Fixation cell (or enzyme) internal circulation fluidized bed reactor and application thereof in organic phase biological catalysis

A technology of fluidized bed reactor and immobilized cells, which is applied in the direction of supporting/immobilizing microorganisms, special-purpose bioreactors/fermenters, biochemical cleaning devices, etc., to achieve high oxygen dissolution efficiency and low shear force , the effect of low energy consumption

Inactive Publication Date: 2008-07-09
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the immobilized biological desulfurization process of oil involves a multiphase system consisting of gas phase, water phase, oil phase and biocatalyst solid phase, the existing fluidized bed reactor is not fully suitable for this system

Method used

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  • Fixation cell (or enzyme) internal circulation fluidized bed reactor and application thereof in organic phase biological catalysis
  • Fixation cell (or enzyme) internal circulation fluidized bed reactor and application thereof in organic phase biological catalysis
  • Fixation cell (or enzyme) internal circulation fluidized bed reactor and application thereof in organic phase biological catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] See Figure 1~3 According to the connection relationship of the aforementioned reactors, a circulating fluidized bed reactor in immobilized cells (or enzymes) that can be used as an organic phase biocatalytic reaction is produced.

[0042] The circulating fluidized bed reactor for immobilized cells (or enzymes) provided in this example has a height of 350 mm, a volume of 700 mL, and an effective volume of 500 mL. The lower part of the reactor shell is equipped with an external heat exchange jacket for cold and hot water to control the reaction temperature.

Embodiment 2

[0044] Batch immobilized cell biocatalysis for desulfurization reaction.

[0045] Using the circulating fluidized bed reactor in the immobilized cells (or enzymes) made in Example 1, the immobilized biocatalyst was used to remove sulfur in the simulated oil phase. The steps are as follows:

[0046] (1) Cultivation of biological desulfurization bacteria

[0047] Take 0.5 mL of glycerol-preserved Pseudomonas delafildii R-8 strain (Pseudomonas delafildii R-8, CGMCC NO.0570), add to several Erlenmeyer flasks containing 150 mL of culture medium, at 30°C, 170r Cultivate for 2 to 3 days under the condition of 1 / min; centrifuge to obtain R-8 cells, wash 2 to 3 times with normal saline, and suspend the obtained Pseudomonas delbruecki R-8 cells in normal saline, store in a refrigerator at 4°C placed in.

[0048] The composition of the medium is 2.44 g KH per liter of water 2 PO 4 , 14.03 g Na 2 HPO 4 12H 2 O, 0.4g MgCl 2 ·6H 2 O, 0.001g CaCl 2 2H 2 O, 0.001g FeCl 3 ·6H 2 O...

Embodiment 3

[0055] Continuous biocatalytic removal of sulfur from diesel with immobilized cells.

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Abstract

The invention relates to an air lift fluidized bed reactor for gas-liquid-solid heterogeneous biocatalysis, being especially applicable to an internal recycle fluidized bed reactor of immobilized enzyme or immobilized cell, which comprises a reactor casing, a gas nozzle, a conical internal flow guiding canister, a low opening distributor of the internal flow guiding canister and a reflux condenser, and is characterized in that the invention comprises the conical reactor casing, wherein the low end of the reactor tank bottom is the gas nozzle to form the conical fluidized bed, the conical internal flow guiding canister, wherein the low portion is the circular ring low opening distributor of the internal flow guiding canister, immobilized carrier particles are capable of good outflow via adjusting the channel angle and aperture of the low opening distributor, the conical reactor casing is opened with a light phase injection port, a weight phase injection port, a production vent and an air vent which is connected with the reflux condenser and the like. The reactor of the invention is an air lift type, internal recycle and conical fluidized bed reactor, which has the advantages of small shearing force, low energy consumption, rapid as-liquid-solid mass transfer, high solvent efficiency and easy magnification, being especially applicable to immobilized cell or enzyme organic phase biocatalysis reaction.

Description

technical field [0001] The invention relates to a novel air-lift fluidized bed biochemical reactor. More specifically, the reactor can be used for biocatalytic reactions of immobilized enzymes or immobilized cells, and is especially suitable for organic phase biocatalytic reactions of aerobic immobilized cells. Background technique [0002] The rapid development of biotechnology will play a key role in improving and improving the quality of human life, and has become one of the high-tech technologies with great development potential in the 21st century. Among them, biocatalysis and biotransformation are the main body of the new generation of industrial biotechnology. Biocatalysis refers to the use of biocatalysts (enzymes, microorganisms, and animal and plant cells) to catalyze the synthesis or decomposition of organic matter. It has the characteristics of high selectivity, mild reaction conditions, and environmental friendliness. However, when free cells or enzymes are us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M23/02C12M25/20C12M29/06
Inventor 刘会洲李玉光熊小超李望良安震涛邢建民李信
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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