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Electrochemical assay

An electrochemical and electrode technology, applied in the field of assay kits of content or presence, which can solve the problems of ineffectiveness, low concentration of binding reagent-analyte complexes, difficulty in presence and/or content, etc.

Inactive Publication Date: 2008-05-21
INVERNESS SWITZERLAND GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a problem with this type of assay is that it is sometimes not very efficient at low analyte concentrations
This is because the concentration of the bound reagent-analyte complex will also be lower, and determining the presence and / or amount of the substance at lower concentrations may be difficult

Method used

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  • Electrochemical assay
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Experimental program
Comparison scheme
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Embodiment

[0124] 3.1 Design of scale-up carrier / phase

[0125] 3.1.1 UV-cleavable electrochemical molecules

[0126] Ortho-nitrobenzyl derivatives have been widely used in organic synthesis—especially as a protecting group, as well as in biological applications for the separation, purification and identification of target biomolecules, due to their use in low-energy UV It also has high photolysis efficiency when exposed to light.

[0127] A hypothetical photolysis mechanism of o-nitrobenzyl derivatives is shown in Scheme 3.1. This hypothetical mechanism is considered to be related to the ring form B Fast Equilibrium Acid Nitro Intermediate A Formed by a three-step process:

[0128] 1) UV light activates nitro

[0129] 2) Intramolecular hydrogen transfer from the benzylic carbon to the oxygen of the nitro group.

[0130] 3) Electronic rearrangement.

[0131] followed by compound D release of and nitroso derivatives C The formation of is achieved by oxygen transfer from nitrogen ...

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Abstract

A method of determining the presence or amount of analyte in a fluid sample, which comprises: (a) contacting a fluid sample with a binding reagent that comprises a plurality of cleavable species and wherein said species, when cleaved, are detectable using electrochemical means; (b) separating any binding reagent-analyte complex that forms from the unbound binding reagent; (c) cleaving the cleavable species from the immobilized binding reagent- analyte complex; and (d) detecting the cleaved species using electrochemical means.

Description

technical field [0001] The present invention relates to a method for determining the presence or amount of an analyte in a fluid sample, a binding reagent used in the method, the use of the binding reagent in an immunoassay and a method for measuring the amount or presence of an analyte in a sample kit. Background technique [0002] It is known that immunoassays for determining the presence or amount of an analyte in a fluid sample rely on the use of a binding reagent that binds the analyte of interest. In such devices a binding reagent-analyte complex is formed and then immobilized at a capture site, and the presence or amount of the analyte is determined. Such assays can be achieved by various methods, such as fluorometric assays. However, a problem with this type of assay is that it is sometimes not very efficient at low analyte concentrations. This is because the concentration of the binding reagent-analyte complex will also be lower, and determining the presence and / ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/532G01N33/5438G01N33/58
Inventor D·E·威廉斯P·洛C·J·斯莱文A-C·郝维S·J·卡莱尔A·汤姆森
Owner INVERNESS SWITZERLAND GMBH