Basophilic bacillus cereus, produced interior contact dextranase and application of the same

A technology of endoglucanase and alkalophilic bacillus, applied in the biological field, can solve the problems of poor thermal ability, enzyme production ability and specific activity of strains, etc., to achieve stable enzyme activity, inhibit bacterial contamination, It is beneficial to the effect of large-scale fermentation production

Inactive Publication Date: 2008-05-07
SHENZHEN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, there are many deficiencies in the research on alkaline endoglucanase, such as low enzyme production ability and specific activity of the strain, or poor resistance to metal ions, surfactants, and heat.
At the same time, alkalophilic bacillus mainly produces some alkaline enzymes, so far there has been no report of alkalophilic bacteria producing neutral endoglucanase

Method used

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  • Basophilic bacillus cereus, produced interior contact dextranase and application of the same
  • Basophilic bacillus cereus, produced interior contact dextranase and application of the same
  • Basophilic bacillus cereus, produced interior contact dextranase and application of the same

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Embodiment Construction

[0040] The present invention will be further described in detail below in conjunction with the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0041] (1) Materials

[0042] 1. Strains

[0043] Bacillus sp.III-3 is an endoglucanase-producing strain screened from paper mill alkaline sludge. Culture preservation unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms, preservation date: August 22, 2007, preservation number: CGMCC No.2138.

[0044] 2. Culture medium (mass percentage)

[0045] Incline medium: 1% soluble starch, 0.5% compound peptone, 0.5% yeast powder, 0.1% KH 2 PO 4 , 0.02% MgSO 4 ·7H 2 O, 1% NaCl, 0.5% Na 2 CO 3 (separately sterilized), 1.5% agar.

[0046] Liquid fermentation medium: 1% compound peptone, 0.5% yeast powder, 0.5% NaCl, 0.1% KH 2 PO 4 , 0.25% Na 2 HPO 4 12H 2 O, 2.0% CMC-Na, 0.5% Na 2 CO 3 (separately sterilized).

[0047] 3. Mai...

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Abstract

The invention discloses an alkaliphilic bacillus, in particular to an endoglucanase strain screened out from the alkaline sullage in the paper mill. The invention also discloses a neutral endoglucanase and an alkaline endoglucanase. The two endoglucanases can be applied in the detergent and textile industries. The alkaliphilic bacillus belongs to alkaliphilic extremophiles. Compared with the prior industrial strains, the invention can be cultured under the condition of high alkalinity, which has the advantages that mixed fungi pollution can be effectively inhibited and large-scale fermentation production is favored.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an alkalophilic bacillus and the endoglucanase produced by it and application thereof. Background technique [0002] Endoglucanase (β-1, 4-endoglucanase, Endoglucanase / Endo-1, 4-β-Glucanase, EC3.2.1.4) is an important component of cellulase enzyme system, which can The cellulose molecule is cut from the inside at the β-1,4 glycosidic bond. According to the optimal pH value of the catalytic reaction, endoglucanase can be divided into acidic endoglucanase (optimum pH 3-5), neutral endoglucanase (optimum pH 6-8) and alkali Sexual endoglucanase (optimum pH 8 ~ 10). Acid endoglucanase is mainly produced by filamentous fungi, and its producing bacteria mainly include Trichoderma reesei, Trichoderma konningen, and Aspergillus niger. The study of acid endoglucanase was the earliest. However, with the continuous expansion of the scope of industrial application and the deepening...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C11D3/386C12R1/07
Inventor 邢苗刘森林陈伟钊
Owner SHENZHEN UNIV
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