Kit and method for quantitative determination of Fumonisin B1 content in food by ELISA detection technique

Abstract

The invention relates to a reagent box for quantificationally detecting the content of fumonisin B1 in food by adopting an enzymoimmunoassay technology and the detecting method thereof. The reagent box includes the steps that a fumonisin B1-KLH antigen is prepared; a polyclonal fumonisin B1 antibody is prepared; an enzyme labeling antigen is prepared; a coated plate is prepared, and a reagent is prepared. The invention mainly adopts the enzymoimmunoassay technology to detect the fumonisin B1, can realize the aim of quantificationally detecting the content of fumonisin B1 in food and alimentary crops; and the reagent box has simple structure, convenient usage, cheap price and high sensitivity; the detecting method has strong specificity, high sensitivity, good veracity, and the sensitivity can reach gamma / kg grade.

Description

technical field [0001] The invention belongs to the technical field of food immune analysis, and relates to the detection of the content of fumonisin B1 in food and grain crops, in particular to a kit for quantitatively detecting the content of fumonisin B1 in food by enzyme-linked immunoassay technology and its detection method. Background technique [0002] Fumonisins (Fumonisins, FBs) are a group of structural analogs that were first isolated from the culture of F. verticilliodes (synonym: F. moniliforme Sheldon) MRC 826 by South African scientists Gelderblom et al. in 1988, also known as fumonisins. Horsemycin, fumonisin, etc. The toxin is mainly produced by Fusarium moniliforme and F. proliferatum Matsushinma, and is a water-soluble secondary metabolite mainly polluting corn. In 1989, Laurent et al. isolated two toxic substances with similar structures from fumonisins, which were named fumonisins B respectively. 1 (FB 1 ) and fumonisin B 2 (FB 2 ). FB has been fo...

AI Technical Summary

Problems solved by technology

However, the pretreatment process of this method is cumbersome and the operation is complicated. Fumonisin needs to be derivatized before the column during the experiment, and usually requires professional personnel to operate, the equipment is expensive, and the detection process is time-consuming and expensive. It is not suitable for On-site rapid detection of a large number of samples; enzyme-linked immunoassay (ELISA) is more and more popular due to its strong specificity, high sensitivity, good accuracy, easy operation, and suitable for the detection of large batches of samples.

Although many researchers in scientific research institutions and colleges and universities have conducted research on the detection method of fumonisin B1 enzyme-linked immunoassay, the sensitivity of the detection and the stability of the kit are still far from practical application.

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