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Multi-color optic-encoding siliceous skin nano-rods and method for preparing same

An optical encoding and nanorod technology, applied in the field of photoluminescent materials and their preparation, can solve the problems of harsh preparation conditions, poor hydrophilicity, dye leakage and other problems of quantum dots, and achieve complex process improvement and good biocompatibility Sexuality and hydrophilicity, the effect of less dye leakage

Inactive Publication Date: 2008-12-31
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymer materials are easy to swell and cause dye leakage, poor hydrophilicity, and easy to agglomerate in aqueous solution; quantum dots have problems such as harsh preparation conditions and high toxicity

Method used

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  • Multi-color optic-encoding siliceous skin nano-rods and method for preparing same
  • Multi-color optic-encoding siliceous skin nano-rods and method for preparing same
  • Multi-color optic-encoding siliceous skin nano-rods and method for preparing same

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Embodiment

[0028] A polychromatic optically coded silicon-shell nanorod is prepared by the following method:

[0029] (1) Polylysine with a molecular weight of 7 to 150,000 is dissolved in a pH 8.3, 0.1mol / L sodium bicarbonate solution to form solution A of 40 mg / mL; 5-carboxytetramethylrhodamine Succinimide ester (5-TAMRA, SE) was dissolved in anhydrous dimethyl sulfoxide (DMSO) to prepare 9.0×10 -3 mol / L solution B; add 30 μl solution B to 270 μl solution A, stir quickly evenly, keep stirring gently at room temperature for 2 hours, dialyze with 0.15mol / L NaCl solution to remove excess dye, then add pH 9.2, 0.1 Make up 1250 μl of mol / L carbonate buffer to obtain solution C;

[0030] (2) Dissolve fluorescein isothiocyanate (FITC) in anhydrous DMSO and prepare 7.6×10 -4 mol / L, 3.8×10 -4 mol / L, 1.9×10 -4 mol / L, 9.5×10 -5 mol / L, 4.8×10 -5 FITC / DMSO solutions with different concentrations of mol / L; take 45 μl solution A, add 162 μl, pH value 9.2, 0.1mol / L carbonate buffer and 23 μl 7.6...

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Abstract

The invention discloses a multi-color optical encoding nano-rod with silica shell. The multi-color optical encoding nano-rod takes the shape of a core shell, the shell is made of silicon dioxide and the kernel is fluorescence-encoded polylysine which contains fluorescent dye A and fluorescent dye B; the fluorescent dye A and fluorescent dye B are a fluorescence resonance energy transfer supplier-receptor pair. The invention also discloses a method for preparing the multi-color optical encoding nano-rod with silica shell, which includes the steps of enabling the fluorescent dye A and fluorescent dye B to react with the polylysine to prepare fluorescence-encoded polylysine, and wrapping the fluorescence-encoded polylysine, used as kernel material, in a silicon dioxide substrate through reverse microemulsion method; in this way, the multi-color optical encoding nano-rod with silica shell can be prepared. The multi-color optical encoding nano-rod with silica shell has the advantages of strong fluorescence intensity, good biological compatibility, good hydrophilicity, little leakage of dye and stable property.

Description

technical field [0001] The invention relates to a photoluminescence material in the field of nanotechnology and a preparation method thereof, in particular to a photoluminescence material which can be used for signal encoding in multiple biological analysis and fluorescence imaging and a preparation method thereof. Background technique [0002] The rapid development of life science technology has brought new challenges to analysis technology. Facing the increasing number of analytical objects, it is necessary to have corresponding analytical methods, especially multivariate analysis techniques that can simultaneously measure multiple components in the same sample. In multivariate analysis, it is often necessary to use a variety of fluorescent markers, and it is best to use only one wavelength of light to effectively excite the fluorescence of each marker, which requires that the selected markers have similar maximum excitation wavelengths and significant differences. Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/07G01N21/64G01N33/52
Inventor 王柯敏何晓晓秦迪岚谭蔚泓
Owner HUNAN UNIV
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