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Process for abstracting momordica grosvenori polysaccharide

An extraction method and Luo Han Guo technology can be used in drug combination, allergic diseases and other directions, which can solve the problem of not being able to obtain Luo Han Guo polysaccharide, etc., and achieve the effects of saving raw materials, less investment and high yield

Inactive Publication Date: 2008-10-22
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese invention patent application 02128065.7 "Mogroside Extraction Process" mentions the use of membrane separation technology to obtain Luo Han Guo polysaccharides, but it is wrong to mention that Luo Han Guo polysaccharides are separated in the third membrane. The patent mentioned Luo Han Guo The polysaccharide is actually fructose, and Luo Han Guo polysaccharide cannot be obtained, because the molecular weight of Luo Han Guo polysaccharide is very large, generally more than tens of thousands, and the polysaccharide is trapped in the first layer of the membrane

Method used

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  • Process for abstracting momordica grosvenori polysaccharide
  • Process for abstracting momordica grosvenori polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 1 kg of mature fresh Luo Han Guo, and use the first method to extract the polysaccharides from Luo Han Guo:

[0030] (1) Momordica grosvenori is crushed;

[0031] (2) Add 5 kg of water, heat to boiling and soak for 1.5 hours, filter with a 250-mesh filter, continue to extract the slag, and extract 3 times in total, and combine the extracts;

[0032] (3) The extract was subjected to column chromatography with D101 or D102 or AB-8 macroporous resin to obtain a leakage liquid a and an eluent b, and the eluent was purified and dried to obtain 100 g of mogroside;

[0033] (4) The leakage liquid a is sequentially sent into the separation membrane under the working pressure of 0.15Mpa by the pressure pump, the molecular weight is 5000 Daltons, and the Luo Han Guo fiber, protein, pectin and polysaccharide in the Luo Han Guo are intercepted and separated to obtain the Luo Han Guo polysaccharide concentrate;

[0034] (5) Add ethanol to the polysaccharide concentrate of Luo ...

Embodiment 2

[0037] Take 1 kg of dried Luo Han Guo and use the second method to extract the polysaccharides from Luo Han Guo:

[0038] (1) Momordica grosvenori is crushed;

[0039] (2) Add 10 kg of water, heat to boiling and soak for 1.5 hours, filter with a 200-mesh filter, continue to extract the slag, and extract 3 times in total, and combine the extracts;

[0040] (3) The molecular weight that is sent to the separation membrane sequentially through the pressure pump under the working pressure of 0.1Mpa is 5000 Daltons, intercept and separate the Luo Han Guo cellulose, protein, pectin, polysaccharide in Luo Han Guo, and obtain the Luo Han Guo polysaccharide concentrate;

[0041] (4) Add ethanol to the concentrate until the alcohol content is 85%, let it sit and filter, centrifuge and precipitate, and obtain 6 grams of polysaccharide product, with a yield of 0.6%;

[0042] (5) The solution after membrane separation is separated by column chromatography with D101 or D102 or AB-8 macropor...

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Abstract

The invention discloses a method for extracting fructus momordicae amylose. Firstly, the fructus momordicae is crushed and is leached in boiled water for three times, each leaching lasts 1.5 hours, the leaching fluid is merged and is chromatographic through a macroporous resin column to obtain the fructus momordicae flavone glycoside, the film separation is performed in the transudate to obtain the fructus momordicae amylose product; or the film separation is performed in the extracted fluid to obtain the fructus momordicae amylose product, the separating medium is chromatographic through the macroporous resin column to obtain the fructus momordicae flavone glycoside. The two extracting methods can finish the steps of separation, extraction and concentration in one step and can realize the continuous production; the high-efficiency film separation technique is adopted, the yield rate of fructus momordicae amylose is high; the used solvent only adopts water and ethanol, thereby not generating any waste water or waste residue polluting the environment; moreover, the recycling is realized, thereby saving raw materials and greatly lowering the cost; the raw materials can be the waste fluid containing amyloase wasted by an enterprise, thereby improving the utilization rate of the effective component of fructus momordicae and the benefits of the enterprise; and the device is simple and the investment is small.

Description

Technical field: [0001] The invention relates to a method for extracting effective components of Luo Han Guo, in particular to a method for extracting Luo Han Guo polysaccharide. Background technique [0002] Luo Han Guo has been used in folk medicine for more than 300 years. It is cool in nature, sweet in taste, non-toxic, and has the effects of nourishing the lungs, relieving cough, cooling blood, moistening the intestines and laxative. Non-specific immunity has obvious immune enhancement effect. At present, there are also some patents reporting the extraction method of Luo Han Guo polysaccharides, such as Chinese invention patent application number 02113548.7 "Method for extracting and separating multiple components from Luo Han Guo". In this patent method, high-efficiency membrane separation technology is not used, and the products obtained by them are Mainly mogroside. Chinese invention patent application 02128065.7 "Mogroside Extraction Process" mentions the use of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 李俊廖日权苏小建黄艳何星存黄翠萍陆学文何星基
Owner GUANGXI NORMAL UNIV
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