Application of oroxylin A as biological pesticide in preventing and controlling corps diseases
A technology of kelp paper and biological pesticides, applied in the biological field, to achieve the effects of wide source of raw materials, good control effect, and obvious control effect
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Embodiment 1
[0014] Embodiment 1: Research on the inhibitory effect of compounds of the present invention on rice blast fungus
[0015] 1. Strains: Pyricularia grisea wo2-1 (compatibility with Lijiang Xintuan Heigu), sexual generation Magnaporthegrisea (Hebert) Barrnov
[0016] 2. Rice variety: Lijiang Xintuan Heigu rice variety, does not contain any blast resistance gene
[0017] The inhibitory activity of melaleucain A against Magnaporthe grisea was determined by the drug-containing medium method. M. oryzae was first activated on PDA (potato dextrose agar medium) for 72 hours before use. After Melaleutin A was dissolved with ethanol, it was diluted to different concentration gradients of 4000ug / g-40ug / g (that is, 10 times the concentration of each treatment), and the PDA was dissolved and cooled to 50°C. : 9 after mixing evenly (v / v), the drug-containing medium of 400ug / ml~4ug / ml is ready, and every treatment is poured into 4 flat plates (diameter 9cm), and after cooling, insert 6mm ba...
Embodiment 2
[0021] Embodiment 2: 50% aqueous solution.
[0022] After dissolving 50 parts (W / W) of melaleucain A with 10 parts of ethanol by weight, add 5 parts of 656H compounding aid for mixing, and add 35 parts of water to obtain a 50% water preparation.
Embodiment 3
[0023] Example 3: Indoor bioassay and field control test on vegetable cinerea (Botrytis cinerea).
[0024] According to the same method of Example 1, vegetable gray mold (Botrytis cinerea) is cultivated and bioassayed. In the field, it is applied after the onset of 3 true leaf stages of cucumbers, once every 7 days, 3 times in total. Survey after the disease is finalized. The results proved that celestialin A also had no direct antibacterial effect on rice blast on the culture medium. It also has obvious inhibitory effect on botrytis cinerea by using the detached leaf method. The results of field control experiments proved that it can effectively inhibit the occurrence of gray mold of cucumber in field.
[0025]
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