Application of oroxylin A as biological pesticide in preventing and controlling corps diseases

A technology of kelp paper and biological pesticides, applied in the biological field, to achieve the effects of wide source of raw materials, good control effect, and obvious control effect

Inactive Publication Date: 2008-09-17
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The function of kelp A in plants has not

Method used

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  • Application of oroxylin A as biological pesticide in preventing and controlling corps diseases
  • Application of oroxylin A as biological pesticide in preventing and controlling corps diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Research on the inhibitory effect of compounds of the present invention on rice blast fungus

[0015] 1. Strains: Pyricularia grisea wo2-1 (compatibility with Lijiang Xintuan Heigu), sexual generation Magnaporthegrisea (Hebert) Barrnov

[0016] 2. Rice variety: Lijiang Xintuan Heigu rice variety, does not contain any blast resistance gene

[0017] The inhibitory activity of melaleucain A against Magnaporthe grisea was determined by the drug-containing medium method. M. oryzae was first activated on PDA (potato dextrose agar medium) for 72 hours before use. After Melaleutin A was dissolved with ethanol, it was diluted to different concentration gradients of 4000ug / g-40ug / g (that is, 10 times the concentration of each treatment), and the PDA was dissolved and cooled to 50°C. : 9 after mixing evenly (v / v), the drug-containing medium of 400ug / ml~4ug / ml is ready, and every treatment is poured into 4 flat plates (diameter 9cm), and after cooling, insert 6mm ba...

Embodiment 2

[0021] Embodiment 2: 50% aqueous solution.

[0022] After dissolving 50 parts (W / W) of melaleucain A with 10 parts of ethanol by weight, add 5 parts of 656H compounding aid for mixing, and add 35 parts of water to obtain a 50% water preparation.

Embodiment 3

[0023] Example 3: Indoor bioassay and field control test on vegetable cinerea (Botrytis cinerea).

[0024] According to the same method of Example 1, vegetable gray mold (Botrytis cinerea) is cultivated and bioassayed. In the field, it is applied after the onset of 3 true leaf stages of cucumbers, once every 7 days, 3 times in total. Survey after the disease is finalized. The results proved that celestialin A also had no direct antibacterial effect on rice blast on the culture medium. It also has obvious inhibitory effect on botrytis cinerea by using the detached leaf method. The results of field control experiments proved that it can effectively inhibit the occurrence of gray mold of cucumber in field.

[0025]

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Abstract

The invention relates to the application of biological pesticide using Oroxylin A as the active ingredient in crop disease control; the Oroxylin A is 5, 7-dihydroxy-6-methoxyflavone, which is a yellow needle like crystal with the melting point ranging from 231 DEG C to 232 DEG C used for the control of crop diseases caused by fungi. The Oroxylin A is the secondary metabolite of plants widely existing in the oroxylum indicum of bignoniaceae, the scutellaria of labiatae and other species. The application of biological pesticide using Oroxylin A as the active ingredient in crop disease control has the advantages that, 1 the reagent using Oroxylin A as the active ingredient is an anti fungal infection reagent characterized in low toxicity, low residue, compatibility with environment and other characteristics, belonging to new biological pesticide and having obvious control effect to rice blast and vegetable and fruit gray mold; 2 the reagent has no or low direct bactericidal effect or bacteriostatic action, but the objective of disease control can be achieved by inducing the disease resistance responses of plants; 3 raw material source is relatively wide, and the promotion and the application of the invention are facilitated.

Description

technical field [0001] The invention relates to the application of biopesticide with Oroxylin A (oroxylin A, oroxylin) as an active ingredient in preventing and treating crop diseases, and belongs to the field of biotechnology. Background technique [0002] With the global attention to resources and the environment, "organic agriculture" and "green food" have become the direction of agricultural development, and the search for and development of bio-rational pesticides with high efficiency, low toxicity, low residue and harmonious environment has gradually become the focus of pesticide research. new direction. Both rice and vegetables are crops grown on a large scale. Rice blast, also known as rice fever, is an important disease worldwide, and occurs in both the north and south rice regions of my country. In the area where the disease is endemic, the yield is generally reduced by 10%-20%, and the heavy one can reach 40%-50%. In the particularly serious field, there is even...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00
Inventor 喻大昭杨立军倪汉文王少南曾凡松向立波闫燕林张汉亦
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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