Dendrobium nobi tissue culturing and planting method

Abstract

The present invention relates to a tissue culture production technology and a cultivation method of dendrobium nobile, which include the following steps: inserting seeds of dendrobium nobile into a culture medium of N6 + NAA 0.3mg / L + potato juice 20%(V / V) + agar-agar 5g / L to perform cultivation, at 22-25 DEG C, for 10-12 hours of average diurnal light, under 1800-2500lx intensity of illumination, for 15-30 days; transplanting the cultivated seeds to a culture medium of N6 + BA 0.2mg / L + potato juice 20%(V / V) + agar-agar 5g / L to perform cultivation for 25 days; transplanting the cultivated seeds to a culture medium of N6 + NAA 0.2mg / L + banana juice 10%(V / V) + potato juice 20%(V / V) + agar-agar 6g / L for two months to form big seedlings; planting the big seedlings in wood chips which mixed blue stones and decomposed organic fertilizers for hardening-seedling, which begins in every March for one year, and spraying water in time; and transplanting the hardened seedlings to open ground or greenhouse to perform cultivation with a transplanting medium of shale + moss and to fasten the seedlings by cable fasteners, under appropriate humiture and light irradiation, managing with GAP qualitative specification.

Description

1. Technical field [0001] The patent application of the present invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to a tissue culture production technology and cultivation method of medicinal Dendrobium nobile. 2. Background technology [0002] Dendrobium nobile is the first of the three major species of Dendrobium recorded in "Chinese Pharmacopoeia", and it has been recorded in "Shen Nong Herbal Classic" and "Compendium of Materia Medica". The stem of Dendrobium nobile is used as medicine, which has the functions of benefiting stomach and promoting body fluid, nourishing yin and clearing heat, relieving pain, clearing throat, improving eyesight, lowering blood fat, and blood pressure. The main raw material of products such as "Dendrobium Capsules". The "Mailuoning" series of products developed with Dendrobium as the main raw material has been designated by the state as an essential medicine for cardiovascular ...

AI Technical Summary

Problems solved by technology

It is currently the largest base of Dendrobium nobile in China, but its planting scale and output are far from meeting the huge demand of the market

[0004] However, in terms of planting methods, most drug farmers continue to use the traditional method, using seedlings for division and cutting cultivation, which consumes a large amount of Dendrobium seedlings, resulting in scarcity of germplasm resources, low reproduction coefficient, slow development speed, and the scale of planting area cannot be effectively expanded.

Moreover, due to the backward cultivation technology and extensive and simple management, the survival rate of Dendrobium dendrobium is low, the growth cycle is long, the yield is low, and the benefit is poor.