Unlock instant, AI-driven research and patent intelligence for your innovation.

Mass spectrogram antibody kit of human chorionic gonadotrophin isomer and preparation method thereof

A technology of chorionic gonadotropin and isomers, which is applied in the field of protein detection, can solve the problem of inability to detect hCG isomers, fragments and terminal degradation products at the same time, standardized mass spectrometry antibody kits, and the lack of early Monitoring methods and other issues to achieve the effect of reducing experimental errors

Active Publication Date: 2012-10-10
许洋
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional hCG detection kits cannot simultaneously detect differential isomers such as hCGβcf, hCG-α and hCG-β
[0008] The above-mentioned hCG detection kits are of great value in evaluating the prognosis of ectopic pregnancy, but it is also found in clinical work that a considerable proportion of ectopic pregnancy patients cannot detect hCG isomers, fragments and terminal degradation products, which is of great importance for ectopic pregnancy. The prognosis assessment of pregnant patients lacks effective early monitoring methods
[0009] At present, there is no standardized mass spectrometry antibody kit for the detection of clinical hCG isomers, fragments and terminal degradation products when performing protein mass spectrometry analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mass spectrogram antibody kit of human chorionic gonadotrophin isomer and preparation method thereof
  • Mass spectrogram antibody kit of human chorionic gonadotrophin isomer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 of the mass spectrometry antibody kit for isomers of human chorionic gonadotropin proposed by the present invention includes:

[0090] A small tube of mass spectrometry standardized quality control serum (slurry) containing U9 buffer solution, 10 μl;

[0091] A small tube contains 30 μl of anti-hCG, anti-hCGβcf, anti-hCG-α and anti-hCG-β magnetic beads with an equal amount of 1 μg;

[0092] A small tube of buffer solution 300μl, the buffer solution is 50mM PBS, the pH value is 7.0;

[0093] A small tube of 10 μl of eluent, which is an aqueous solution of 1% trifluoroacetic acid;

[0094] A small tube of 5 μl of a saturated solution of energy absorbing molecules, which is composed of energy absorbing molecules dissolved in an aqueous solution containing 30% acetonitrile and 1% trifluoroacetic acid, and the energy absorbing molecules are cinnamic acid derivatives;

[0095] The above-mentioned small tubes were placed in a refrigerator at 4°C.

[0096] The prep...

Embodiment 2

[0103] Example 2 of the mass spectrometry antibody kit for isomers of human chorionic gonadotropin proposed by the present invention includes:

[0104] A small tube of mass spectrometry standardized quality control serum (slurry) containing U9 buffer solution, 30 μl;

[0105] A small tube containing 50 μl of anti-hCG, anti-hCGβcf, anti-hCG-α, and anti-hCG-β magnetic beads equal to 1 μg;

[0106] A small tube of buffer solution 500μl, the buffer solution is 100mM PBS, pH value is 7.4;

[0107] 50 μl of a small tube of eluent, which is an aqueous solution of 5% trifluoroacetic acid;

[0108] A small tube of energy absorbing molecular saturated solution 10 μl, the solution is composed of energy absorbing molecules dissolved in an aqueous solution containing 60% acetonitrile and 0.5% trifluoroacetic acid, the energy absorbing molecules use either sinapinic acid or dihydroxybenzoic acid ; The above small tubes were placed in a refrigerator at 4°C.

[0109] The preparation method...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for the mass spectrometry antibody of human chorionic gonadotropin isomers and a preparation method thereof, which pertain to the technical field of protein detection. The kit comprises a tubelet of mass spectrometry standard quality control serum containing a U9 buffer solution, a tubelet of magnetic beads containing equivalent 1Mug of anti hCG, anti hCGBetacf, anti hCG-Alpha and anti hCG-Beta, a tubelet of buffer solution, a tubelet of eluent and a tubelet of energy absorption molecular saturated solution and all the tubelets are put into a cold storage box atthe temperature of 4 DGE C. The invention can be applied to mass spectrometry antibody kits of in vitro sample detection and prognosis judgement. The method is accurate, convenient and fast.

Description

technical field [0001] The invention belongs to the technical field of protein detection, in particular to a method for preparing a kit for analyzing human chorionic gonadotropin isomers in biological samples of ectopic pregnancy based on mass spectrometry. Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, the identification of differences in proteins expressed in the human body can be used for diagnosis and screening of disease samples in vitro, and ultimately for drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations in the above aspects. It is difficult to identify and analyze chemical structures and p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/76G01N33/577G01N27/64
Inventor 许洋
Owner 许洋