life, and its incidence rate is increasing year by year. According to the survey of the World Health Organization, nearly 10 million cancer patients are newly discovered every year in the world. Nearly 7 million people die from cancer every year. Recent statistics show that about 20 million cancer patients are newly d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00A61P1/00A61P1/16A61P15/14A61P17/00A61K35/648
Inventor 杨静陈刚周倩刘洋朱玲新李颖乐江敖英
Owner WUHAN UNIV
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