Construction method of animal model with diabetes skin ulcer

A technology for skin ulcers and animal models, which is used in pharmaceutical formulations, preparations for in vivo experiments, animal husbandry, etc. Simple, high molding rate, low cost effect

Inactive Publication Date: 2009-01-21
SHANGHAI NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Although the above-mentioned experimental animal models of diabetic skin ulcers play a guiding and reference role in the research on the mechanism of diabetic skin u

Method used

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Examples

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Comparison scheme
Effect test

Embodiment

[0021] 1. Experimental equipment

[0022] Blood glucose measuring instrument (Johnson & Johnson), 9cm diameter petri dish, precision electronic balance, 10μl micro-sampler; 1ml syringe, 0.5ml dropper; centrifugal sedimentation device; conventional ophthalmic surgical instruments; Nikon digital camera, Soxhlet extractor, etc. .

[0023] 2. Experimental animals and materials

[0024] 2.1 Experimental animals

[0025] Kunming mice, clean grade, male, weighing 18.32±1.63g, were provided by the Animal Experiment Center of Shanghai Institute of Biological Products.

[0026] 2.2 Experimental materials

[0027] Analyze pure absolute ethanol, Alloxan, 25% urethane, paraformaldehyde, picric acid, distilled water, sterile iodine, sterile ethanol, normal saline, sterile gauze and sterile cotton swabs.

[0028] 3. Experimental method

[0029] 24 above-mentioned Kunming mice were selected, randomly divided into 2 groups, 12 in each group, and marked respectively; intraperitoneal inject...

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PUM

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Abstract

The invention discloses a method for constructing an animal model of skin ulcer with diabetes. A Kunming white mouse is chosen and the abdominal cavity is injected with alloxan reagent, the mid lower portion of the back is unhaired, sterilized and cut deep to be under the skin, then the white mouse is fed in a single case and fixed amount of water and food is supplied. After fast for 12 hours, blood is taken from the tail of the white mouse and the blood sugar value is tested by a blood sugar tester to be properly more than or equal to 10.0mmol/L. The statistical analysis of body surface wound observation and the histological wound observation of the experimental animal model proves that the white mouse model of skin ulcer with diabetes that is constructed by alloxan is feasible and can be taken as the study model of pathogenesis and drug effect mechanism of skin ulcer with diabetes. Compared with the prior art, the white mouse model of skin ulcer with diabetes constructed by the invention has the advantages of simple model constructing method, high model forming ratio, low cost, short cycle and good reproducibility, etc.

Description

technical field [0001] The invention relates to a method for constructing a diabetic skin ulcer animal model, in particular to a method for constructing a mouse diabetic skin ulcer model. Background technique [0002] Diabetic skin ulcer is a relatively stubborn chronic ulcer caused by foot skin lesions caused by diabetes. If it is not treated in time, it will develop deep and even amputate. According to statistics, more than 50% of non-traumatic amputations are caused by diabetic skin ulcers, and 3% to 6% of diabetic patients develop foot ulcers every year. Multiple factors and uncertainties restrict its treatment. If we can really understand the mechanism of diabetic skin ulcers, then there will be a new leap forward in the treatment of diabetic skin ulcers. [0003] Animal models of human diseases are an extremely important experimental method and means in modern medical research. Using animal models of human diseases to study human diseases can carry out various experi...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027
Inventor 万平李利珍赵梅君汪寅
Owner SHANGHAI NORMAL UNIVERSITY
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