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Saccharomyces cerevisiae CGMCC No.2266 and its application in preparation of (S)-(-)- beta-hydroxyphenyl propionic acid ethyl

A technology of ethyl carbonyl phenylpropionate, cgmccno.2266, applied in Saccharomyces cerevisiae CGMCC No.2266 and its application field in the preparation of (S)-(-)-beta-hydroxyphenylpropionate ethyl ester, can solve the problem of Low biotransformation production capacity, unbearable cell catalysis, cytotoxicity and other problems, to achieve the effect of easy large-scale industrial production, environmental friendliness and low cost

Inactive Publication Date: 2010-06-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extremely low solubility of the organic substrate β-carbonylphenylpropionate ethyl in water, the organic substrate will also cause certain toxicity to the cells, and the cell catalysis cannot tolerate a large amount of substrates, so the biotransformation production capacity is low

Method used

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  • Saccharomyces cerevisiae CGMCC No.2266 and its application in preparation of (S)-(-)- beta-hydroxyphenyl propionic acid ethyl
  • Saccharomyces cerevisiae CGMCC No.2266 and its application in preparation of (S)-(-)- beta-hydroxyphenyl propionic acid ethyl
  • Saccharomyces cerevisiae CGMCC No.2266 and its application in preparation of (S)-(-)- beta-hydroxyphenyl propionic acid ethyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Process flow chart see figure 1 .

[0041] Incline cultivation:

[0042] Inoculate CGMCC No.2266 bacteria into the slant culture medium and culture at 30°C for 4-6 days to obtain the slant bacteria. Slant medium preparation: 10g of wort juice, 3g of yeast powder, 5g of peptone, 10g of glucose, 20g of agar, 1000g of water, natural pH, sterilized at 121°C for 20 minutes, cooled to make a slope.

[0043] Seed culture and fermentation culture:

[0044] Both the seeds and the fermentation medium are liquid medium, prepared according to the following composition: glucose 30g, yeast powder 3g, ammonium sulfate 5g, anhydrous MgSO 4 0.25g, K 2 HPO 4 ·3H 2 O 1g, KH 2 PO 4 1g, 1000g water, natural pH, sterilized at 121°C for 20 minutes, cooled for later use. Use an inoculation needle to take a ring of slant bacteria from the slant medium and inoculate it into a 250ml Erlenmeyer flask containing 100ml of liquid medium, and culture it at 30°C and 180r / min for 24 hours to ...

Embodiment 2

[0050] CGMCC No.2266 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. 4 bottles of 100ml fermented broth with a dry weight of 430mg were mixed with 100ml of sodium alginate solution with a concentration of 2% (w / w%) to make a mixed solution, and the mixed solution was respectively packed into syringes with different needle sizes. Add 3.5% (w / w%) CaCl dropwise 2 Immobilized particles were formed in the solution, and the diameters of the formed particles were 2mm, 3mm, 4mm and 5mm respectively. They were solidified at 37°C for 30 minutes, and the obtained immobilized particles were washed with sterile physiological saline to remove excess calcium ions and untreated particles. For the captured cells, the obtained immobilized cell particles were continued to proliferate and culture in liquid medium for 24 hours.

[0051] Add the above-mentioned 4 kinds of immobilized cell particles that have been proliferated and cultured into 300ml...

Embodiment 3

[0055] CGMCC No.2266 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. Mix 100ml of 4 bottles of fermented liquid with a dry weight of 430mg of bacteria and 100ml of sodium alginate solution with a concentration of 2% (w / w%) to make a mixed solution, put the mixed solution into a syringe respectively, and drop in 3.5% (w / w%)CaCl 2 Form immobilized particles in the solution, the diameter of the formed immobilized particles is 2mm, solidify at 37°C for 30min, and the obtained immobilized particles are washed with sterile physiological saline to remove excess calcium ions and uncaptured cells, and the obtained 4 The bottle-immobilized cell particles continued to proliferate and culture in liquid medium for 0h, 24h, 48h and 72h respectively.

[0056] Add the above-mentioned 4 kinds of immobilized cell particles proliferated and cultured into 300ml dibutyl phthalate containing 15.45mmol ethyl β-carbonylphenylpropionate, and carry out ...

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Abstract

The invention provides a new microbial strain of vintage yeast CGMCC No.2266 and the application thereof in preparing (S)-(-)-beta-hydroxyphenyl ethyl propionate by microbial transformation. The vintage yeast CGMCC No.2266 is preserved in the China center of General Microbial Culture Collection, and the storage number is CGMCC No.2266, the storage date is 26 November 2007. The invention provides anew bacterial strain for biologically transforming beta-hydroxyphenyl ethyl propionate into (S)-(-)-beta-hydroxyphenyl ethyl propionate. The excess value of the (S)-(-)-beta-hydroxyphenyl ethyl propionate which is produced by the method of the invention on the enantiomer is more than 99.0 percent, the Mol transformation rate of the base material can reach 92.2 percent, the purity of the product can reach 99.8 percent, the Extraction yield of silica gel column chromatography product is 95.6 percent, and the fixed cell particles can be recycled for ten times.

Description

(1) Technical field [0001] The invention relates to a new microbial strain Saccharomyces cerevisiae CGMCC No. 2266 and its application in the preparation of (S)-(-)-beta-hydroxyphenylpropionate ethyl by microbial transformation. (2) Background technology [0002] (S)-(-)-β-hydroxyphenylpropanoic acid ethyl ester ((S)-(-)-Beta-hydroxy-benzenepropanoic acidethyl ester), the molecular formula is C 11 h 14 o 3 , molecular weight 194.23. Ethyl (S)-(-)-β-hydroxyphenylpropionate is an important intermediate in the synthesis of (S)-fluoxetine. The action time of (S)-fluoxetine in the human body is 3 times that of (R)-fluoxetine. The trade name of fluoxetine hydrochloride is Prozac, which is a selective serotonin reuptake inhibitor (SSRI), which can effectively inhibit the uptake of 5-serotonin from the synaptic cleft by neurons, and increase the availability of serotonin in the gap. This neurotransmitter is used to improve emotional state and treat depressive mental disorders. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N11/04C12P7/62C12R1/865
CPCY02P20/50
Inventor 欧志敏杨根生徐姜炎
Owner ZHEJIANG UNIV OF TECH
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