Mass long term storage process for marine higher fungi
A long-term preservation, large-scale technology, applied in the field of marine microbiology, can solve the problems of multiple devices, difficult to popularize and apply, and achieve the effect of easy operation
Inactive Publication Date: 2010-09-29
OCEAN UNIV OF CHINA
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
This method requires more equipment and cannot be used in general laboratories.
At the same time, it is difficult to popularize and apply due to the stricter conditions required during the resurrection and transfer of strains.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0011] More specific implementation steps of the present invention: (1) Prepare aged seawater: take natural seawater and store it in the dark for more than 3 months, and filter it through a 0.45 μm filter membrane during use to obtain aged seawater. Transfer the strains that need to be preserved to the old seawater agar culture plate (1000ml old seawater + 15 grams of agar powder), and cultivate at 15°C for one month to one and a half months (depending on the growth of the fungus). The aged seawater agar medium is a medium with oligotrophic conditions, conforms to the actual living environment of marine higher fungi, is used for cultivating fungi, can obtain stable and healthy fungal colonies, and is beneficial to prolong storage time. (2) Choose a cryopreservation tube with a capacity of 1.8ml, soak it in hydrochloric acid for disinfection, and rinse it with distilled water. Then, pipette 1.6ml of old sea water with a pipette gun and put it into a cryovial. (3) The cryoprese...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for preserving the marine higher fungus for a long time, which is characterized in that the strain needed to be preserved is adapted onto a stale seawater agar culture plate and then is cultivated under 15 DEG C for one month to one and a half months; then a chosen frozen pipe is soaked in hydrochloride to be disinfected and then is rinsed to be clean with distilled water; then 1.6 ml of the stale seawater is transferred into the frozen pipe by a pipette; the frozen pipe containing the stale seawater is sterilized by high pressure steam for twenty minutes, and then is taken out for spare use; then in the bacteria-free environment, a small piece of culture matrix with grown fungi, whose diameter is less than that of the frozen pipe, is cut by a sterilized scalpel, and then is placed inside the frozen pipe to be soaked in the stale seawater; finally, the cap of the frozen pipe is screwed on, the frozen pipe is filled inside a box of the frozen pipe, then the box of the frozen pipe is preserved in dark condition of 5 DEG C. With convenient and easy operation, the method solves the problem that the marine higher fungus in preservation degenerates and inactivates easily, which causes the experimental medicine and the space resource to be wasted.
Description
technical field [0001] The invention belongs to the field of marine microbiology, and relates to a method for long-term preservation of large quantities of marine higher fungi. Background technique [0002] The isolated fungus should be preserved in an appropriate way to maintain its vitality and original character, and at the same time avoid contamination by miscellaneous bacteria. Marine higher fungi are an important class of marine microbial resources. Due to the late start of research on this type of microorganisms, only more than 500 species of marine higher fungi have been reported so far. Compared with terrestrial fungi, there is no special research on the preservation technology of marine higher fungi. At present, there is no mature method for the preservation of marine higher fungal species, mainly referring to the preservation technology of terrestrial fungi. [0003] At present, there are many preservation methods for terrestrial fungi, mainly in the following t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李伟唐学玺孙开明
Owner OCEAN UNIV OF CHINA