Production of multivalent virus like particles
A virus-like, virus-based technology, applied in the direction of viruses, viral peptides, antiviral agents, etc., can solve the problems of VLPs lack of flexibility, reducing the number of antigenic inserts, and unable to control
Inactive Publication Date: 2008-10-15
DOW GLOBAL TECH LLC
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Problems solved by technology
Current methods for constructing multivalent VLPs are limited by: i) the inflexibility and inability to control the generation of VLPs in vivo due to inherent limitations in the insertability of capsid proteins, which reduces the number of possible combinations of antigenic inserts, or ii) ) A non-homogenous population of VLPs is generated due to the simple in vitro mixing of previously assembled VLP populations containing different inserts
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Abstract
The present invention is directed to the production and in vitro assembly of recombinant viral capsid proteins into virus like particles. In particular, the present invention provides rapid, scalable, and cost efficient methods for the production of multivalent virus like particles utilizing separate populations of capsid fusion peptides containing differing antigenic peptide inserts that are combined in vitro to produce homogenous populations of multivalent virus like particles. The virus like particles produced according to the present invention can be utilized to induce an immunological response in human or animal.
Description
Production of multivalent virus-like particles Cross-reference related applications This application claims the priority of the US provisional application US60 / 686,541 filed on June 1, 2005. Statement of government interest This application is bound by the contract with the cooperation agreement number 1-U01-AI054641-01, which was signed by the U.S. government, the National Institutes of Health and the National Institute of Allergy and Infectious Diseases (NIAID). Technical field The invention relates to the production and in vitro assembly of recombinant virus capsid proteins into virus-like particles. In particular, the present invention provides a rapid, scalable, and cost-effective method for producing multivalent virus-like particles, which uses an isolated population of capsid fusion peptides containing different antigenic peptide inserts, and these capsids are fused The peptides are combined in vitro to produce multivalent virus-like particles. The virus-like particles ...
Claims
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IPC IPC(8): A61K39/12C12N7/00
CPCA61K2039/5258A61K2039/70C07K14/32C12N2770/14023A61K2039/55561A61K39/295C12N7/00C07K14/005C12N2770/14022A61P31/12A61K38/16A61K39/00A61K39/12
Inventor L·拉索霍娃P·P·道J·费尔普斯
Owner DOW GLOBAL TECH LLC