Kit and method for quantitative determination of Fumonisin B1 content in food by ELISA detection technique

An enzyme-linked immunoassay detection and fumonisin technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome pretreatment process, the stability distance of sensitivity kits, and complicated operation.

Inactive Publication Date: 2008-07-30
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, the pretreatment process of this method is cumbersome and the operation is complicated. Fumonisin needs to be derivatized before the column during the experiment, and usually requires professional personnel to operate, the equipment is expensive, and the detection process is time-consuming and expensive. It is not suitable for On-site rapid detection of a large number of samples; enzyme-linked immunoassay (ELISA) is more and

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  • Kit and method for quantitative determination of Fumonisin B1 content in food by ELISA detection technique

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Embodiment Construction

[0044] Illustrate the present invention below in conjunction with embodiment, the scheme of embodiment described here does not limit the present invention; Those skilled in the art can improve and change it according to the scheme of the present invention, and these improvements and changes all should be regarded as in the scope of the present invention Rather, the scope and spirit of the invention are defined by the claims.

[0045] 1. Preparation kit

[0046] 1. Fumonisins B 1 - Preparation of KLH antigen

[0047] Weigh 10 mg of macromolecular protein keyhole limpet hemocyanin (KLH) (hereinafter referred to as KLH), and use 10 mL of 0.2% glutaraldehyde (50% glutaraldehyde dissolved in 0.01mol / L PBS (buffer solution, hereinafter referred to as PBS) , pH 7.4) dissolved, stirred continuously at 4°C for 16 h, and dialyzed in 0.01 mol / L PBS (pH 7.4) for 48 h to remove excess glutaraldehyde. Take 1mg FB 1 Dissolve in 0.4mL of methanol, add dropwise to the activated KLH, stir a...

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Abstract

The invention relates to a reagent box for quantificationally detecting the content of fumonisin B1 in food by adopting an enzymoimmunoassay technology and the detecting method thereof. The reagent box includes the steps that a fumonisin B1-KLH antigen is prepared; a polyclonal fumonisin B1 antibody is prepared; an enzyme labeling antigen is prepared; a coated plate is prepared, and a reagent is prepared. The invention mainly adopts the enzymoimmunoassay technology to detect the fumonisin B1, can realize the aim of quantificationally detecting the content of fumonisin B1 in food and alimentary crops; and the reagent box has simple structure, convenient usage, cheap price and high sensitivity; the detecting method has strong specificity, high sensitivity, good veracity, and the sensitivity can reach gamma/kg grade.

Description

technical field [0001] The invention belongs to the technical field of food immune analysis, and relates to the detection of the content of fumonisin B1 in food and grain crops, in particular to a kit for quantitatively detecting the content of fumonisin B1 in food by enzyme-linked immunoassay technology and its detection method. Background technique [0002] Fumonisins (Fumonisins, FBs) are a group of structural analogs that were first isolated from the culture of F. verticilliodes (synonym: F. moniliforme Sheldon) MRC 826 by South African scientists Gelderblom et al. in 1988, also known as fumonisins. Horsemycin, fumonisin, etc. The toxin is mainly produced by Fusarium moniliforme and F. proliferatum Matsushinma, and is a water-soluble secondary metabolite mainly polluting corn. In 1989, Laurent et al. isolated two toxic substances with similar structures from fumonisins, which were named fumonisins B respectively. 1 (FB 1 ) and fumonisin B 2 (FB 2 ). FB has been fo...

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Application Information

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IPC IPC(8): G01N33/543G01N33/531
Inventor 王硕王向红郑文杰张燕权英
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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