Unlock instant, AI-driven research and patent intelligence for your innovation.

Non-contact type photoelectricity pH value detection method and used sensor

A non-contact, detection method technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve problems such as the inability to effectively meet the development needs of bioreactor technology. , to achieve the effect of simple installation, low energy consumption and small size

Inactive Publication Date: 2010-12-15
INST OF MECHANICS CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing measurement technology cannot effectively meet the technical development needs of bioreactors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-contact type photoelectricity pH value detection method and used sensor
  • Non-contact type photoelectricity pH value detection method and used sensor
  • Non-contact type photoelectricity pH value detection method and used sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Such as figure 1 As shown, the sensor detection part includes silicone infusion tube 1, probe fixture 2, transmitting optical fiber 3, receiving optical fiber 4, reflecting surface 5, diode 6, diode 7, photosensitive element 8, solution to be tested 9, optical fiber connector 10, probe17. Before testing, clamp the silicone infusion tube 1 containing the liquid to be tested 9 in the deep groove provided on the probe fixture 2, and fix the probe 17 so that the probe 17 is close to the outer wall of the silicone infusion tube 1, and the probe is clamped. A reflective surface 5 is provided on the tool 2 at a position opposite to the probe 17 . When working, the sensor control unit first controls the diode 6 to emit green light with a center wavelength of 565nm, and the green light passes through the emitting fiber 3 and the probe 17 and then transmits the silicone infusion tube 1 containing the liquid to be tested 9, and transmits the transmitted light through the reflecti...

Embodiment 2

[0052] In this embodiment, the liquid to be tested in the petri dish can be directly detected, such as figure 2 As shown, a jig 12 is arranged on the outside of the culture dish 11, the transmitting optical fiber 13 is clamped on one side of the jig 12 through the probe 15, and the receiving optical fiber 14 is clamped on the opposite side of the jig 12 through the probe 16, so that Probe 15 and probe 16 are opposite, so that the effective transmission of optical fiber; During work, also can measure and obtain level signal V according to the work step in embodiment 1 a and V b , into the preset formula pH=a×(V a ÷V b )+b, the pH value of the liquid 9 to be tested can be obtained.

[0053] The sensor of the present invention can verify the anti-interference performance of the present invention through a test method. Prepare pH before the experiment and be respectively the phosphate buffer solution of 6.67, 7.01, 7.32, 7.67 and 7.96, all add 3.9mg / L phenol red; Adopt the st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a non-contact photoconductive pH value test method and the sensor thereof. Using the method can timely and quantificationally measure the pH value of culture fluid in a bioreactor, thereby providing accurate and reliable data for the culture condition monitoring of the bioreactor; the volume of the sensor implementing the teat method is small, the energy consumption is little, the installation is simple, and the capacity of resisting disturbance is strong; during the detection, the method fully avoids the influences of the pollution on the solution to be tested withoutdirectly contacting the solution to be tested.

Description

technical field [0001] The invention relates to a non-contact photoelectric pH value detection method and a sensor for implementing the method. Background technique [0002] In bioreactors, the pH value of the working fluid needs to be monitored in real time. For example, the pH value of the cell culture solution is easy to change in different gas environments, and the metabolism of the cells will also change the pH value of the culture solution. Usually, cells need to grow normally under a certain pH value of the solution, so monitoring the pH value of the working liquid is an important condition for the normal operation of this type of reactor. 3.9 mg / L of phenol red is dissolved in the commonly used biological culture solution. The phenol red is light yellow in acidic environment and purple in alkaline environment. Phenol red is used as an indicator to show color. The color change was used to assess the pH of the culture medium. [0003] The experimenter can know the a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/80
Inventor 龙勉李振涵高宇欣孙树津
Owner INST OF MECHANICS CHINESE ACAD OF SCI